Nextflow stdout:

WARN: No DADA2 cutoffs were specified (`--trunclenf` & `--trunclenr`), therefore reads will be truncated where median quality drops below 25 (defined by `--trunc_qmin`) but at least a fraction of 0.75 (defined by `--trunc_rmin`) of the reads will be retained.
The chosen cutoffs do not account for required overlap for merging, therefore DADA2 might have poor merging efficiency or even fail.

WARN: A process with name 'ANCOMBC_FORMULA_ASV' is defined more than once in module script: /home/runner/work/ampliseq/ampliseq/./workflows/../subworkflows/local/qiime2_ancom.nf -- Make sure to not define the same function as process
WARN: A process with name 'ANCOMBC_FORMULA_TAX' is defined more than once in module script: /home/runner/work/ampliseq/ampliseq/./workflows/../subworkflows/local/qiime2_ancom.nf -- Make sure to not define the same function as process
WARN: Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value`
WARN: The following invalid input values have been detected:

* --baseDir: /home/runner/work/ampliseq/ampliseq
* --base-dir: /home/runner/work/ampliseq/ampliseq
* --outputDir: /home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/output
* --output-dir: /home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/output


WARN: The operator `first` is useless when applied to a value channel which returns a single value by definition
ERROR ~ Error executing process > 'NFCORE_AMPLISEQ:AMPLISEQ:METADATA_PAIRWISE (Metadata.tsv)'

Caused by:
  Process `NFCORE_AMPLISEQ:AMPLISEQ:METADATA_PAIRWISE (Metadata.tsv)` terminated with an error exit status (125)

Command executed:

  metadata_pairwise.r Metadata.tsv
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_AMPLISEQ:AMPLISEQ:METADATA_PAIRWISE":
      R: $(R --version 2>&1 | sed -n 1p | sed 's/R version //' | sed 's/ (.*//')
  END_VERSIONS

Command exit status:
  125

Command output:
  (empty)

Command error:
  Unable to find image 'quay.io/biocontainers/bioconductor-dada2:1.30.0--r43hf17093f_0' locally
  docker: Error response from daemon: received unexpected HTTP status: 502 Bad Gateway.
  See 'docker run --help'.

Work dir:
  /home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/work/1d/6d08aa1d19bceee2e9fd6a7d52a21e

Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`

 -- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/meta/nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

 -- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/meta/nextflow.log' file for details
ERROR ~ Cannot invoke method getAt() on null object

 -- Check script './workflows/../subworkflows/local/dada2_preprocessing.nf' at line: 61 or see '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/meta/nextflow.log' file for more details
WARN: Killing running tasks (2)
ERROR ~ Unexpected error [NullPointerException]

 -- Check script './subworkflows/local/utils_nfcore_ampliseq_pipeline/../../nf-core/utils_nfcore_pipeline/main.nf' at line: 106 or see '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/meta/nextflow.log' file for more details
WARN: Got an interrupted exception while taking agent result | java.lang.InterruptedException
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

 -- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/meta/nextflow.log' file for details
Nextflow stderr: