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jhayer authored May 26, 2023
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Expand Up @@ -77,23 +77,6 @@ allowing comparative studies. As a last step, *baargin* performs a pangenome ana
producing the basis for the construction of a phylogenetic tree. The use of Nextflow and containers ensures
the reproducibility of the data analysis.

The Hight Throughput Sequencing technologies produce a significant amount of data,
and the DNA from multiple bacterial strains can be sequenced at the same time on a same
sequencing run. Moreover, researchers are producing genomics data all over the world on a
daily basis, notably to better understand the spread of bacterial pathogens and
their resistance to antibiotics. The analysis of this data requires the use of a
wide range of bioinformatics programs to be able to identify the genomic structure,
the genes and their functions, and among those, the genes and mutations conferring
resistance to antimicrobial drugs. In order to make the results of these analyses
comparable, it is crucial to standardize, automate and parallelize all the steps to ensure the
reproducibility of the data analysis. The workflow that we have developed allows
the user to perform a complete *in silico* analysis of a bacterial genome, from
the quality control of the raw data, to the detection of AMR genes and mutations,
on multiple datasets of bacterial strains of the same species in parallel.
It compiles and summarize the results from all the analysis steps, allowing comparative
studies, and it also performs a pangenome analysis of all the strains provided,
providing the basis for the construction of a phylogenetic tree.


# Materials and Methods

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