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primer-autotools

Automatically query NCBI for qPCR primers. Basically you give it a gene symbol (e.g. GATA4) and it automatically opens Primer-BLAST on NCBI with the correct parameters set for qPCR. Parameters are customizable by changing the "param_string" in qPCR_autotool.py

Usage: python3 qPCR_autotool.py [gene symbol]

The mapping from gene symbol to RefSeq ID is provided in genemap.tsv (which was the result of a BioMart query; up to date as of September 2021). If there are multiple possible RefSeq IDs, by default the lowest one is chosen.

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Automatically make qPCR primers

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