Merge pull request #1752 from FriederikeHanssen/fullsize #6945
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Dec 11, 2024 in 0s
9 tests run, 8 passed, 0 skipped, 1 failed.
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Check failure on line 1 in Test pipeline
github-actions / JUnit Test Report
Test pipeline.Run with profile test | --tools strelka --no_intervals | somatic
Assertion failed:
2 of 2 assertions failed
Raw output
Nextflow stdout:
N E X T F L O W ~ version 24.11.0-edge
Launching `/home/runner/work/sarek/sarek/tests/../main.nf` [agitated_tesla] DSL2 - revision: 3954909713
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[0;32m,--.[0;30m/[0;32m,-.[0m
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[0;37m \ [0;32m| \[0m /[0m [0;34m.__| /¯¯\ | \ |___ | \[0m
[0;37m `[0;32m|[0m____[0;32m\[0m´[0m
[0;35m nf-core/sarek 3.5.0[0m
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[1mInput/output options[0m
[0;34minput : [0;32m/home/runner/work/sarek/sarek/tests/csv/3.0/recalibrated_somatic.csv[0m
[0;34mstep : [0;32mvariant_calling[0m
[0;34moutdir : [0;32m/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/output[0m
[1mMain options[0m
[0;34msplit_fastq : [0;32m0[0m
[0;34mintervals : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed[0m
[0;34mno_intervals : [0;32mtrue[0m
[0;34mtools : [0;32mstrelka[0m
[1mAnnotation[0m
[0;34mbcftools_annotations : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/sarscov2/illumina/vcf/test2.vcf.gz[0m
[0;34mbcftools_annotations_tbi : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/sarscov2/illumina/vcf/test2.vcf.gz.tbi[0m
[0;34mbcftools_header_lines : [0;32m/home/runner/work/sarek/sarek/tests/config/bcfann_test_header.txt[0m
[1mGeneral reference genome options[0m
[0;34migenomes_base : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data/[0m
[1mReference genome options[0m
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[0;34mdbsnp : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz[0m
[0;34mdbsnp_tbi : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi[0m
[0;34mdbsnp_vqsr : [0;32m--resource:dbsnp,known=false,training=true,truth=false,prior=2.0 dbsnp_146.hg38.vcf.gz[0m
[0;34mdict : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/homo_sapiens/genome/genome.dict[0m
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[0;34mgermline_resource_tbi : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz.tbi[0m
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[0;34mknown_indels_tbi : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi[0m
[0;34mknown_indels_vqsr : [0;32m--resource:mills,known=false,training=true,truth=true,prior=10.0 mills_and_1000G.indels.vcf.gz[0m
[0;34mngscheckmate_bed : [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data//genomics/homo_sapiens/genome/chr21/germlineresources/SNP_GRCh38_hg38_wChr.bed[0m
[0;34msentieon_dnascope_model : [0;32ms3://ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Annotation/Sentieon/SentieonDNAscopeModel1.1.model[0m
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[0;34msnpeff_db : [0;32mWBcel235.105[0m
[0;34mvep_cache : [0;32mnull[0m
[0;34mvep_cache_version : [0;32m113[0m
[0;34mvep_genome : [0;32mWBcel235[0m
[0;34mvep_species : [0;32mcaenorhabditis_elegans[0m
[1mInstitutional config options[0m
[0;34mconfig_profile_name : [0;32mTest profile[0m
[0;34mconfig_profile_description: [0;32mMinimal test dataset to check pipeline function[0m
[0;34mmodules_testdata_base_path: [0;32mhttps://raw.githubusercontent.com/nf-core/test-datasets/modules/data/[0m
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[0;34mconfigFiles : [0;32m[0m
!! Only displaying parameters that differ from the pipeline defaults !!
