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Autocycler trim
Ryan Wick edited this page Sep 4, 2024
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Usage: autocycler trim [OPTIONS] --cluster_dir <CLUSTER_DIR>
Options:
-c, --cluster_dir <CLUSTER_DIR> Autocycler cluster directory containing 1_untrimmed.gfa file
(required)
--min_identity <MIN_IDENTITY> Minimum alignment identity for trimming alignments [default: 0.75]
--max_unitigs <MAX_UNITIGS> Maximum unitigs used for overlap alignment, set to 0 to disable
trimming [default: 5000]
--mad <MAD> Allowed variability in cluster length, measured in median absolute
deviations, set to 0 to disable exclusion of length outliers
[default: 5.0]
-t, --threads <THREADS> Number of CPU threads [default: 8]
-h, --help Print help
-V, --version Print version
- Step 1: Autocycler subsample
- Step 2: Generating input assemblies
- Step 3: Autocycler compress
- Step 4: Autocycler cluster
- Step 5: Autocycler trim
- Step 6: Autocycler resolve
- Step 7: Autocycler combine