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adds a barcode option to FastqToBam #936

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merged 5 commits into from
Sep 11, 2023
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Commits on Sep 9, 2023

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Commits on Sep 10, 2023

  1. Revert "minor doc fix, platform unit does not relate to sample barcode"

    This reverts commit b960886.
    
    GATK seems to want a sample-barcode in there too
    
    >PU = Platform Unit
    >The PU holds three types of information, the {FLOWCELL_BARCODE}.{LANE}.{SAMPLE_BARCODE}. The {FLOWCELL_BARCODE} refers to the unique identifier for a particular flow cell. The {LANE} indicates the lane of the flow cell and the {SAMPLE_BARCODE} is a sample/library-specific identifier. Although the PU is not required by GATK but takes precedence over ID for base recalibration if it is present. In the example shown earlier, two read group fields, ID and PU, appropriately differentiate flow cell lane, marked by .2, a factor that contributes to batch effects.
    >(https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups)
    bwlang committed Sep 10, 2023
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Commits on Sep 11, 2023

  1. a list as suggested

    bwlang committed Sep 11, 2023
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