GO:0008499 UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity with no cross reference

[raymond91125]

GO:0008499 UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity lacks EC/RHEA cross references.
One annotation reference PMID:9582303 is used by UniProt (e.g. https://www.uniprot.org/uniprotkb/Q9Y5Z6/entry) to support EC:2.4.1.86 and RHEA:53432.
N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase
an N-acetyl-beta-D-glucosaminyl derivative + UDP-alpha-D-galactose = a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl derivative + H(+) + UDP

[raymond91125]

EC:2.4.1.86 is already XREFed to GO:0047275 which is also XREFed to RHEA:16045. Rhea states that RHEA:16045 is a specific rxn to RHEA:53432.

[pgaudet]

Hi @raymond91125

Do you need help with this one?

[raymond91125]

It looks to me that EC:2.4.1.86 and RHEA:53432 should be exact match to GO:0008499 and GO:0047275 should be a child of GO:0008499. However, MetaCyc:2.4.1.86-RXN, definition XREF for GO:0047275 says something different.

[pgaudet]

These two terms should be merged (obsolete/replace).

• RHEA:53432 and RHEA:16045 have essentially the same annotations, (see screenshot below)
  
• The labels of the GO terms match the synonyms of EC: https://enzyme.expasy.org/EC/2.4.1.86

The GO label is the 'systematic name' of

Proposed actions:

• obsolete GO:0047275 glucosaminylgalactosylglucosylceramide beta-galactosyltransferase activity (0 manual annotations)
• Move EC:2.4.1.86 and RHEA:53432 to GO:0008499 as exactMatches to GO:0047275
• Change label GO:0008499 to "N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"
• Make sure the definition matches RHEA:53432

Thanks, Pascale