bioformats2raw.layout

0.4/examples/bf2raw/.config.json

@@ -0,0 +1,3 @@
+{
+    "schema": "schemas/bf2raw.schema"
+}

0.4/examples/bf2raw/image.json

@@ -0,0 +1,3 @@
+{
+  "bioformats2raw.layout" : 3
+}

0.4/examples/bf2raw/plate.json

@@ -0,0 +1,22 @@
+{
+  "bioformats2raw.layout" : 3,
+  "plate" : {
+    "columns" : [ {
+      "name" : "1"
+    } ],
+    "name" : "Plate Name 0",
+    "wells" : [ {
+      "path" : "A/1",
+      "rowIndex" : 0,
+      "columnIndex" : 0
+    } ],
+    "field_count" : 1,
+    "rows" : [ {
+      "name" : "A"
+    } ],
+    "acquisitions" : [ {
+      "id" : 0
+    } ],
+    "version" : "0.4"
+  }
+}

0.4/examples/ome/.config.json

@@ -0,0 +1,3 @@
+{
+    "schema": "schemas/ome.schema"
+}

0.4/examples/ome/series-2.json

@@ -0,0 +1,3 @@
+{
+  "series" : [ "0", "1" ]
+}

0.4/index.bs

@@ -99,6 +99,14 @@ The key words “MUST”, “MUST NOT”, “REQUIRED”, “SHALL”, “SHALL
 “RECOMMENDED”, “MAY”, and “OPTIONAL” are to be interpreted as described in
 [RFC 2119](https://tools.ietf.org/html/rfc2119).
 
+<p>
+<dfn>Transitional</dfn> metadata is added to the specification with the
+intention of removing it in the future. Implementations may be expected (MUST) or
+encouraged (SHOULD) to support the reading of the data, but writing will usually
+be optional (MAY). Examples of transitional metadata include custom additions by
+implementations that are later submitted as a formal specification. (See [[#bf2raw]])
+</p>
+
 Some of the JSON examples in this document include commments. However, these are only for
 clarity purposes and comments MUST NOT be included in JSON objects.
 
@@ -240,6 +248,85 @@ keys as specified below for discovering certain types of data, especially images
 
 If part of [[#multiscale-md]], the length of "axes" MUST be equal to the number of dimensions of the arrays that contain the image data.
 
