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Add --fold to wf command in amplicon tutorial
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matthewfallan committed Aug 14, 2024
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3 changes: 2 additions & 1 deletion src/userdocs/tutorials/amplicon/index.rst
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Expand Up @@ -332,7 +332,7 @@ Now that the replicates are pooled, the overall coverage will be higher, and so
clustering is more likely to detect true alternative structures.
Process the pooled sample, including with clustering, by running this command::

seismic -v wf --mask-pos rre 176 -p rre GGAGCTTTGTTCCTTGGGTTCTTGG GGAGCTGTTGATCCTTTAGGTATCTTTC --cluster hiv-rre.fa out/dms-pool/relate
seismic -v wf --mask-pos rre 176 -p rre GGAGCTTTGTTCCTTGGGTTCTTGG GGAGCTGTTGATCCTTTAGGTATCTTTC --cluster --fold hiv-rre.fa out/dms-pool/relate

This is what each of the arguments does:

Expand All @@ -345,6 +345,7 @@ This is what each of the arguments does:
section of the reference ``rre`` that corresponds to the amplicon flanked by
primers ``GGAGCTTTGTTCCTTGGGTTCTTGG`` and ``GGAGCTGTTGATCCTTTAGGTATCTTTC``.
- ``--cluster`` means enable clustering to find alternative structures.
- ``--fold`` means enable secondary structure prediction.
- ``hiv-rre.fa`` means use the sequence in this FASTA file as the reference
(i.e. mutation-free) sequence for the RNA.
- ``out/dms-pool/relate`` means search inside ``out/dms-pool/relate`` for data
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