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joannmudge committed Jan 10, 2025
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"\n",
"Pangenomic graphs can be very complex, often looking like \"hairballs\". Bandage allows us to zoom in on interesting regions to visualize and interact with them.\n",
"\n",
"![Compressed de Bruijn graphs “hairballs”: https://academic.oup.com/bioinformatics/article/30/24/3476/2422268](./Figures/hairballsB.png)\n",
"\n",
"\n",
"![Compressed de Bruijn graphs “hairballs”: https://academic.oup.com/bioinformatics/article/30/24/3476/2422268](./Figures/hairballsB.png)\n"
]
},
{
"cell_type": "markdown",
"metadata": {},
"source": [
"#### Graphical Fragment Assembly (GFA) format\n",
"\n",
"Bandage uses the Graphical Fragment Asembly (GFA) format.\n",
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"+ Originally developed for representing genomes during assembly\n",
"+ Now used for pangenomics\n",
"\n",
"More information on GFA format is available [here:](https://github.com/GFA-spec/GFA-spec).\n",
"\n",
"More information on GFA format is available [here:](https://github.com/GFA-spec/GFA-spec).\n"
]
},
{
"cell_type": "markdown",
"metadata": {},
"source": [
"#### Bandage\n",
"\n",
"Bandage is an interactive graph visualizer. The Bandage acronym stands for:\n",
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"\n",
"Bandage also allows you to label and color nodes by uploading comma separated value (CSV) text files with metadata.\n",
"\n",
"Details on creating CSV files can be found [here:](https://github.com/rrwick/Bandage/wiki/CSV-labels).\n",
"\n",
"Details on creating CSV files can be found [here:](https://github.com/rrwick/Bandage/wiki/CSV-labels).\n"
]
},
{
"cell_type": "markdown",
"metadata": {},
"source": [
"##### Example graph\n",
"\n",
"##### **Group exercise:**{-}\n",
"XXX in progress\n",
"\n",
"1. Copy the following example graph from inbre to your computer:\n",
"```\n",
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"4. If your have BLAST, find [CUP1](https://www.yeastgenome.org/locus/S000001095) and [YHR054C](https://www.yeastgenome.org/locus/S000001096) via BLAST\n",
" + How many copies are in the graph?\n",
" + What does the structure it’s in look like?\n",
" + Take a screenshot of the region that CUP1 is in with the gene colored\n",
"\n",
" + Take a screenshot of the region that CUP1 is in with the gene colored\n"
]
},
{
"cell_type": "markdown",
"metadata": {},
"source": [
"#### General Feature Format (GFF)\n",
"\n",
"https://genome.ucsc.edu/FAQ/FAQformat.html#format3"
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