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Added new module: msisensorpro/msitumoronly #6350
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name: msisensorpro_msitumoronly | ||||
channels: | ||||
- conda-forge | ||||
- bioconda | ||||
- defaults | ||||
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dependencies: | ||||
- bioconda::msisensor-pro=1.2.0 |
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process MSISENSORPRO_MSITUMORONLY { | ||||||
tag "$meta.id" | ||||||
label 'process_low' | ||||||
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conda "${moduleDir}/environment.yml" | ||||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||||||
'https://depot.galaxyproject.org/singularity/msisensor-pro:1.2.0--hfc31af2_0' : | ||||||
'biocontainers/msisensor-pro:1.2.0--hfc31af2_0' }" | ||||||
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input: | ||||||
tuple val(meta) , path(tumor), path(tumor_index), path(intervals) | ||||||
tuple val(meta2), path (fasta) | ||||||
tuple val(meta3), path (msisensor_baseline) | ||||||
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output: | ||||||
tuple val(meta), path("${prefix}") , emit: output_report | ||||||
tuple val(meta), path("${prefix}_dis") , emit: output_dis | ||||||
tuple val(meta), path("${prefix}_all") , emit: output_all | ||||||
tuple val(meta), path("${prefix}_unstable"), emit: output_unstable | ||||||
path "versions.yml" , emit: versions | ||||||
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when: | ||||||
task.ext.when == null || task.ext.when | ||||||
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script: | ||||||
def args = task.ext.args ?: '' | ||||||
def prefix = task.ext.prefix ?: "${meta.id}" | ||||||
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Suggested change
There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. Just because I am curious: I always see it the other way around (so that you need two times def and otherwise you run into errors). Has this changed? Is there any part of the docs you can point me to? There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. Me too, I simply copy pasted it from other modules indeed. |
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def fasta = fasta ? "-g ${fasta}" : "" | ||||||
def intervals = intervals ? "-e ${intervals} " : "" | ||||||
""" | ||||||
msisensor-pro \\ | ||||||
pro \\ | ||||||
-d ${msisensor_baseline} \\ | ||||||
-t ${tumor} \\ | ||||||
${fasta} \\ | ||||||
-o $prefix \\ | ||||||
-b ${task.cpus} \\ | ||||||
${intervals} \\ | ||||||
$args | ||||||
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cat <<-END_VERSIONS > versions.yml | ||||||
"${task.process}": | ||||||
msisensor-pro: \$(msisensor-pro 2>&1 | sed -nE 's/Version:\\sv([0-9]\\.[0-9])/\\1/ p') | ||||||
END_VERSIONS | ||||||
""" | ||||||
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stub: | ||||||
def args = task.ext.args ?: '' | ||||||
def prefix = task.ext.prefix ?: "${meta.id}" | ||||||
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""" | ||||||
mkdir ${prefix} | ||||||
mkdir ${prefix}_dis | ||||||
mkdir ${prefix}_all | ||||||
mkdir ${prefix}_unstable | ||||||
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cat <<-END_VERSIONS > versions.yml | ||||||
"${task.process}": | ||||||
msisensor-pro: \$(msisensor-pro 2>&1 | sed -nE 's/Version:\\sv([0-9]\\.[0-9])/\\1/ p') | ||||||
END_VERSIONS | ||||||
""" | ||||||
} |
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name: msisensorpro_msitumoronly | ||
description: MSIsensor-pro evaluates Microsatellite Instability (MSI) for cancer patients with next generation sequencing data. It accepts the whole genome sequencing, whole exome sequencing and target region (panel) sequencing data as input | ||
keywords: | ||
- micro-satellite-scan | ||
- msisensor-pro | ||
- msi | ||
- somatic | ||
- tumor-only | ||
tools: | ||
- msisensorpro: | ||
description: Microsatellite Instability (MSI) detection using high-throughput sequencing data. | ||
homepage: https://github.com/xjtu-omics/msisensor-pro | ||
documentation: https://github.com/xjtu-omics/msisensor-pro/wiki | ||
tool_dev_url: https://github.com/xjtu-omics/msisensor-pro | ||
doi: "10.1016/j.gpb.2020.02.001" | ||
licence: ["Custom Licence"] | ||
input: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test' ] | ||
- tumor: | ||
type: file | ||
description: BAM/CRAM/SAM file | ||
pattern: "*.{bam,cram,sam}" | ||
- tumor_index: | ||
type: file | ||
description: BAM/CRAM/SAM index file | ||
pattern: "*.{bai,crai,sai}" | ||
- intervals: | ||
type: file | ||
description: bed file containing interval information, optional | ||
pattern: "*.{bed}" | ||
- fasta: | ||
type: file | ||
description: Reference genome | ||
pattern: "*.{fasta}" | ||
- msisensor_baseline: | ||
type: file | ||
description: File containing custom list of msi regions from a panel of normals to be used as a baseline | ||
output: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test', single_end:false ] | ||
- output_report: | ||
type: file | ||
description: File containing final report with all detected microsatellites, unstable somatic microsatellites, msi score | ||
- output_dis: | ||
type: file | ||
description: File containing distribution results | ||
There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. These look like folders not files There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. They are files indeed. There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. Ok, then the stub needs to |
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- output_all: | ||
type: file | ||
