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Use nested chunk store for labels #82

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merged 5 commits into from
Oct 12, 2021

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will-moore
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@will-moore will-moore commented Sep 16, 2021

This uses nested chunks when exporting labels from OMERO.
Without this, the Image data is nested but labels are not - so they are ignored in napari.

Also, it updates labels to v0.3 (corresponding to #70 for image data) where we
discard any 'empty' dimensions. This means that the image data and labels data have same number of dimensions so that they display correctly in napari.

Testing setup

You'll need a python environment with: omero-cli-zarr, ome-zarr-py, napari-ome-zarr installed.
If we just want the released versions of everything:

Create an environment, using this environment.yml:

channels:
  - ome
  - conda-forge
  - defaults
dependencies:
  - python
  - wheel
  - omero-py == 5.10.0
  - zarr == 2.9.5
  - napari[all]
  - pip
  - pip:
      - omero-cli-zarr
      - napari-ome-zarr
conda env create -f environment.yml -n ome_ngff
conda activate ome_ngff

To get a specific branch of https://github.com/ome/omero-cli-zarr or https://github.com/ome/ome-zarr-py or https://github.com/ome/napari-ome-zarr:

  • clone the repo and checkout the branch you need
  • cd into the repo
  • pip uninstall omero-cli-zarr # or ome-zarr or napari-ome-zarr
  • pip install -e . # reinstall from the current branch

To test with https://idr.openmicroscopy.org/webclient/?show=image-6001247:

With ome-zarr-py from ome/ome-zarr-py#114 needed for down-sampling of labels with fewer than 5D.

$ omero zarr export Image:6001247
$ omero zarr masks Image:6001247

The shape of the labels should match the image data (same number of dimensions) although size_c for channels will be 1.

This also needs ome/napari-ome-zarr#16 for viewing:

$ napari 6001247.zarr

"""
Create an FSStore instance that supports nested storage of chunks.
"""
return FSStore(
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I assume this logic could be delegated to ome_zarr.format down the line? This would also make the format version used by this plugin more explicit.

Immediate caveat looking at https://github.com/ome/ome-zarr-py/blob/62b49da4b7200384dac8dec8d9fce48bd727a967/ome_zarr/format.py#L101-L125 is that normalize_keys would need to configurable (unless it could be set to True here?).

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It was set to False because otherwise path with A/1 was normalised to a/1: #59 (comment)

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That may have been because upstream was doing something wonky. Again, I think that's all settled:

zarr-developers/zarr-python#755

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If I want this repo to depend on that fix zarr>=2.8.3 I'd either need to update that in ome-zarr-py (currently "zarr>=2.8.1") or add zarr as a dependency to this repo?

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Happy for it to happen in either as long as we eventually unify. fwiw, zarr is now at 2.10.*

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sbesson commented Sep 16, 2021

So that I understand the compatibility matrix here, is napari effectively only support version 0.2+ of the multiscales specification?

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No, latest napari + napari_ome_zarr still supports e.g.

https://s3.embassy.ebi.ac.uk/idr/zarr/v0.1/6001240.zarr

But before this PR, the labels didn't use nested chunks but the image data did. It looks like napari expects both to be read the same way, so the labels are all black (unless they are also nested).

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NB: Newer versions of zarr should ignore the requested chunk and use the .zarray metadata correctly.

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sbesson commented Sep 16, 2021

But before this PR, the labels didn't use nested chunks but the image data did. It looks like napari expects both to be read the same way, so the labels are all black (unless they are also nested).

Cheers, that makes sense although to the best of my knowledge there is no current enforcement that the label image should have the same multiscales version as the image?

@sbesson sbesson closed this Sep 17, 2021
@sbesson sbesson reopened this Sep 17, 2021
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sbesson commented Sep 17, 2021

Overall no objection from my side to unifying the writing behavior of this tool so that both images and label images use the same version of the spec. Happy to see this released as 0.1.1.

@sbesson sbesson requested a review from joshmoore September 17, 2021 08:59
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One major question before we merge this.

src/omero_zarr/masks.py Outdated Show resolved Hide resolved
@@ -18,3 +20,16 @@ def print_status(t0: int, t: int, count: int, total: int) -> None:
eta = "NA"
status = f"{percent_done:.2f}% done, ETA: {eta}"
print(status, end="\r", flush=True)


def open_store(name: str) -> FSStore:
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Generally ok with this refactoring, but if it's going public we need to keep ome/ome-zarr-py#110 and similar in mind. i.e. we may not want to control this in the future.

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sbesson commented Sep 24, 2021

In order to fix the napari bug, this PR migrates the open_store logic into a new public API. This has impact on other PRs like #83. To minimize conflicts and reduce future pain, I would propose we make a decision on integrating these changes sooner rather than later. @will-moore can you comment on #82 (review) ?

