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Context menus
Contig
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Reverse – Reverse compliment the contig
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Remove – Remove the contig from the canvas
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Duplicate – Duplicate the contig
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Details – Bring up details about the contigs length, GC content, skew, coverage and edges
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to/fr – Bring up a menu that allows the user to highlight edges. Also allows the user to add, duplicate or move contigs attached to the 3’ end of the selected contig (to) or the 5’ end (fr)
Blast
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Query/Subject
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Move – Move to query/subject so it is aligns with its match
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Goto – Move to the query or subject
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Write – Write the aligned query or subject sequence to a FASTA file
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Remove – Remove the hit
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Details – Show details about the hit such as identity, mismatches etc.
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To back/front – Move the blast hit to the back or front of the canvas
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Highlight – Outline the hit with a specific colour
- Introduction to Contiguity
- Requirements
- Installation
- Citing Contiguity
- Workflow and Examples
- Finishing a PacBio HGAP assembly
- Ordering contigs
- Finding passenger genes
- Identifying plasmid contigs
- Menu overview
- File
- View
- Tools
- Viewing the Assembly
- Canvas overview
- Context menus
- Creating comparisons
- Comparison to a reference
- Self comparison
- Finding paths
- Creating scaffolds
- Creating scaffolds from a CAG
- Creating scaffolds from a PacBio Assembly
- Constructing a Contig adjacency graph
- CAG creation GUI
- CAG creation command-line