CRISPR update #866
CRISPR update #866
Conversation
* 6 pre-prints have now been published; citations have been updated * 1 new paper on an existing approach * 3 new approaches
|
AppVeyor build 1.0.3323 for commit 1a93018 is now complete. Found 9 potential spelling error(s). Preview:content/10.diagnostics.md:134:signallingcontent/10.diagnostics.md:135:crRNA content/10.diagnostics.md:135:thermophilus content/10.diagnostics.md:135:Thermus content/10.diagnostics.md:136:pyrophosphates content/10.diagnostics.md:153:Francisella content/10.diagnostics.md:153:novicida content/10.diagnostics.md:153:ortholog content/20.pharmaceuticals.md:566:overactivation... |
There was a problem hiding this comment.
This technology is seriously amazing! @cbrueffer would you be able to review this text?
|
I'll have a look; not a CRISPR expert though, so maybe someone more qualified should read this as well. |
|
AppVeyor build 1.0.3342 for commit f261236 is now complete. Found 9 potential spelling error(s). Preview:content/10.diagnostics.md:151:signallingcontent/10.diagnostics.md:152:crRNA content/10.diagnostics.md:152:thermophilus content/10.diagnostics.md:152:Thermus content/10.diagnostics.md:153:pyrophosphates content/10.diagnostics.md:170:Francisella content/10.diagnostics.md:170:novicida content/10.diagnostics.md:170:ortholog content/20.pharmaceuticals.md:867:monotherapy... |
|
@nilswellhausen has volunteered to review this text |
|
AppVeyor build 1.0.3416 for commit e4289cc is now complete. Found 31 potential spelling error(s). Preview:content/10.diagnostics.md:151:signallingcontent/10.diagnostics.md:152:crRNA content/10.diagnostics.md:152:thermophilus content/10.diagnostics.md:152:Thermus content/10.diagnostics.md:153:pyrophosphates content/10.diagnostics.md:170:Francisella content/10.diagnostics.md:170:novicida content/10.diagnostics.md:170:ortholog content/20.pharmaceuticals.md:724:AbbVie content/20.pharmaceuticals.md:724:ABVV content/20.pharmaceuti... |
There was a problem hiding this comment.
I am not a CRISPR expert but am going to approve and merge. @nilswellhausen if you get a chance to do a read-through sometime before submission, it would be great to have someone with domain expertise review as well!
| This goal of amplification-free detection was later achieved for a Cas13a-based system [@doi:10.1016/j.cell.2020.12.001]. | ||
| This approach combined multiple CRISPR RNAs to increase Cas13a activation, which is detected by a fluorescent reporter. | ||
| Importantly, because the viral RNA is detected directly, the test yields a quantitative measurement rather than a binary result. | ||
| The study also shows that fluorescence can be measured in a custom-made dark box with a mobile phone camera and a low-cost laser illumination and collection optics. |
There was a problem hiding this comment.
I would leave that part out if the accuracy mentioned below was not achieved by the phone camera approach because that could be misleading.
There was a problem hiding this comment.
These results were achieved using the phone-based setup: "A third key advance in our work is the demonstration that a compact microscope based on a mobile phone and low-cost optics can accurately read the Cas13a direct detection assay, enabling ∼100 copies/μL sensitivity in 30 min of measurement and accurate diagnosis of a set of pre-extracted RNA from patient samples in 5 min of measurement time on the device."
|
AppVeyor build 1.0.3972 for commit 1cb75de is now complete. Found 12 potential spelling error(s). Preview:content/09.evolution.md:83:nonsynonymouscontent/09.evolution.md:139:LVNA content/10.diagnostics.md:151:signalling content/10.diagnostics.md:152:crRNA content/10.diagnostics.md:152:thermophilus content/10.diagnostics.md:152:Thermus content/10.diagnostics.md:153:pyrophosphates content/10.diagnostics.md:170:Francisella content/10.diagnostics.md:170:novicida content/10.diagnostics.md:170:ortholog content/23.vaccines-app.md:1... |
Co-authored-by: nilswellhausen <[email protected]>
|
AppVeyor build 1.0.4743 for commit a19d47f is now complete. Found 11 potential spelling error(s). Preview:content/10.diagnostics.md:163:crRNAcontent/10.diagnostics.md:163:thermophilus content/10.diagnostics.md:163:Thermus content/10.diagnostics.md:164:pyrophosphates content/10.diagnostics.md:181:Francisella content/10.diagnostics.md:181:novicida content/10.diagnostics.md:181:ortholog content/22.vaccines.md:492:AE content/22.vaccines.md:494:AE content/23.vaccines-app.md:15:IgGs content/23.vaccines-app.md:392:IgGs... |
|
@jessegmeyerlab I am merging this in, if you don't mind taking a look! |
[ci skip] This build is based on 43179f5. This commit was created by the following CI build and job: https://github.com/greenelab/covid19-review/commit/43179f550be8f6d7f0d0d61b982cf70793a6e5c4/checks https://github.com/greenelab/covid19-review/runs/1663751555
[ci skip] This build is based on 43179f5. This commit was created by the following CI build and job: https://github.com/greenelab/covid19-review/commit/43179f550be8f6d7f0d0d61b982cf70793a6e5c4/checks https://github.com/greenelab/covid19-review/runs/1663751555
Description of the proposed additions or changes
Related issues
This is the CRISPR update as per #818
Checklist