-[2m----------------------------------------------------[0m-
* The pipeline
https://doi.org/10.12688/f1000research.16665.2
https://doi.org/10.1093/nargab/lqae031
https://doi.org/10.5281/zenodo.3476425
* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/nf-core/sarek/blob/master/CITATIONS.md
Creating env using conda: /home/runner/work/sarek/sarek/./subworkflows/local/prepare_intervals/../../../modules/nf-core/tabix/bgziptabix/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-5a9ba0ebb7bcf86c-497b0f18c811c5671b5e17e0034188ed]
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../subworkflows/local/bam_variant_calling_germline_all/../bam_variant_calling_single_strelka/../../../modules/nf-core/strelka/germline/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-90ad7ffecab6b5bf-438a505d7b44a08705c858f9ad6e02ce]
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../subworkflows/local/cram_sampleqc/../../../subworkflows/local/cram_qc_mosdepth_samtools/../../../modules/nf-core/mosdepth/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-c5b497d07713b814-57cd7e91fe23a8ceb488416c37dae89b]
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../subworkflows/local/cram_sampleqc/../../../subworkflows/local/cram_qc_mosdepth_samtools/../../../modules/nf-core/samtools/stats/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-1abf3db016bf3fb9-873a3583ebab040412cb72c07ed757ab]
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../subworkflows/local/bam_variant_calling_somatic_all/../bam_variant_calling_somatic_strelka/../../../modules/nf-core/strelka/somatic/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce]
[2c/b85c5c] Submitted process > NFCORE_SAREK:PREPARE_INTERVALS:TABIX_BGZIPTABIX_INTERVAL_COMBINED (no_intervals)
[4a/94ccb9] Submitted process > NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)
[c9/ea1f7b] Submitted process > NFCORE_SAREK:SAREK:CRAM_SAMPLEQC:CRAM_QC_RECAL:SAMTOOLS_STATS (sample3)
[4a/94ccb9] NOTE: Process `NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)` terminated with an error exit status (1) -- Execution is retried (1)
[cb/db2a94] Submitted process > NFCORE_SAREK:SAREK:CRAM_SAMPLEQC:CRAM_QC_RECAL:SAMTOOLS_STATS (sample4)
[77/06d45a] Submitted process > NFCORE_SAREK:SAREK:CRAM_SAMPLEQC:CRAM_QC_RECAL:MOSDEPTH (sample3)
[54/1ccc58] Submitted process > NFCORE_SAREK:SAREK:CRAM_SAMPLEQC:CRAM_QC_RECAL:MOSDEPTH (sample4)
[fd/50d7d6] Re-submitted process > NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)
[fd/50d7d6] NOTE: Process `NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)` terminated with an error exit status (1) -- Execution is retried (2)
[d9/38184d] Submitted process > NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_GERMLINE_ALL:BAM_VARIANT_CALLING_SINGLE_STRELKA:STRELKA_SINGLE (sample3)
[83/bba38d] Re-submitted process > NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../subworkflows/local/vcf_qc_bcftools_vcftools/../../../modules/nf-core/bcftools/stats/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-d14f40c429aefa2b-dc6f2c13cbbb0f956fd0966de87d7df8]
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../subworkflows/local/vcf_qc_bcftools_vcftools/../../../modules/nf-core/vcftools/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-ac437bb99679f3c6-ede778c78582be405557f3c8ca0e9927]
[ab/9f098f] Submitted process > NFCORE_SAREK:SAREK:VCF_QC_BCFTOOLS_VCFTOOLS:BCFTOOLS_STATS (sample3)
ERROR ~ Error executing process > 'NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)'
Caused by:
Process `NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC (sample4_vs_sample3)` terminated with an error exit status (1)
Command executed:
configureStrelkaSomaticWorkflow.py \
--tumor test2.paired_end.recalibrated.sorted.cram \
--normal test.paired_end.recalibrated.sorted.cram \
--referenceFasta genome.fasta \
\
\
\
--runDir strelka
sed -i s/"isEmail = isLocalSmtp()"/"isEmail = False"/g strelka/runWorkflow.py
python strelka/runWorkflow.py -m local -j 4
mv strelka/results/variants/somatic.indels.vcf.gz sample4_vs_sample3.strelka.somatic_indels.vcf.gz
mv strelka/results/variants/somatic.indels.vcf.gz.tbi sample4_vs_sample3.strelka.somatic_indels.vcf.gz.tbi
mv strelka/results/variants/somatic.snvs.vcf.gz sample4_vs_sample3.strelka.somatic_snvs.vcf.gz
mv strelka/results/variants/somatic.snvs.vcf.gz.tbi sample4_vs_sample3.strelka.somatic_snvs.vcf.gz.tbi
cat <<-END_VERSIONS > versions.yml
"NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_SOMATIC_STRELKA:STRELKA_SOMATIC":
strelka: $( configureStrelkaSomaticWorkflow.py --version )
END_VERSIONS
Command exit status:
1
Command output:
Successfully created workflow run script.
