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Running example about HMRF #1009

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13954380607 opened this issue Aug 9, 2024 · 1 comment
Open

Running example about HMRF #1009

13954380607 opened this issue Aug 9, 2024 · 1 comment
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@13954380607
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When I run the HMRF example in Giotto, I met this question, I can't install it in my HPC, could you give me some recommendations? Thank you so much!
HMRF spatial genes
doHMRF(gobject =visium kidney,
expression values ='scaled',spatial network name ='Delaunay full',spatial genes = my spatial genes,K 5.betas =c(0,1,6),output folder = paste0(hmrf folder,'/',''Spatial genes/SG topgenes k5 scaled'))
Warning:this matrix will be converted to a dense and memory intensivebase matrix
expression matrix.txt already exists at this location, will beoverwritten
spatial genes.txt already exists at this location, will beoverwritten
spatial network.txt already exists at this location, will beoverwritten
spatial cell locations.txt already exists at this location,
will be overwritten[1] "/home/d12373/.1ocal/share/r-miniconda/envs/giotto env/bin/pvthon /home/d12373/,conda/enys/r eny/lib/R/library/Giotto/python/reader2.py -1 "/home/d12373/data/results/HMRF_results//spatial_genes/sG topgenes_k5 _scaled/spatial cell locations.txt" -g"/home/d12373/data/results/HMRF _results//spatial genes/sG topgenes _k5scaled/spatial_genes.txt" -n "/home/d12373/data/results/HMRF_results//spatial_genes/sG topgenes_k5_scaled/spatial network.txt" -e "/home/d12373/data/results/HMRF results//spatial genes/sG topgenes k5 scaled/exprssion matrix.txt" -0 "/home/d12373/data/results/HMRF results//spatial genes/sG topgenes k5 scaled/result..patial.zscore"-atest-k5-b016-t1e-10 -z none -s 100 -i 100"sh:iava:command not found
last):Traceback(mostrecent cal1File "/home/d12373/.conda/envs/renv/lib/R/library/Giotto/python/reader2.py",line 122,in if java exists:raise(KeyError(java error_message))KevError: 'Error: iava not found. This function uses a java functionality. Please ensure iava is installedhttps://www.java.com/en/download/

@13954380607 13954380607 added the bug Something isn't working label Aug 9, 2024
@josschavezf
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@13954380607, you can try using the Giotto singularity image. Here is a tutorial: https://drieslab.github.io/Giotto_website/articles/singularity.html

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