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Automating 3 Steps: Qaulity Check - Trimming - Qualiting Check of NGS Raw Data (FASTQ) of large data sets

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QTQ

Commands to use:

  cd QTQ

  chmod +x ./*.sh

 ./qc-trim-qc.sh

  cd ~/Desktop

  mkdir GenopicsApps

  cd GenomicsApps

Download Fastp and Trimmomatic executables and keep inside GenomicsApps.

  get http://opengene.org/fastp/fastp

  chmod a+x ./fastp

or download specified version, i.e. fastp v0.23.4

  wget http://opengene.org/fastp/fastp.0.23.4

  mv fastp.0.23.4 fastp

  chmod a+x ./fastp

Download Trimmomatic

  wget http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic/Trimmomatic-0.39.zip

  unzip Trimmomatic-0.39.zip

  rm Trimmomatic-0.39.zip

Run: I: Input path [String]

O: Output path [String]

L: Minimum length of reads to keep [Int]

T: fp (Fastp) or Tr (Trimmomatic)

  ./qc-trim-qc.sh -I  /path_to_input_data_directory/ -O /path_to_Output_directory/ -L 35 -T fp

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