EDIT: @will-moore pointed out that the commit associated to #82 (comment) got reverted in #82 (comment). This should be ready for a final round of review

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This pull request has been mentioned on Image.sc Forum. There might be relevant details there:

https://forum.image.sc/t/prepping-and-including-roi-masks/48750/24

@sbesson sbesson requested review from dominikl and pwalczysko October 1, 2021 14:23
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Addition of

- conda-forge to the channels block of the environment.yml seems to be necessary, otherwise see https://openmicroscopy.slack.com/archives/C0K5EED3R/p1633099632195100
cc @khaledk2 @dominikl

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pwalczysko commented Oct 1, 2021

Built the environment as prescribed in the header.
Included in it the present PR, the ome/napari-ome-zarr#16 and the ome/ome-zarr-py#114.
Exported the image from IDR as per the PR header, with the labels.
Opened the image in napari as per PR header.
All works, viewing in napari with no probs, labels are there, as well as the two channels of the image. The labels perfectly match the intensities in the image, as verified by switching on and off the displays.

This looks like the workflow 1 in https://docs.google.com/document/d/1LOihtEZWTA7DKsA6zXG1OlQuGTuV7SxAa5OVFSQNeDc/edit#bookmark=id.aygdx8up5vho is tested ?
Screenshot 2021-10-01 at 16 46 08

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dominikl commented Oct 8, 2021

Exported images look fine in napari. But I don't really understand the directory structure any more. Does that mean the image has 3 resolution (0,1,2) (for a 253 x 210 px image!?) and the labels only have one resolution (0)? What are the subdirectories 0,1,2,3 beneath labels/0 ?
Screenshot 2021-10-08 at 14 57 34

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will-moore commented Oct 8, 2021

@dominikl
I tend to use my editor (VSCode) to inspect the .zarr data since you can then see and read the hidden files.
E.g. you can see that the smallest '2' resolution has a .zarray of:

Screenshot 2021-10-08 at 16 01 53

The 0 is just the name of the first labels array, and it has 4 resolutions, the smallest being:

"shape": [
        1,
        257,
        26,
        31
    ],

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@pwalczysko With your test above (#82 (comment)) that is a successful test of ome/napari-ome-zarr#16, so could you give a 👍 on that PR too? Thanks.

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$ ssh -A ome-zarr-dev1.openmicroscopy.org
$ conda activate omero_zarr_export
$ pip freeze | grep zarr
ome-zarr==0.1.0
omero-cli-zarr @ git+https://github.com/will-moore/omero-cli-zarr.git@8dc47076e5bd22f5ffc1244d968f140873e972f0
zarr==2.9.5

# update ome-zarr-py and omero-cli-zarr to install from correct branches...
$ pip uninstall ome-zarr
$ pip install git+https://github.com/will-moore/ome-zarr-py.git@write_scale_less_than_5D
$ pip uninstall omero-cli-zarr
$ pip install git+https://github.com/will-moore/omero-cli-zarr.git@labels_chunks_nested

# export... - Use commit in the dir name, so we know what created it...
$ cd /uod/idr/objectstore/minio/idr/v0.3/
$ mkdir idr0047
$ cd idr0047
$ mkdir 3dc087c
$ cd 3dc087c

$ omero zarr export Image:4496763
Exporting to 4496763.zarr (0.3)
Finished.

$ omero zarr masks Image:4496763
Export Masks on Image: Exp1_rep1_0min_im1.tif
Found 430 mask shapes in 430 ROIs
Unique dimensions: {'T': {None}, 'C': {None}, 'Z': {None}}
source_image 4496763.zarr
Ignoring dimensions {'T', 'Z', 'C'}
Created 4496763.zarr/labels/0

Data is available at https://minio-dev.openmicroscopy.org/idr/v0.3/idr0047/3dc087c/4496763.zarr and can be opened
locally with ome/napari-ome-zarr#16:

$ napari  https://minio-dev.openmicroscopy.org/idr/v0.3/idr0047/3dc087c/4496763.zarr

Turning on the labels:

Screenshot 2021-10-11 at 16 22 26

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NB: In the previous example, the masks on Image https://idr.openmicroscopy.org/webclient/img_detail/4496763/ don't have any Z-index (or T or C) so in IDR they are shown across all Z sections.