+"bioformats2raw.layout" (transitional) {#bf2raw}
+------------------------------------------------
+
+[=Transitional=] "bioformats2raw.layout" metadata identifies a group which implicitly describes a series of images.
+The need for the collection stems from the common "multi-image file" scenario in microscopy. Parsers like Bio-Formats
+define a strict, stable ordering of the images in a single container that can be used to refer to them by other tools.
+
+In order to capture that information within an OME-NGFF dataset, `bioformats2raw` internally introduced a wrapping layer.
+The bioformats2raw layout has been added to v0.4 as a transitional specification to specify filesets that already exist
+in the wild. An upcoming NGFF specification will replace this layout with explicit metadata.
+
+<h4 id="bf2raw-layout" class="no-toc">Layout</h4>
+
+Typical Zarr layout produced by running `bioformats2raw` on a fileset that contains more than one image (series > 1):
+
+<pre>
+series.ome.zarr               # One converted fileset from bioformats2raw
+    ├── .zgroup
+    ├── .zattrs               # Contains "bioformats2raw.layout" metadata
+    ├── OME                   # Special group for containing OME metadata
+    │   ├── .zgroup
+    │   ├── .zattrs           # Contains "series" metadata
+    │   └── METADATA.ome.xml  # OME-XML file stored within the Zarr fileset
+    ├── 0                     # First image in the collection
+    ├── 1                     # Second image in the collection
+    └── ...
+</pre>
+
+<h4 id="bf2raw-attributes" class="no-toc">Attributes</h4>
+
+The top-level `.zattrs` file must contain the `bioformats2raw.layout` key:
+<pre class=include-code>
+path: examples/bf2raw/image.json
+highlight: json
+</pre>
+
+If the top-level group represents a plate, the `bioformats2raw.layout` metadata will be present but
+the "plate" key MUST also be present, takes precedence and parsing of such datasets should follow [[#plate-md]]. It is not
+possible to mix collections of images with plates at present.
+
+<pre class=include-code>
+path: examples/bf2raw/plate.json
+highlight: json
+</pre>
+
+The `.zattrs` file within the OME group may contain the "series" key:
+
+<pre class=include-code>
+path: examples/ome/series-2.json
+highlight: json
+</pre>
+
+<h4 id="bf2raw-details" class="no-toc">Details</h4>
+
+Conforming groups:
+
+- MUST have the value "3" for the "bioformats2raw.layout" key in their `.zattrs` metadata at the top of the hierarchy;
+- SHOULD have OME metadata representing the entire collection of images in a file named "OME/METADATA.ome.xml" which:
+    - MUST adhere to the OME-XML specification but
+    - MUST use `<MetadataOnly/>` elements as opposed to `<BinData/>`, `<BinaryOnly/>` or `<TiffData/>`;
+    - MAY make use of the [minimum specification](https://docs.openmicroscopy.org/ome-model/6.2.2/specifications/minimum.html).
+
+Additionally, the logic for finding the Zarr group for each image follows the following logic:
+
+- If "plate" metadata is present, images MUST be located at the defined location.
+    - Matching "series" metadata as described next MAY be provided for tools that are unaware of the "plate" specification.
+- If the "OME" Zarr group exists, it:
+    - MAY contain a "series" attribute. If so:
+        - "series" MUST be a list of string objects, each of which is a path to an image group.
+        - The order of the paths MUST match the order of the "Image" elements in "OME/METADATA.ome.xml" if provided.
+- If the "series" attribute does not exist and no "plate" is present:
+    - separate "multiscales" images MUST be stored in consecutively numbered groups starting from 0 (i.e. "0/", "1/", "2/", "3/", ...).
+- Every "multiscales" group MUST represent exactly one OME-XML "Image" in the same order as either the series index or the group numbers.
+
+Conforming readers:
+- SHOULD make users aware of the presence of more than one image (i.e. SHOULD NOT default to only opening the first image);
+- MAY use the "series" attribute in the "OME" group to determine a list of valid groups to display;
+- MAY choose to show all images within the collection or offer the user a choice of images, as with <dfn export="true"><abbr title="High-content screening">HCS</abbr></dfn> plates;
+- MAY ignore other groups or arrays under the root of the hierarchy.
 
 "coordinateTransformations" metadata {#trafo-md}
 -------------------------------------

0.4/schemas/bf2raw.schema

@@ -0,0 +1,14 @@
+{
+  "$schema": "https://json-schema.org/draft/2020-12/schema",
+  "$id": "https://ngff.openmicroscopy.org/latest/schemas/bf2raw.schema",
+  "title": "NGFF container produced by bioformats2raw",
+  "description": "JSON from OME-NGFF .zattrs",
+  "type": "object",
+  "properties": {
+    "bioformats2raw.layout": {
+      "description": "The top-level identifier metadata added by bioformats2raw",
+      "type": "number",
+      "enum": [3]
+    }
+  }
+}

0.4/schemas/ome.schema

@@ -0,0 +1,17 @@
+{
+  "$schema": "https://json-schema.org/draft/2020-12/schema",
+  "$id": "https://ngff.openmicroscopy.org/latest/schemas/ome.schema",
+  "title": "NGFF group produced by bioformats2raw to contain OME metadata",
+  "description": "JSON from OME-NGFF OME/.zattrs linked to an OME-XML file",
+  "type": "object",
+  "properties": {
+    "series": {
+      "description": "An array of the same length and the same order as the images defined in the OME-XML",
+      "type": "array",
+      "items": {
+        "type": "string"
+      },
+      "minContains": 1
+    }
+  }
+}

