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Adjust comments in probaln_glocal()
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Adds a comment explaining that the f[] and b[] arrays count
positions from 1, allowing 0 to be used to more easily handle
the edges of the alignment matrix.

Changes the comment explaining the line:
           if (u < 3 || u >= i_dim) continue;
used in some of the loops over f[] and b[].  While it does
prevent overstepping the array boundaries, its main
function is to select the parts over which the scores have
previously been calculated.  A change in 5d7a782 to fix
excess memory usage got the high end slightly wrong (using
i_dim - 3).  When the query sequence length was less than the
band width, this could lead to the last column being
incorrectly missed out from parts of the calculation.
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daviesrob authored and vasudeva8 committed Aug 17, 2023
1 parent 7099640 commit 857112a
Showing 1 changed file with 20 additions and 14 deletions.
34 changes: 20 additions & 14 deletions probaln.c
Original file line number Diff line number Diff line change
Expand Up @@ -140,6 +140,13 @@ int probaln_glocal(const uint8_t *ref, int l_ref, const uint8_t *query,
bM = (1 - c->d) / l_ref; // (bM+bI)*l_ref==1
bI = c->d / l_ref;

// f[] and b[] are 2-d arrays of three scores, with rows along the
// query and columns across the band. The first query base and
// first band position appear at index 1 allowing edge conditions
// to be stored in index 0. Hence the loops below appear to use
// 1-based indexing instead of 0-based as you'd normally expect in C,
// and the sequences are accessed using query[i - 1] and ref[k - 1].

/*** forward ***/
// f[0]
set_u(k, bw, 0, 0);
Expand Down Expand Up @@ -245,20 +252,19 @@ int probaln_glocal(const uint8_t *ref, int l_ref, const uint8_t *query,
{ // f[l_query+1]
double sum;
double M = 1./s[l_query];
// Note this goes up to <= l_ref, meaning we are accessing 1 beyond
// the end of the sequence. However we allocated above with
// (l_query+1)*i_dim (plus appropriate l_ref vs l_query in band width)
// so this should be sufficient.
//
// This fixes Issue #1605 where band width equal to sequence length
// gives incorrect alignments, due to the last value not being filled
// out correctly.
//
// I am unsure why the limit was previously set at u >= i_dim - 3, but
// can only conjecture it was due to forgetting the l_query+1 alloc.
// I am also unsure why "u < 3" is used instead of "u < 0", however
// changing that does change behaviour for common usage (unlike
// "idim - 3" to "idim").
// Note that this goes from 1 to l_ref inclusive, but as the
// alignment is banded not all of the values will have been
// calculated (the rest are taken as 0), so the summation
// actually goes over the values set in the last iteration of
// the previous loop (when i = l_query). For some reason lost to
// time this is done by looking for valid values of 'u' instead of
// working out 'beg' and 'end'.

// From HTSlib 1.8 to 1.17, the endpoint was incorrectly set
// to i_dim - 3. When l_query <= bandwidth, this caused the last
// column to be missed, and if l_ref == l_query then a match at the end
// could incorrectly be reported as an insertion. See #1605.

for (k = 1, sum = 0.; k <= l_ref; ++k) {
int u;
set_u(u, bw, l_query, k);
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