These dimensions are ignored when exporting labels (see output above Ignoring dimensions {'T', 'Z', 'C'}) and we are left with a 4D array - NB: this probably could/should be 2D:

$ curl https://minio-dev.openmicroscopy.org/idr/v0.3/idr0047/3dc087c/4496763.zarr/labels/0/0/.zarray
{
    "chunks": [
        1,
        1,
        256,
        512
    ],
    "compressor": {
        "blocksize": 0,
        "clevel": 5,
        "cname": "lz4",
        "id": "blosc",
        "shuffle": 1
    },
    "dimension_separator": "/",
    "dtype": "<i8",
    "fill_value": 0,
    "filters": null,
    "order": "C",
    "shape": [
        1,
        1,
        2048,
        2048
    ],
    "zarr_format": 2

This explains why it is only visible for the first Z-index in napari above.

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I also exported https://minio-dev.openmicroscopy.org/idr/v0.3/idr0052/3dc087c/5514374.zarr from idr0052 but mask export fails since the masks overlap:

Exception: Mask 1369052 overlaps with existing labels
$ omero zarr masks Image:5514374
Using session for [email protected]:4064. Idle timeout: 10 min. Current group: Public
Export Masks on Image: cell0005_R0001.companion.ome
Found 2183 mask shapes in 80 ROIs
Unique dimensions: {'T': {0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39}, 'C': {None}, 'Z': {1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29}}
source_image 5514374.zarr
Ignoring dimensions {'C'}
Traceback (most recent call last):
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/bin/omero", line 11, in <module>
    sys.exit(main())
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero/main.py", line 125, in main
    rv = omero.cli.argv()
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero/cli.py", line 1784, in argv
    cli.invoke(args[1:])
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero/cli.py", line 1222, in invoke
    stop = self.onecmd(line, previous_args)
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero/cli.py", line 1299, in onecmd
    self.execute(line, previous_args)
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero/cli.py", line 1381, in execute
    args.func(args)
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero_zarr/cli.py", line 65, in _wrapper
    return func(self, *args, **kwargs)
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero_zarr/cli.py", line 236, in masks
    image_shapes_to_zarr(image, ["Mask"], args)
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero_zarr/masks.py", line 167, in image_shapes_to_zarr
    saver.save(list(masks.values()), args.label_name)
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero_zarr/masks.py", line 300, in save
    labels, fill_colors, properties = self.masks_to_labels(
  File "/lifesci/groups/jrs/wmoore/miniconda3/envs/omero_zarr_export/lib/python3.9/site-packages/omero_zarr/masks.py", line 513, in masks_to_labels
    raise Exception(
Exception: Mask 1369052 overlaps with existing labels

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$ omero zarr masks Image:11511036
Using session for [email protected]:4064. Idle timeout: 10 min. Current group: Public
Export Masks on Image: 1X [2]
Found 52 mask shapes in 52 ROIs
Unique dimensions: {'T': {None}, 'C': {None}, 'Z': {None}}
source_image 11511036.zarr
Ignoring dimensions {'C', 'T', 'Z'}
Created 11511036.zarr/labels/0
napari https://minio-dev.openmicroscopy.org/idr/v0.3/idr0095/3dc087c/11511036.zarr

Zoomed-in to see labels:

Screenshot 2021-10-11 at 17 12 40

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(ome_ngff) pwalczysko@ls31618~/Work/omero-cli-zarr$ omero zarr masks Image:5514374
Using session for [email protected]:4064. Idle timeout: 10 min. Current group: Public
Export Masks on Image: cell0005_R0001.companion.ome
Found 2183 mask shapes in 80 ROIs
Unique dimensions: {'T': {0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39}, 'C': {None}, 'Z': {1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29}}
source_image 5514374.zarr
Ignoring dimensions {'C'}
Traceback (most recent call last):
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/bin/omero", line 11, in <module>
    sys.exit(main())
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/main.py", line 125, in main
    rv = omero.cli.argv()
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1784, in argv
    cli.invoke(args[1:])
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1222, in invoke
    stop = self.onecmd(line, previous_args)
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1299, in onecmd
    self.execute(line, previous_args)
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1381, in execute
    args.func(args)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/cli.py", line 65, in _wrapper
    return func(self, *args, **kwargs)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/cli.py", line 236, in masks
    image_shapes_to_zarr(image, ["Mask"], args)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/masks.py", line 167, in image_shapes_to_zarr
    saver.save(list(masks.values()), args.label_name)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/masks.py", line 300, in save
    labels, fill_colors, properties = self.masks_to_labels(
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/masks.py", line 513, in masks_to_labels
    raise Exception(
Exception: Mask 1369052 overlaps with existing labels

@will-moore
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@pwalczysko Yep, that's what I saw at #82 (comment)
We could handle that better, but it's not really related to this PR.

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pwalczysko commented Oct 11, 2021

napari 4496763.zarr - specific issue as follows:
In napari, the labels seem to be associated with first z plane only. If the labels are put on display on any other plane, then they do not show. This is in contrast with napari /6001247.zarr where the labels show on every z plane. Checked the masks on the 4496763 image in IDR and they do not seem to be associated with any plane.