latest/examples/bf2raw/.config.json

@@ -0,0 +1,3 @@
+{
+    "schema": "schemas/bf2raw.schema"
+}

latest/examples/bf2raw/image.json

@@ -0,0 +1,3 @@
+{
+  "bioformats2raw.layout" : 3
+}

latest/examples/bf2raw/plate.json

@@ -0,0 +1,22 @@
+{
+  "bioformats2raw.layout" : 3,
+  "plate" : {
+    "columns" : [ {
+      "name" : "1"
+    } ],
+    "name" : "Plate Name 0",
+    "wells" : [ {
+      "path" : "A/1",
+      "rowIndex" : 0,
+      "columnIndex" : 0
+    } ],
+    "field_count" : 1,
+    "rows" : [ {
+      "name" : "A"
+    } ],
+    "acquisitions" : [ {
+      "id" : 0
+    } ],
+    "version" : "0.4"
+  }
+}

latest/examples/ome/.config.json

@@ -0,0 +1,3 @@
+{
+    "schema": "schemas/ome.schema"
+}

latest/examples/ome/series-2.json

@@ -0,0 +1,3 @@
+{
+  "series" : [ "0", "1" ]
+}

latest/index.bs

@@ -101,6 +101,14 @@ The key words “MUST”, “MUST NOT”, “REQUIRED”, “SHALL”, “SHALL
 “RECOMMENDED”, “MAY”, and “OPTIONAL” are to be interpreted as described in
 [RFC 2119](https://tools.ietf.org/html/rfc2119).
 
+<p>
+<dfn>Transitional</dfn> metadata is added to the specification with the
+intention of removing it in the future. Implementations may be expected (MUST) or
+encouraged (SHOULD) to support the reading of the data, but writing will usually
+be optional (MAY). Examples of transitional metadata include custom additions by
+implementations that are later submitted as a formal specification. (See [[#bf2raw]])
+</p>
+
 Some of the JSON examples in this document include commments. However, these are only for
 clarity purposes and comments MUST NOT be included in JSON objects.
 
@@ -242,9 +250,89 @@ keys as specified below for discovering certain types of data, especially images
 
 If part of [[#multiscale-md]], the length of "axes" MUST be equal to the number of dimensions of the arrays that contain the image data.
 
+"bioformats2raw.layout" (transitional) {#bf2raw}
+------------------------------------------------
+
+[=Transitional=] "bioformats2raw.layout" metadata identifies a group which implicitly describes a series of images.
+The need for the collection stems from the common "multi-image file" scenario in microscopy. Parsers like Bio-Formats
+define a strict, stable ordering of the images in a single container that can be used to refer to them by other tools.
+
+In order to capture that information within an OME-NGFF dataset, `bioformats2raw` internally introduced a wrapping layer.
+The bioformats2raw layout has been added to v0.4 as a transitional specification to specify filesets that already exist
+in the wild. An upcoming NGFF specification will replace this layout with explicit metadata.
+
+<h4 id="bf2raw-layout" class="no-toc">Layout</h4>
+
+Typical Zarr layout produced by running `bioformats2raw` on a fileset that contains more than one image (series > 1):
+
+<pre>
+series.ome.zarr               # One converted fileset from bioformats2raw
+    ├── .zgroup
+    ├── .zattrs               # Contains "bioformats2raw.layout" metadata
+    ├── OME                   # Special group for containing OME metadata
+    │   ├── .zgroup
+    │   ├── .zattrs           # Contains "series" metadata
+    │   └── METADATA.ome.xml  # OME-XML file stored within the Zarr fileset
+    ├── 0                     # First image in the collection
+    ├── 1                     # Second image in the collection
+    └── ...
+</pre>
+
+<h4 id="bf2raw-attributes" class="no-toc">Attributes</h4>
+
+The top-level `.zattrs` file must contain the `bioformats2raw.layout` key:
+<pre class=include-code>
+path: examples/bf2raw/image.json
+highlight: json
+</pre>
+
+If the top-level group represents a plate, the `bioformats2raw.layout` metadata will be present but
+the "plate" key MUST also be present, takes precedence and parsing of such datasets should follow [[#plate-md]]. It is not
+possible to mix collections of images with plates at present.
+
+<pre class=include-code>
+path: examples/bf2raw/plate.json
+highlight: json
+</pre>
+
+The `.zattrs` file within the OME group may contain the "series" key:
+
+<pre class=include-code>
+path: examples/ome/series-2.json
+highlight: json
+</pre>
+
+<h4 id="bf2raw-details" class="no-toc">Details</h4>
+
+Conforming groups:
+
+- MUST have the value "3" for the "bioformats2raw.layout" key in their `.zattrs` metadata at the top of the hierarchy;
+- SHOULD have OME metadata representing the entire collection of images in a file named "OME/METADATA.ome.xml" which:
+    - MUST adhere to the OME-XML specification but
+    - MUST use `<MetadataOnly/>` elements as opposed to `<BinData/>`, `<BinaryOnly/>` or `<TiffData/>`;
+    - MAY make use of the [minimum specification](https://docs.openmicroscopy.org/ome-model/6.2.2/specifications/minimum.html).
+
+Additionally, the logic for finding the Zarr group for each image follows the following logic:
+
+- If "plate" metadata is present, images MUST be located at the defined location.
+    - Matching "series" metadata as described next MAY be provided for tools that are unaware of the "plate" specification.
+- If the "OME" Zarr group exists, it:
+    - MAY contain a "series" attribute. If so:
+        - "series" MUST be a list of string objects, each of which is a path to an image group.
+        - The order of the paths MUST match the order of the "Image" elements in "OME/METADATA.ome.xml" if provided.
+- If the "series" attribute does not exist and no "plate" is present:
+    - separate "multiscales" images MUST be stored in consecutively numbered groups starting from 0 (i.e. "0/", "1/", "2/", "3/", ...).
+- Every "multiscales" group MUST represent exactly one OME-XML "Image" in the same order as either the series index or the group numbers.
+
+Conforming readers:
+- SHOULD make users aware of the presence of more than one image (i.e. SHOULD NOT default to only opening the first image);
+- MAY use the "series" attribute in the "OME" group to determine a list of valid groups to display;
+- MAY choose to show all images within the collection or offer the user a choice of images, as with <dfn export="true"><abbr title="High-content screening">HCS</abbr></dfn> plates;
+- MAY ignore other groups or arrays under the root of the hierarchy.
+
 
