Stranded Workflow
Pre-release
Pre-release
What's new in 0.19.1
New Features
seismic wf
now accepts--sep-strands
. This change is possible becauseseismic relate
now also accepts--sep-strands
and, if that option is given, will auto-generate a FASTA file of both strands. Thus, you can also runseismic align
andseismic relate
separately, as long as you pass the same FASTA file and the same--sep-stands
and--minus-label
options to both commands.seismic +splitbam
is a new command that accepts one or more BAM (or CRAM) files and splits each file into one BAM file for each reference, just like the last phase ofseismic align
. It also accepts the--sep-strands
option if you want to split each reference further into plus and minus strands. This feature is useful when you generate or obtain a BAM file outside of SEISMIC-RNA that contains multiple references, and you need to split it into one file for each reference so that you can pass those files intoseismic relate
orseismic wf
.
Removed Features
seismic align
with--sep-strands
no longer generates a FASTA file of both strands because it is no longer necessary due to the first new feature (above).
Bug Fixes
- Fixed bug where an empty directory called
align-xxxxxxxx
would appear in a sample's output directory if thealign
output directory did not yet exist.
Full Changelog: v0.19.0...v0.19.1