-
Notifications
You must be signed in to change notification settings - Fork 6
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
Merge pull request #6 from tomdstanton/patch-1
Create README.md
- Loading branch information
Showing
1 changed file
with
33 additions
and
0 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,33 @@ | ||
# autoautocycler | ||
|
||
`autoautocycler` is a simple shell wrapper around [Autocycler](https://github.com/rrwick/Autocycler.git) for running all steps on multiple assemblies with a single command. | ||
|
||
## Installation | ||
No installation required! Just make sure [Autocycler](https://github.com/rrwick/Autocycler.git) is installed and make the script excecutable with | ||
`chmod +x autoautocycler.sh`. | ||
|
||
## Usage | ||
Running `./autoautocycler.sh` with `-h`/`--help` will print all options: | ||
|
||
```sh | ||
Usage: autoautocycler <reads> [<reads> ...] [options] | ||
<reads> Long reads to assemble in fastq(.gz) format | ||
-o, --out Output directory (default: .) | ||
-t, --threads #threads to use (default: all available) | ||
-c, --count #subsampled read sets to output (default: 4) | ||
-k, --kmer K-mer size for De Bruijn graph (default: 51) | ||
-s, --size Genome size (default: AUTO) | ||
-a, --assemblers Assemblers to use (default: all available) | ||
Possible assemblers: canu flye lja metamdbg miniasm necat nextdenovo raven redbean | ||
Note: this argument MUST BE WRAPPED in quotes | ||
``` | ||
|
||
E.g. for making assemblies with flye, miniasm and raven, just run: | ||
```sh | ||
./autoautocycler.sh -o autocycler -c 2 -s 5500000 -a "flye miniasm raven" reads/*.fastq.gz | ||
``` | ||
Here I've set the subsampling count to 2 and set the genome size to 5500000 to skip the automatic genome size calculation with raven. | ||
|
||
## Bug reporting | ||
Bugs can be reported using the [Autocycler issues page](https://github.com/rrwick/Autocycler/issues) on GitHub, | ||
just make sure you tag me with @tomdstanton. |