 "coordinateTransformations" metadata {#trafo-md}
--------------------------------------
+------------------------------------------------
 
 "coordinateTransformations" describe a series of transformations that map between two coordinate spaces (defined by "axes").
 For example, to map a discrete data space of an array to the corresponding physical space.

latest/schemas/bf2raw.schema

@@ -0,0 +1,14 @@
+{
+  "$schema": "https://json-schema.org/draft/2020-12/schema",
+  "$id": "https://ngff.openmicroscopy.org/latest/schemas/bf2raw.schema",
+  "title": "NGFF container produced by bioformats2raw",
+  "description": "JSON from OME-NGFF .zattrs",
+  "type": "object",
+  "properties": {
+    "bioformats2raw.layout": {
+      "description": "The top-level identifier metadata added by bioformats2raw",
+      "type": "number",
+      "enum": [3]
+    }
+  }
+}

latest/schemas/ome.schema

@@ -0,0 +1,17 @@
+{
+  "$schema": "https://json-schema.org/draft/2020-12/schema",
+  "$id": "https://ngff.openmicroscopy.org/latest/schemas/ome.schema",
+  "title": "NGFF group produced by bioformats2raw to contain OME metadata",
+  "description": "JSON from OME-NGFF OME/.zattrs linked to an OME-XML file",
+  "type": "object",
+  "properties": {
+    "series": {
+      "description": "An array of the same length and the same order as the images defined in the OME-XML",
+      "type": "array",
+      "items": {
+        "type": "string"
+      },
+      "minContains": 1
+    }
+  }
+}

latest/tests/test_validation.py

@@ -89,3 +89,17 @@ def test_run(suite):
     resolver = RefResolver.from_schema(suite.schema, store=schema_store)
     validator = Validator(suite.schema, resolver=resolver)
     suite.validate(validator)
+
+
+def test_example_configs():
+    """
+    Test that all example folders have a config file
+    """
+    missing = []
+    for subdir in os.walk("examples"):
+        has_examples = glob.glob(f"{subdir[0]}/*.json")
+        has_config = glob.glob(f"{subdir[0]}/.config.json")
+        if has_examples and not has_config:
+            missing.append(subdir[0])
+    if missing:
+        raise Exception(f"Directories missing configs: {missing}")