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farchaab authored Nov 5, 2024
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```

> [!IMPORTANT]
> [Apptainer](https://apptainer.org/) is the default and recommended dependency deployment method for maximum reproducibility ! If you would like to use conda you can specify `--sdm conda`.
> [Apptainer](https://apptainer.org/) is the default and recommended dependency deployment method for maximum reproducibility !
>
> If you would like to use conda you can specify `--sdm conda`.
#### :card_index_dividers: Outputs

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| :---------- | :------- | :------- | :------ | :---- | :----- | :----- | :------ |
| phage.fq.gz | 1486 | 13203006 | 8884.9 | 12329 | 73.99 | 62.65 | 13.60 |

- PacBio
- PacBio HiFi

```sh
mess run -i phage.tsv -o mess_out/pacbio --tech pacbio --error hifi
Expand All @@ -212,6 +214,9 @@ seqkit stats --all -T -b mess_out/pacbio/fastq/*

Inspired by [readSimulator](https://github.com/wanyuac/readSimulator)'s approach, `mess` can shuffle genome start points to get circular genome assemblies.

> [!WARNING]
> All contigs in the fasta will be circularised
- Linear (default, `--rotate 1`)

```sh
Expand All @@ -232,6 +237,8 @@ mess run -i phage.tsv --rotate 3 -o mess_out/circular
<img src="https://github.com/user-attachments/assets/bab7fbbd-e012-442e-bc27-ba3e893ea673" width=50% height=50%>
</p>


> [!NOTE]
> Assembled using [unicycler](https://github.com/rrwick/Unicycler), visualized using [bandage](https://github.com/rrwick/Bandage)
## :sos: Help
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