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# Single-Cell In-Situ python package | ||
<div align="center"> | ||
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# **scispy** | ||
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**Single-Cell In-Situ Spatial-Omics Data Analysis** | ||
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--- | ||
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<p align="center"> | ||
<a href="https://scispy.readthedocs.io/en/latest/" target="_blank">Documentation</a> | ||
<a href="https://scispy.readthedocs.io/en/latest/notebooks/tutorial.html" target="_blank">Examples</a> | ||
<a href="https://www.biorxiv.org/" target="_blank">Preprint</a> | ||
</p> | ||
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[![Tests][badge-tests]][link-tests] | ||
[![Documentation][badge-docs]][link-docs] | ||
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[link-tests]: https://github.com/bfxomics/scispy/actions/workflows/test.yml | ||
[badge-docs]: https://img.shields.io/readthedocs/scispy | ||
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scispy is a pthon package for in-situ spatial-omics datasets analysis, mainly developped for vizgen merscope, | ||
scispy is build on top of spatialdata and spatialdata-io and spatialdata-plot librairies which can handle for | ||
Nanostring (cosmx) and 10xGenomics (Xenium) experiments. | ||
</div> | ||
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## Background | ||
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<p> | ||
A pthon package build on top of spatialdata for Single-Cell In-Situ Spatial-Omics data analysis, developped to handle Vizgen (merscope), Nanostring (cosmx) and 10xGenomics (Xenium) experiments. | ||
</p> | ||
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<p align="center"> | ||
<img src="https://github.com/cobioda/scispy/docs/_static/scispy.png" width="500px"> | ||
</p> | ||
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## Features | ||
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- **Read in-situ spatial-omics assays experiments**: build on top of spatialdata package | ||
- **Automatic cell type annotation**: scanvi implementation | ||
- **Import anatomical .csv shape file from xenium explorer**: as anndata observation | ||
- **Automatic run pseudobulk data analysis**: using decoupler and pydeseq2 packages | ||
- **Compute cell type proportion in region**: integrating statistical test in case of replicates | ||
- **Produce high quality spatial figures**: build on top of spatialdata_plot package | ||
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## Getting started | ||
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Please refer to the [documentation][link-docs]. In particular, the | ||
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- [API documentation][link-api]. | ||
- [Tutorials][link-tutorial] | ||
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## Installation | ||
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You need to have Python 3.9 or newer installed on your system. | ||
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<!-- | ||
1) Install the latest release of `scispy` from `PyPI <https://pypi.org/project/scispy/>`_: | ||
```bash | ||
pip install scispy | ||
``` | ||
--> | ||
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Install the latest development version: | ||
1. Create a conda environment (Python >= 3.10) | ||
2. Install scispy using pip: | ||
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```bash | ||
conda create -n scispy python==3.10 | ||
conda activate scispy | ||
pip install git+https://github.com/cobioda/scispy.git@main | ||
``` | ||
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## Release notes | ||
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See the [changelog][changelog]. | ||
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## Contact | ||
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For questions and help requests, you can reach out the main developer of this package: in the [kevin lebrigand](mailto:[email protected]). | ||
If you found a bug, please use the [issue tracker][issue-tracker]. | ||
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## Citation | ||
## Contribution | ||
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> preprint available soon | ||
If you found a bug or you want to propose a new feature, please use the [issue tracker][issue-tracker]. | ||
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[issue-tracker]: https://github.com/cobioda/scispy/issues | ||
[changelog]: https://scispy.readthedocs.io/en/latest/changelog.html | ||
[link-docs]: https://scispy.readthedocs.io | ||
[link-api]: https://scispy.readthedocs.io/en/latest/api.html | ||
[link-tutorial]: https://scispy.readthedocs.io/en/latest/notebooks/tutorial.html |