description: File containing all sites | ||
- output_unstable: | ||
type: file | ||
description: File containing unstable sites | ||
- versions: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
authors: | ||
- "@FriederikeHanssen" | ||
- "@bounlu" | ||
maintainers: | ||
- "@FriederikeHanssen" |
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nextflow_process { | ||
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name "Test Process MSISENSORPRO_MSITUMORONLY" | ||
script "../main.nf" | ||
process "MSISENSORPRO_MSITUMORONLY" | ||
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tag "modules" | ||
tag "modules_nfcore" | ||
tag "msisensorpro" | ||
tag "msisensorpro/msitumoronly" | ||
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test("human - cram") { | ||
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when { | ||
process { | ||
""" | ||
input[0] = [// meta map | ||
[ id:'test'], | ||
file('https://github.com/xjtu-omics/msisensor-pro/raw/c85d51030777585ad6ab669d2f4c8e46d23f4cd8/demo/data/data4test/test_tumor_sorted.cram'), | ||
//file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), | ||
file('https://github.com/xjtu-omics/msisensor-pro/raw/c85d51030777585ad6ab669d2f4c8e46d23f4cd8/demo/data/data4test/test_tumor_sorted.cram.crai'), | ||
//file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true), | ||
[] | ||
] | ||
input[1] = [ [ id:'reference' ], // meta map | ||
file('https://raw.githubusercontent.com/xjtu-omics/msisensor-pro/c85d51030777585ad6ab669d2f4c8e46d23f4cd8/demo/data/reference/reference.fa', checkIfExists: true) | ||
] | ||
input[2] = [ [ id:'baseline' ], // meta map | ||
file('https://raw.githubusercontent.com/xjtu-omics/msisensor-pro/c85d51030777585ad6ab669d2f4c8e46d23f4cd8/data/GRCh38.baseline_TCGA.tsv', checkIfExists: true) | ||
] | ||
""" | ||
} | ||
} | ||
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then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot(process.out).match() } | ||
) | ||
} | ||
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} | ||
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test("human - cram - stub") { | ||
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options "-stub" | ||
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when { | ||
process { | ||
""" | ||
input[0] = [// meta map | ||
[ id:'test'], | ||
file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true), | ||
file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true), | ||
[] | ||
] | ||
input[1] = [ [ id:'reference' ], // meta map | ||
file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) | ||
] | ||
input[2] = [ [ id:'baseline' ], // meta map | ||
file('https://raw.githubusercontent.com/xjtu-omics/msisensor-pro/c85d51030777585ad6ab669d2f4c8e46d23f4cd8/data/GRCh38.baseline_TCGA.tsv', checkIfExists: true) | ||
] | ||
""" | ||
} | ||
} | ||
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then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot(process.out).match() } | ||
) | ||
} | ||
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} | ||
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} |
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{ | ||
"human - cram - stub": { | ||
"content": [ | ||
{ | ||
"0": [ | ||
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], | ||
"1": [ | ||
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], | ||
"2": [ | ||
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], | ||
"3": [ | ||
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], | ||
"4": [ | ||
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], | ||
"output_all": [ | ||
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], | ||
"output_dis": [ | ||
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], | ||
"output_report": [ | ||
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], | ||
"output_unstable": [ | ||
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], | ||
"versions": [ | ||
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] | ||
} | ||
], | ||
"meta": { | ||
"nf-test": "0.9.0", | ||
"nextflow": "24.04.4" | ||
}, | ||
"timestamp": "2024-09-02T11:32:08.214756" | ||
}, | ||
"human - cram": { | ||
"content": [ | ||
There was a problem hiding this comment. Choose a reason for hiding this commentThe reason will be displayed to describe this comment to others. Learn more. This looks completely empty? Probably because of the prefix issue |
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{ | ||
"0": [ | ||
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], | ||
"1": [ | ||
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], | ||
"2": [ | ||
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], | ||
"3": [ | ||
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], | ||
"4": [ | ||
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], | ||
"output_all": [ | ||
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], | ||
"output_dis": [ | ||
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], | ||
"output_report": [ | ||
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], | ||
"output_unstable": [ | ||
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], | ||
"versions": [ | ||
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] | ||
} | ||
], | ||
"meta": { | ||
"nf-test": "0.9.0", | ||
"nextflow": "24.04.4" | ||
}, | ||
"timestamp": "2024-09-02T11:31:59.733765" | ||
} | ||
} |
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msisensorpro/msitumoronly: | ||
- "modules/nf-core/msisensorpro/msitumoronly/**" |
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