Skip to content

Commit

Permalink
Update example files for tutorial
Browse files Browse the repository at this point in the history
  • Loading branch information
matthewfallan committed Aug 2, 2024
1 parent a8893f9 commit 4593541
Show file tree
Hide file tree
Showing 7 changed files with 4 additions and 4 deletions.
Binary file modified src/userdocs/tutorials/amplicon/data/fq/dms1/dms1_R1.fq.gz
Binary file not shown.
Binary file modified src/userdocs/tutorials/amplicon/data/fq/dms1/dms1_R2.fq.gz
Binary file not shown.
Binary file modified src/userdocs/tutorials/amplicon/data/fq/dms2/dms2_R1.fq.gz
Binary file not shown.
Binary file modified src/userdocs/tutorials/amplicon/data/fq/dms2/dms2_R2.fq.gz
Binary file not shown.
Binary file modified src/userdocs/tutorials/amplicon/data/fq/nodms/nodms_R1.fq.gz
Binary file not shown.
Binary file modified src/userdocs/tutorials/amplicon/data/fq/nodms/nodms_R2.fq.gz
Binary file not shown.
8 changes: 4 additions & 4 deletions src/userdocs/tutorials/amplicon/index.rst
Original file line number Diff line number Diff line change
Expand Up @@ -210,7 +210,7 @@ Run the workflow on both DMS-treated replicates

Process the DMS-treated samples through the whole workflow with this command::

seismic -v wf -x fq/dms1 -x fq/dms2 --mask-pos rre 176 -p rre CAATGACGCTGACGGTACAGG ATCTTTCCACAGCCAGGATTC hiv-rre.fa
seismic -v wf -x fq/dms1 -x fq/dms2 --mask-pos rre 176 -p rre GGAGCTTTGTTCCTTGGGTTCTTGG GGAGCTGTTGATCCTTTAGGTATCTTTC hiv-rre.fa

This is what each of the arguments does:

Expand All @@ -223,9 +223,9 @@ This is what each of the arguments does:
paired-end reads with mate 1 and mate 2 in separate files.
- ``--mask-pos rre 176`` means mask position 176 (because it had a high mutation
rate in the no-DMS sample).
- ``-p rre CAATGACGCTGACGGTACAGG ATCTTTCCACAGCCAGGATTC`` defines a section of
the reference ``rre`` that corresponds to the amplicon flanked by the primers
``CAATGACGCTGACGGTACAGG`` (forward) and ``ATCTTTCCACAGCCAGGATTC`` (reverse).
- ``-p rre GGAGCTTTGTTCCTTGGGTTCTTGG GGAGCTGTTGATCCTTTAGGTATCTTTC`` defines a
section of the reference ``rre`` that corresponds to the amplicon flanked by
primers ``GGAGCTTTGTTCCTTGGGTTCTTGG`` and ``GGAGCTGTTGATCCTTTAGGTATCTTTC``.
- ``hiv-rre.fa`` means use the sequence in this FASTA file as the reference
(i.e. mutation-free) sequence for the RNA.

Expand Down

0 comments on commit 4593541

Please sign in to comment.