To execute the workflow, run the following script and set appropriate options:
strelka/runWorkflow.py
Command error:
[2024-12-11T09:41:31.965853Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] [2024-12-11T09:41:31.923903Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0001] [wrapperSignal] taskStderrTail 1
[2024-12-11T09:41:31.965873Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] Last 0 stderr lines from task (of 0 total lines):
[2024-12-11T09:41:31.965939Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] Failed to complete command task: 'CallGenome+callGenomeSegment_chromId_000_chr21_0003' launched from sub-workflow 'CallGenome', error code: 1, command: '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/libexec/strelka2 --region chr21:35032489-46709983 --ref genome.fasta --max-indel-size 49 --min-mapping-quality 20 --somatic-snv-rate 0.0001 --shared-site-error-rate 0.0000000005 --shared-site-error-strand-bias-fraction 0.0 --somatic-indel-rate 0.000001 --shared-indel-error-factor 2.2 --tier2-min-mapping-quality 0 --strelka-snv-max-filtered-basecall-frac 0.4 --strelka-snv-max-spanning-deletion-frac 0.75 --strelka-snv-min-qss-ref 15 --strelka-indel-max-window-filtered-basecall-frac 0.3 --strelka-indel-min-qsi-ref 40 --ssnv-contam-tolerance 0.15 --indel-contam-tolerance 0.15 --somatic-snv-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticSNVScoringModels.json --somatic-indel-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticIndelScoringModels.json --normal-align-file test.paired_end.recalibrated.sorted.cram --tumor-align-file test2.paired_end.recalibrated.sorted.cram --somatic-snv-file strelka/workspace/genomeSegment.tmpdir/somatic.snvs.unfiltered.chromId_000_chr21_0003.vcf --somatic-indel-file strelka/workspace/genomeSegment.tmpdir/somatic.indels.unfiltered.chromId_000_chr21_0003.vcf --stats-file strelka/workspace/genomeSegment.tmpdir/runStats.chromId_000_chr21_0003.xml --strelka-skip-header --strelka-chrom-depth-file strelka/workspace/chromDepth.tsv --strelka-max-depth-factor 3.0'
[2024-12-11T09:41:31.965969Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] Error Message:
[2024-12-11T09:41:31.965982Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] Anomalous task wrapper stderr output. Wrapper signal file: 'strelka/workspace/pyflow.data/logs/tmp/taskWrapperLogs/000/015/pyflowTaskWrapper.signal.txt'
[2024-12-11T09:41:31.965996Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] Logging 5 line(s) of task wrapper log output below:
[2024-12-11T09:41:31.966016Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.778274Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] wrapperStart
[2024-12-11T09:41:31.966030Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.792107Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] taskStart
[2024-12-11T09:41:31.966045Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.953327Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] taskExitCode -11
[2024-12-11T09:41:31.966059Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.954664Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] taskStderrTail 1
[2024-12-11T09:41:31.966074Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] Last 0 stderr lines from task (of 0 total lines):
[2024-12-11T09:41:32.681066Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [TaskManager] [ERROR] Failed to complete sub-workflow task: 'CallGenome' launched from master workflow, failed sub-workflow classname: 'CallWorkflow'
[2024-12-11T09:41:35.199851Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] Workflow terminated due to the following task errors:
