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Krona allows hierarchical data to be explored with zooming, multi-layered pie charts. Krona charts can be created using an Excel template or KronaTools, which includes support for several bioinformatics tools and raw data formats. The interactive charts are self-contained and can be viewed with any modern web browser (see [Browser support](Browser support)).

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Publication

• Ondov BD, Bergman NH, and Phillippy AM. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30; 12(1):385.

Examples

• Miscellaneous
  • Nutrition facts for granola
  • Disk usage for a folder
• Metagenomics
  • RDP classification of 16s reads from Moving pictures of the human microbiome [Caporaso et al.]
    • Taxonomic abundance of skin microbiome samples for 4 consecutive days, with optional coloring by classification confidence
    • Comparison of taxonomic abundance in skin microbiome samples from two individuals on the same day, with optional coloring by RDP Library Comparison significance values
  • PhymmBL classification of MetaHIT human gut sample MH0072 [Qin et al.]
    • Taxonomic abundance, colored by classification confidence
  • METAREP vertical profile of South Pacific Gyre bacterioplankton [Pham et al.]
    • Taxonomic abundance, based on BLAST hits
    • Functional abundance, based on EC (Enzyme Commission) numbers of annotations
  • MG-RAST classification of acid mine drainage biofilm metagenomes [Tyson et al.]
    • Taxonomic abundance
    • Functional abundance

Tools that use Krona

• PhyloSift - phylogeny-driven metagenomic classification and comparision
• metAMOS - metagenomic assembly and classification pipeline
• MGTAXA - metagenomic classification server
• Metavir - viral metagenomic annotation server
• PhymmBL - metagenomic classification
• MG-RAST - metagenomics analysis server
• BacDive - bacterial isolation source search

Features

• Animation
  • Krona uses HTML5 to create animated transitions when zooming in and out.
• Portability
  • Krona charts are explored with web browsers, so no installation is necessary to view them.
  • Since Krona charts use HTML5, they will work without plugins using any modern browser and on any operating system.
  • Each Krona chart is contained in a single file, making them easy to share.
• Flexibility
  • Krona can create charts from XML, and charts can contain custom HTML with links to other pages. This allows straightforward integration with custom applications.
  • The use of web technologies makes it easy to include Krona charts in existing web pages.
  • Krona can render SVG snapshots for printing or publication.