Screenshot 2021-10-11 at 17 27 42

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In summary,

@will-moore
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The strategy for omero zarr masks export when the masks in OMERO have no Z/T index is to only output a single plane, instead of outputting potentially hundreds of identical planes and increasing the size of the data many times.
So, instead of a labels data that matches the dimensions of the Image, we have size-Z and/or size-T to be 1.

It would be nice if there was some way of getting napari to display the same labels across all Z/T indices without actually duplicating the data. But regardless, this isn't a new issue related to this PR.

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@pwalczysko I remembered that there is a way of handling overlapping masks as described at https://github.com/ome/omero-cli-zarr#masks (using an idr0052 image as an example).
Using this approach, exported masks for this image:

napari https://minio-dev.openmicroscopy.org/idr/v0.3/idr0052/3dc087c/5514374.zarr

Screenshot 2021-10-12 at 09 21 20

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For me, the workflow described in https://github.com/ome/omero-cli-zarr#masks for overlapping masks ended with error

omero zarr masks Image:5514374 --label-map=5514374.rois
Using session for [email protected]:4064. Idle timeout: 10 min. Current group: Public
Export Masks on Image: cell0005_R0001.companion.ome
Found 2183 mask shapes in 80 ROIs
Error parsing 5514374.rois: 1369132
Label map: Cell (count: 0)
Unique dimensions: {'T': set(), 'C': set(), 'Z': set()}
source_image 5514374.zarr
Ignoring dimensions set()
Traceback (most recent call last):
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/bin/omero", line 11, in <module>
    sys.exit(main())
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/main.py", line 125, in main
    rv = omero.cli.argv()
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1784, in argv
    cli.invoke(args[1:])
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1222, in invoke
    stop = self.onecmd(line, previous_args)
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1299, in onecmd
    self.execute(line, previous_args)
  File "/Users/pwalczysko/miniconda3/envs/ome_ngff/lib/python3.9/site-packages/omero/cli.py", line 1381, in execute
    args.func(args)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/cli.py", line 65, in _wrapper
    return func(self, *args, **kwargs)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/cli.py", line 236, in masks
    image_shapes_to_zarr(image, ["Mask"], args)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/masks.py", line 165, in image_shapes_to_zarr
    saver.save(values, name)
  File "/Users/pwalczysko/Work/omero-cli-zarr/src/omero_zarr/masks.py", line 302, in save
    mask_shape,
UnboundLocalError: local variable 'mask_shape' referenced before assignment

@will-moore
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@pwalczysko My output for that was:

$ omero zarr masks Image:5514374 --label-map=5514374.rois
Using session for [email protected]:4064. Idle timeout: 10 min. Current group: Public
Export Masks on Image: cell0005_R0001.companion.ome
Found 2183 mask shapes in 80 ROIs
Label map: Cell (count: 40)
Unique dimensions: {'T': {0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39}, 'C': {None}, 'Z': {1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29}}
source_image 5514374.zarr
Ignoring dimensions {'C'}
Created 5514374.zarr/labels/Cell
Label map: Chromosomes (count: 40)
Unique dimensions: {'T': {0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39}, 'C': {None}, 'Z': {1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25}}
source_image 5514374.zarr
Ignoring dimensions {'C'}
Created 5514374.zarr/labels/Chromosomes

The first line to differ is where you have Label map: Cell (count: 0), so it looks like it's not finding or reading the 5514374.rois file correctly. Can you cat 5514374.rois to check it looks right?

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pwalczysko commented Oct 12, 2021

Sorry, my 5514374.rois was actually 5514375.rois as I did not amend the ID in the hql. Now, with correct 5514374.rois I get

omero zarr masks Image:5514374 --label-map=5514374.rois
Using session for [email protected]:4064. Idle timeout: 10 min. Current group: Public
Export Masks on Image: cell0005_R0001.companion.ome
Found 2183 mask shapes in 80 ROIs
Label map: Cell (count: 40)
Unique dimensions: {'T': {0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39}, 'C': {None}, 'Z': {1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29}}
source_image 5514374.zarr
Ignoring dimensions {'C'}
Created 5514374.zarr/labels/Cell
Label map: Chromosomes (count: 40)
Unique dimensions: {'T': {0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39}, 'C': {None}, 'Z': {1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25}}
source_image 5514374.zarr
Ignoring dimensions {'C'}
Created 5514374.zarr/labels/Chromosomes

Also, it opens and looks fine in napari too.

@sbesson
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sbesson commented Oct 12, 2021

@will-moore https://pypi.org/project/ome-zarr/0.2.0/ has just been released with the new axes API for writing 2D-4D data. Can you update setup.py to indicate the minimal requirement?

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6 participants