[2024-12-11T09:41:35.199928Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] Failed to complete command task: 'CallGenome+callGenomeSegment_chromId_000_chr21_0000' launched from sub-workflow 'CallGenome', error code: 1, command: '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/libexec/strelka2 --region chr21:1-11677496 --ref genome.fasta --max-indel-size 49 --min-mapping-quality 20 --somatic-snv-rate 0.0001 --shared-site-error-rate 0.0000000005 --shared-site-error-strand-bias-fraction 0.0 --somatic-indel-rate 0.000001 --shared-indel-error-factor 2.2 --tier2-min-mapping-quality 0 --strelka-snv-max-filtered-basecall-frac 0.4 --strelka-snv-max-spanning-deletion-frac 0.75 --strelka-snv-min-qss-ref 15 --strelka-indel-max-window-filtered-basecall-frac 0.3 --strelka-indel-min-qsi-ref 40 --ssnv-contam-tolerance 0.15 --indel-contam-tolerance 0.15 --somatic-snv-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticSNVScoringModels.json --somatic-indel-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticIndelScoringModels.json --normal-align-file test.paired_end.recalibrated.sorted.cram --tumor-align-file test2.paired_end.recalibrated.sorted.cram --somatic-snv-file strelka/workspace/genomeSegment.tmpdir/somatic.snvs.unfiltered.chromId_000_chr21_0000.vcf --somatic-indel-file strelka/workspace/genomeSegment.tmpdir/somatic.indels.unfiltered.chromId_000_chr21_0000.vcf --stats-file strelka/workspace/genomeSegment.tmpdir/runStats.chromId_000_chr21_0000.xml --strelka-chrom-depth-file strelka/workspace/chromDepth.tsv --strelka-max-depth-factor 3.0'
[2024-12-11T09:41:35.199991Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] Error Message:
[2024-12-11T09:41:35.200020Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] Anomalous task wrapper stderr output. Wrapper signal file: 'strelka/workspace/pyflow.data/logs/tmp/taskWrapperLogs/000/008/pyflowTaskWrapper.signal.txt'
[2024-12-11T09:41:35.200064Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] Logging 5 line(s) of task wrapper log output below:
[2024-12-11T09:41:35.200095Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] [taskWrapper-stderr] [2024-12-11T09:41:31.770569Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0000] [wrapperSignal] wrapperStart
[2024-12-11T09:41:35.200131Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] [taskWrapper-stderr] [2024-12-11T09:41:31.782222Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0000] [wrapperSignal] taskStart
[2024-12-11T09:41:35.200163Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] [taskWrapper-stderr] [2024-12-11T09:41:31.936766Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0000] [wrapperSignal] taskExitCode -11
[2024-12-11T09:41:35.200199Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] [taskWrapper-stderr] [2024-12-11T09:41:31.938737Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0000] [wrapperSignal] taskStderrTail 1
[2024-12-11T09:41:35.200234Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0000] [taskWrapper-stderr] Last 0 stderr lines from task (of 0 total lines):
[2024-12-11T09:41:35.200264Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] Failed to complete sub-workflow task: 'CallGenome' launched from master workflow, failed sub-workflow classname: 'CallWorkflow'
[2024-12-11T09:41:35.200304Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] Failed to complete command task: 'CallGenome+callGenomeSegment_chromId_000_chr21_0001' launched from sub-workflow 'CallGenome', error code: 1, command: '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/libexec/strelka2 --region chr21:11677497-23354992 --ref genome.fasta --max-indel-size 49 --min-mapping-quality 20 --somatic-snv-rate 0.0001 --shared-site-error-rate 0.0000000005 --shared-site-error-strand-bias-fraction 0.0 --somatic-indel-rate 0.000001 --shared-indel-error-factor 2.2 --tier2-min-mapping-quality 0 --strelka-snv-max-filtered-basecall-frac 0.4 --strelka-snv-max-spanning-deletion-frac 0.75 --strelka-snv-min-qss-ref 15 --strelka-indel-max-window-filtered-basecall-frac 0.3 --strelka-indel-min-qsi-ref 40 --ssnv-contam-tolerance 0.15 --indel-contam-tolerance 0.15 --somatic-snv-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticSNVScoringModels.json --somatic-indel-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticIndelScoringModels.json --normal-align-file test.paired_end.recalibrated.sorted.cram --tumor-align-file test2.paired_end.recalibrated.sorted.cram --somatic-snv-file strelka/workspace/genomeSegment.tmpdir/somatic.snvs.unfiltered.chromId_000_chr21_0001.vcf --somatic-indel-file strelka/workspace/genomeSegment.tmpdir/somatic.indels.unfiltered.chromId_000_chr21_0001.vcf --stats-file strelka/workspace/genomeSegment.tmpdir/runStats.chromId_000_chr21_0001.xml --strelka-skip-header --strelka-chrom-depth-file strelka/workspace/chromDepth.tsv --strelka-max-depth-factor 3.0'
