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Ampliseq Version 2.11.0 #1487

Ampliseq Version 2.11.0

Ampliseq Version 2.11.0 #1487

GitHub Actions / JUnit Test Report failed Aug 5, 2024 in 0s. View latest attempt.

1 tests run, 0 passed, 0 skipped, 1 failed.

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Assertion failed: 

11 of 22 assertions failed
Raw output
Nextflow stdout:

ERROR ~ Error executing process > 'NFCORE_AMPLISEQ:AMPLISEQ:DADA2_PREPROCESSING:DADA2_FILTNTRIM (sampleID_1a)'

Caused by:
  Process `NFCORE_AMPLISEQ:AMPLISEQ:DADA2_PREPROCESSING:DADA2_FILTNTRIM (sampleID_1a)` terminated with an error exit status (125)


Command executed:

  #!/usr/bin/env Rscript
  suppressPackageStartupMessages(library(dada2))
  
  out <- filterAndTrim("sampleID_1a.trimmed.trim.fastq.gz", "sampleID_1a.filt.fastq.gz",
      truncLen = 0,
      maxN = 0, truncQ = 2, trimRight = 0, minQ = 0, rm.lowcomplex = 0, orient.fwd = NULL, matchIDs = FALSE, id.sep = "\\s", id.field = NULL, n = 1e+05, OMP = TRUE, qualityType = "Auto",maxEE = 2,trimLeft = 0, minLen = 50, maxLen = Inf, rm.phix = TRUE,
      compress = TRUE,
      multithread = 2,
      verbose = TRUE)
  out <- cbind(out, ID = row.names(out))
  
  # If no reads passed the filter, write an empty GZ file
  if(out[2] == '0'){
      for(fp in c("sampleID_1a.filt.fastq.gz")){
          print(paste("Writing out an empty file:", fp))
          handle <- gzfile(fp, "w")
          write("", handle)
          close(handle)
      }
  }
  
  write.table( out, file = "sampleID_1a.filter_stats.tsv", sep = "\t", row.names = FALSE, quote = FALSE, na = '')
  write.table(paste('filterAndTrim	truncLen = 0','maxN = 0, truncQ = 2, trimRight = 0, minQ = 0, rm.lowcomplex = 0, orient.fwd = NULL, matchIDs = FALSE, id.sep = "\\s", id.field = NULL, n = 1e+05, OMP = TRUE, qualityType = "Auto",maxEE = 2,trimLeft = 0, minLen = 50, maxLen = Inf, rm.phix = TRUE',sep=","), file = "filterAndTrim.args.txt", row.names = FALSE, col.names = FALSE, quote = FALSE, na = '')
  writeLines(c("\"NFCORE_AMPLISEQ:AMPLISEQ:DADA2_PREPROCESSING:DADA2_FILTNTRIM\":", paste0("    R: ", paste0(R.Version()[c("major","minor")], collapse = ".")),paste0("    dada2: ", packageVersion("dada2")) ), "versions.yml")

Command exit status:
  125

Command output:
  (empty)

Command error:
  Unable to find image 'quay.io/biocontainers/bioconductor-dada2:1.30.0--r43hf17093f_0' locally
  docker: Error response from daemon: Head "https://quay.io/v2/biocontainers/bioconductor-dada2/manifests/1.30.0--r43hf17093f_0": received unexpected HTTP status: 502 Bad Gateway.
  See 'docker run --help'.

Work dir:
  /home/runner/work/ampliseq/ampliseq/.nf-test/tests/c109527dc54db1247070e58b1c6bb28b/work/be/a9e0a3a6ab7cc3bfd2e681f7ada22e

Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line

 -- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/c109527dc54db1247070e58b1c6bb28b/meta/nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

 -- Check '/home/runner/work/ampliseq/ampliseq/.nf-test/tests/c109527dc54db1247070e58b1c6bb28b/meta/nextflow.log' file for details
Nextflow stderr: