Releases: iqbal-lab-org/clockwork
Releases · iqbal-lab-org/clockwork
v0.12.5
v0.12.4
Add vcf/fasta filter/mask options to clockwork variant_call_one_sample
: --filter_min_dp
, --filter_min_frs
, --filter_min_gcp
, --filter_max_dp
, --fasta_allow_minos_filter_fail
, --fasta_min_frs
, --fasta_min_dp
v0.12.3
Bug fixes for ENA submissions, for where there is more than one run for a sample
v0.12.2
- Gracefully handle edge cases with
variant_call_one_sample
: cortex failing, empty reads files, missing reads files (#115) - Fixed bug in
remove_contam
when using more than one pair of input files (#116) - Fixed bug when making GVCF file, code was assuming
DP
was int instead of float (4f4df7b) - Add tag
DP_ACGT
to GVCF file (#118)
v0.12.1
Add option --no_trim
to clockwork variant_call_one_sample
to not trim reads
v0.12.0
- Update human genome to CHM13
- Variant call pipeline writes GVCF file, and fasta for MSAs that ignores indels
- fix for failing container builds by pinning nextflow to 22.10.0, which supports DSL1
v0.11.3
- update minos 0.12.5
- fix urls in decontamination download
v0.11.2
v0.11.1
Bug fix: v0.11.0 was missing the script download_tb_reference_files.pl
v0.11.0
- Use minimap2 instead of BWA. This code will work on existing reference directories made by
clockwork reference_prepare
. It will use the FASTA file in the directory. But if you rerunclockwork reference_prepare
then it will make a minimap2 index file, saving indexing on the fly. This probably makes little difference for variant calling, but could make a difference when running decontamination because that reference is huge. - Auto build singularity and docker containers
- Updates to the decontamination reference download scripts because accessions change etc.