[2024-12-11T09:41:35.200361Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] Error Message:
[2024-12-11T09:41:35.200390Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] Anomalous task wrapper stderr output. Wrapper signal file: 'strelka/workspace/pyflow.data/logs/tmp/taskWrapperLogs/000/011/pyflowTaskWrapper.signal.txt'
[2024-12-11T09:41:35.200422Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] Logging 5 line(s) of task wrapper log output below:
[2024-12-11T09:41:35.200451Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] [2024-12-11T09:41:31.781428Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0001] [wrapperSignal] wrapperStart
[2024-12-11T09:41:35.200490Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] [2024-12-11T09:41:31.790290Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0001] [wrapperSignal] taskStart
[2024-12-11T09:41:35.200523Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] [2024-12-11T09:41:31.923019Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0001] [wrapperSignal] taskExitCode -11
[2024-12-11T09:41:35.200554Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] [2024-12-11T09:41:31.923903Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0001] [wrapperSignal] taskStderrTail 1
[2024-12-11T09:41:35.200585Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0001] [taskWrapper-stderr] Last 0 stderr lines from task (of 0 total lines):
[2024-12-11T09:41:35.200614Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] Failed to complete command task: 'CallGenome+callGenomeSegment_chromId_000_chr21_0003' launched from sub-workflow 'CallGenome', error code: 1, command: '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/libexec/strelka2 --region chr21:35032489-46709983 --ref genome.fasta --max-indel-size 49 --min-mapping-quality 20 --somatic-snv-rate 0.0001 --shared-site-error-rate 0.0000000005 --shared-site-error-strand-bias-fraction 0.0 --somatic-indel-rate 0.000001 --shared-indel-error-factor 2.2 --tier2-min-mapping-quality 0 --strelka-snv-max-filtered-basecall-frac 0.4 --strelka-snv-max-spanning-deletion-frac 0.75 --strelka-snv-min-qss-ref 15 --strelka-indel-max-window-filtered-basecall-frac 0.3 --strelka-indel-min-qsi-ref 40 --ssnv-contam-tolerance 0.15 --indel-contam-tolerance 0.15 --somatic-snv-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticSNVScoringModels.json --somatic-indel-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticIndelScoringModels.json --normal-align-file test.paired_end.recalibrated.sorted.cram --tumor-align-file test2.paired_end.recalibrated.sorted.cram --somatic-snv-file strelka/workspace/genomeSegment.tmpdir/somatic.snvs.unfiltered.chromId_000_chr21_0003.vcf --somatic-indel-file strelka/workspace/genomeSegment.tmpdir/somatic.indels.unfiltered.chromId_000_chr21_0003.vcf --stats-file strelka/workspace/genomeSegment.tmpdir/runStats.chromId_000_chr21_0003.xml --strelka-skip-header --strelka-chrom-depth-file strelka/workspace/chromDepth.tsv --strelka-max-depth-factor 3.0'
[2024-12-11T09:41:35.200672Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] Error Message:
[2024-12-11T09:41:35.200702Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] Anomalous task wrapper stderr output. Wrapper signal file: 'strelka/workspace/pyflow.data/logs/tmp/taskWrapperLogs/000/015/pyflowTaskWrapper.signal.txt'
[2024-12-11T09:41:35.200732Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] Logging 5 line(s) of task wrapper log output below:
[2024-12-11T09:41:35.200761Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.778274Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] wrapperStart
[2024-12-11T09:41:35.200792Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.792107Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] taskStart
[2024-12-11T09:41:35.200829Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.953327Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] taskExitCode -11
[2024-12-11T09:41:35.200860Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] [2024-12-11T09:41:31.954664Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0003] [wrapperSignal] taskStderrTail 1
[2024-12-11T09:41:35.200892Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0003] [taskWrapper-stderr] Last 0 stderr lines from task (of 0 total lines):
