Ampliseq Version 2.11.0 #1487
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Aug 5, 2024 in 0s.
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Assertion failed:
11 of 22 assertions failed
Raw output
Nextflow stdout:
ERROR ~ Error executing process > 'NFCORE_AMPLISEQ:AMPLISEQ:DADA2_PREPROCESSING:DADA2_FILTNTRIM (sampleID_1a)'
Caused by:
Process `NFCORE_AMPLISEQ:AMPLISEQ:DADA2_PREPROCESSING:DADA2_FILTNTRIM (sampleID_1a)` terminated with an error exit status (125)
Command executed:
#!/usr/bin/env Rscript
suppressPackageStartupMessages(library(dada2))
out <- filterAndTrim("sampleID_1a.trimmed.trim.fastq.gz", "sampleID_1a.filt.fastq.gz",
truncLen = 0,
maxN = 0, truncQ = 2, trimRight = 0, minQ = 0, rm.lowcomplex = 0, orient.fwd = NULL, matchIDs = FALSE, id.sep = "\\s", id.field = NULL, n = 1e+05, OMP = TRUE, qualityType = "Auto",maxEE = 2,trimLeft = 0, minLen = 50, maxLen = Inf, rm.phix = TRUE,
compress = TRUE,
multithread = 2,
verbose = TRUE)
out <- cbind(out, ID = row.names(out))
# If no reads passed the filter, write an empty GZ file
if(out[2] == '0'){
for(fp in c("sampleID_1a.filt.fastq.gz")){
print(paste("Writing out an empty file:", fp))
handle <- gzfile(fp, "w")
write("", handle)
close(handle)
}
}
write.table( out, file = "sampleID_1a.filter_stats.tsv", sep = "\t", row.names = FALSE, quote = FALSE, na = '')
write.table(paste('filterAndTrim truncLen = 0','maxN = 0, truncQ = 2, trimRight = 0, minQ = 0, rm.lowcomplex = 0, orient.fwd = NULL, matchIDs = FALSE, id.sep = "\\s", id.field = NULL, n = 1e+05, OMP = TRUE, qualityType = "Auto",maxEE = 2,trimLeft = 0, minLen = 50, maxLen = Inf, rm.phix = TRUE',sep=","), file = "filterAndTrim.args.txt", row.names = FALSE, col.names = FALSE, quote = FALSE, na = '')
writeLines(c("\"NFCORE_AMPLISEQ:AMPLISEQ:DADA2_PREPROCESSING:DADA2_FILTNTRIM\":", paste0(" R: ", paste0(R.Version()[c("major","minor")], collapse = ".")),paste0(" dada2: ", packageVersion("dada2")) ), "versions.yml")
Command exit status:
125
Command output:
(empty)
Command error:
Unable to find image 'quay.io/biocontainers/bioconductor-dada2:1.30.0--r43hf17093f_0' locally
docker: Error response from daemon: Head "https://quay.io/v2/biocontainers/bioconductor-dada2/manifests/1.30.0--r43hf17093f_0": received unexpected HTTP status: 502 Bad Gateway.
See 'docker run --help'.
Work dir:
/home/runner/work/ampliseq/ampliseq/.nf-test/tests/c109527dc54db1247070e58b1c6bb28b/work/be/a9e0a3a6ab7cc3bfd2e681f7ada22e
Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line
-- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/c109527dc54db1247070e58b1c6bb28b/meta/nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting
-- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/c109527dc54db1247070e58b1c6bb28b/meta/nextflow.log' file for details
Nextflow stderr:
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