A non-guided transcriptome assembly usually aims to the de novo assembly method of creating a transcriptome without the aid of a reference genome. The availability of Next Generation Sequencing (NGS) technologies allows researchers to capture the spatial or temporal profile of gene expresion from a huge types of biological samples. The Following markdown describe an integrative workflow analysis in RNA-seq data based on the current bioinformatic methods.
-Ricardo Gore
- Processing Raw libraries .
- Running Trinity .
- Transrating Assembly .
- Annotation .
- Abundance .
- Differential Expression .
- Functional Annotation .
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The Center for Scientific Research and Higher Education of Ensenada (CICESE), Mexico have a computer cluster (Paper) with many bioinformatic apps installed within ready to implement by subscribed users.
Please, email PhD A. Lago in order to request a cluster account (a prior authorization from your responsal)