[2024-12-11T09:41:35.200921Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] Failed to complete command task: 'CallGenome+callGenomeSegment_chromId_000_chr21_0002' launched from sub-workflow 'CallGenome', error code: 1, command: '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/libexec/strelka2 --region chr21:23354993-35032488 --ref genome.fasta --max-indel-size 49 --min-mapping-quality 20 --somatic-snv-rate 0.0001 --shared-site-error-rate 0.0000000005 --shared-site-error-strand-bias-fraction 0.0 --somatic-indel-rate 0.000001 --shared-indel-error-factor 2.2 --tier2-min-mapping-quality 0 --strelka-snv-max-filtered-basecall-frac 0.4 --strelka-snv-max-spanning-deletion-frac 0.75 --strelka-snv-min-qss-ref 15 --strelka-indel-max-window-filtered-basecall-frac 0.3 --strelka-indel-min-qsi-ref 40 --ssnv-contam-tolerance 0.15 --indel-contam-tolerance 0.15 --somatic-snv-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticSNVScoringModels.json --somatic-indel-scoring-model-file /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-45ec49f1ad56587b-438a505d7b44a08705c858f9ad6e02ce/share/strelka-2.9.10-2/share/config/somaticIndelScoringModels.json --normal-align-file test.paired_end.recalibrated.sorted.cram --tumor-align-file test2.paired_end.recalibrated.sorted.cram --somatic-snv-file strelka/workspace/genomeSegment.tmpdir/somatic.snvs.unfiltered.chromId_000_chr21_0002.vcf --somatic-indel-file strelka/workspace/genomeSegment.tmpdir/somatic.indels.unfiltered.chromId_000_chr21_0002.vcf --stats-file strelka/workspace/genomeSegment.tmpdir/runStats.chromId_000_chr21_0002.xml --strelka-skip-header --strelka-chrom-depth-file strelka/workspace/chromDepth.tsv --strelka-max-depth-factor 3.0'
[2024-12-11T09:41:35.200980Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] Error Message:
[2024-12-11T09:41:35.201009Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] Anomalous task wrapper stderr output. Wrapper signal file: 'strelka/workspace/pyflow.data/logs/tmp/taskWrapperLogs/000/013/pyflowTaskWrapper.signal.txt'
[2024-12-11T09:41:35.201039Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] Logging 5 line(s) of task wrapper log output below:
[2024-12-11T09:41:35.201069Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] [taskWrapper-stderr] [2024-12-11T09:41:31.770212Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0002] [wrapperSignal] wrapperStart
[2024-12-11T09:41:35.201100Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] [taskWrapper-stderr] [2024-12-11T09:41:31.785548Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0002] [wrapperSignal] taskStart
[2024-12-11T09:41:35.201132Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] [taskWrapper-stderr] [2024-12-11T09:41:31.907218Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0002] [wrapperSignal] taskExitCode -11
[2024-12-11T09:41:35.201171Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] [taskWrapper-stderr] [2024-12-11T09:41:31.915579Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [pyflowTaskWrapper:CallGenome+callGenomeSegment_chromId_000_chr21_0002] [wrapperSignal] taskStderrTail 1
[2024-12-11T09:41:35.201202Z] [fv-az659-613.luykedtozraezimdvwvtsy4hga.cx.internal.cloudapp.net] [122769_1] [WorkflowRunner] [ERROR] [CallGenome+callGenomeSegment_chromId_000_chr21_0002] [taskWrapper-stderr] Last 0 stderr lines from task (of 0 total lines):
Work dir:
/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/83/bba38df7acae7916bdb1c96fca966c
Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`
-- Check '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/meta/nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting
-- Check '/home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/meta/nextflow.log' file for details
-[0;35m[nf-core/sarek][0;31m Pipeline completed with errors[0m-
WARN: Killing running tasks (1)
Creating env using conda: /home/runner/work/sarek/sarek/./workflows/sarek/../../modules/nf-core/multiqc/environment.yml [cache /home/runner/work/sarek/sarek/~/tests/a25df8ae6d08704eae218f7a45a79e60/work/conda/env-7da4088df3b01749-eec4e05336245ea50db6412788f8c4da]
Nextflow stderr:
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