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@@ -48,13 +48,13 @@ Due to its pure local-assembly strategy, Lancet currently has longer runtimes co | |
``` | ||
$NUMBER_OF_AUTOSOMES=22 | ||
for chrom in `seq 1 $NUMBER_OF_AUTOSOMES` X Y; do | ||
qsub | ||
qsub \ | ||
-N lancet_chr${chrom} \ | ||
-cwd \ | ||
-pe smp 8 \ | ||
-q dev.q \ | ||
-j y \ | ||
-b y \ | ||
-cwd \ | ||
-pe smp 8 \ | ||
-q dev.q \ | ||
-j y \ | ||
-b y \ | ||
"lancet --tumor T.bam --normal N.bam --ref ref.fa --reg $chrom --num-threads 8 > ${chrom}.vcf" | ||
// merge VCF files | ||
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@@ -142,7 +142,7 @@ The final graph (after compression) containing one single variant is depicted be | |
_____|\__,_|_| _|\___|\___|\__| | ||
Program: lancet (micro-assembly somatic variant caller) | ||
Version: 1.0.0 (beta), September 18 2016 | ||
Version: 1.0.2 (beta), Ocotber 17 2017 | ||
Contact: Giuseppe Narzisi <[email protected]> | ||
Usage: lancet [options] --tumor <BAM file> --normal <BAM file> --ref <FASTA file> --reg <chr:start-end> | ||
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@@ -157,7 +157,7 @@ Required | |
Optional | ||
--min-k, k <int> : min kmersize [default: 11] | ||
--max-k, -K <int> : max kmersize [default: 100] | ||
--max-k, -K <int> : max kmersize [default: 101] | ||
--trim-lowqual, -q <int> : trim bases below qv at 5' and 3' [default: 10] | ||
--min-base-qual, -C <int> : minimum base quality required to consider a base for SNV calling [default: 17] | ||
--quality-range, -Q <char> : quality value range [default: !] | ||
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