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Make rlog transformation optional #99

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SusiJo opened this issue Feb 3, 2022 · 2 comments
Closed

Make rlog transformation optional #99

SusiJo opened this issue Feb 3, 2022 · 2 comments

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@SusiJo
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SusiJo commented Feb 3, 2022

Hi there,

when > 50 samples are used for differential gene expression analysis, the rlog transformation becomes very time consuming.
Currently, the transformed table is only used for saving and one quality control plot.
Therefore my suggestion would be to add an optional parameter --rlog [bool] that defines whether the transformation should be made or not. This would save much time when performing the analysis for a large number of samples.

@ggabernet ggabernet added this to the v2.0.0 milestone Feb 3, 2022
@SusiJo
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SusiJo commented Feb 4, 2022

Concerning the rlog in general, the RNAseq_Report.md needs to be updated because actually it's not the rlog transformation that is used for the PCA plots, but the vst transformation (see these lines: report-305, report-309, DESeq2.R-459 and DESeq2.R-472

@WackerO
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WackerO commented May 24, 2022

Closing this as it was addressed in #107 and #110

@WackerO WackerO closed this as completed May 24, 2022
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