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Original file line number | Diff line number | Diff line change |
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from brainglobe_space import AnatomicalSpace | ||
from napari.layers import Image, Labels, Points | ||
from napari.utils.notifications import show_info | ||
from napari.viewer import Viewer | ||
from qtpy.QtWidgets import ( | ||
QComboBox, | ||
QFormLayout, | ||
QGroupBox, | ||
QHBoxLayout, | ||
QPushButton, | ||
QWidget, | ||
) | ||
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||
class Reorient(QWidget): | ||
"""Widget to reorient images to a standard anatomical space.""" | ||
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def __init__(self, napari_viewer: Viewer, parent=None): | ||
super().__init__(parent=parent) | ||
self.viewer = napari_viewer | ||
self.setLayout(QFormLayout()) | ||
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self._create_reorient_group() | ||
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def _create_reorient_group(self): | ||
self.reorient_groupbox = QGroupBox("Reorient to standard space") | ||
self.reorient_groupbox.setLayout(QFormLayout()) | ||
self.layout().addRow(self.reorient_groupbox) | ||
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avail_labels = AnatomicalSpace.lims_labels | ||
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# Enter labels for source space | ||
source_origin_row = QHBoxLayout() | ||
self.source_origin1 = QComboBox(parent=self.reorient_groupbox) | ||
self.source_origin1.addItems(avail_labels) | ||
self.source_origin1.setCurrentIndex(0) | ||
source_origin_row.addWidget(self.source_origin1) | ||
self.source_origin2 = QComboBox(parent=self.reorient_groupbox) | ||
self.source_origin2.addItems(avail_labels) | ||
self.source_origin2.setCurrentIndex(2) | ||
source_origin_row.addWidget(self.source_origin2) | ||
self.source_origin3 = QComboBox(parent=self.reorient_groupbox) | ||
self.source_origin3.addItems(avail_labels) | ||
self.source_origin3.setCurrentIndex(5) | ||
source_origin_row.addWidget(self.source_origin3) | ||
self.reorient_groupbox.layout().addRow( | ||
"Source origin:", source_origin_row | ||
) | ||
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# Enter labels for target space | ||
target_origin_row = QHBoxLayout() | ||
self.target_origin1 = QComboBox(parent=self.reorient_groupbox) | ||
self.target_origin1.addItems(avail_labels) | ||
self.target_origin1.setCurrentIndex(1) | ||
target_origin_row.addWidget(self.target_origin1) | ||
self.target_origin2 = QComboBox(parent=self.reorient_groupbox) | ||
self.target_origin2.addItems(avail_labels) | ||
self.target_origin2.setCurrentIndex(2) | ||
target_origin_row.addWidget(self.target_origin2) | ||
self.target_origin3 = QComboBox(parent=self.reorient_groupbox) | ||
self.target_origin3.addItems(avail_labels) | ||
self.target_origin3.setCurrentIndex(5) | ||
target_origin_row.addWidget(self.target_origin3) | ||
self.reorient_groupbox.layout().addRow( | ||
"Target origin:", target_origin_row | ||
) | ||
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self.reorient_button = QPushButton( | ||
"Reorient selected layers", parent=self.reorient_groupbox | ||
) | ||
self.reorient_button.clicked.connect(self.reorient_layers) | ||
self.reorient_groupbox.layout().addRow(self.reorient_button) | ||
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def reorient_layers(self): | ||
"""Reorient selected layers to the target space.""" | ||
selected_layers = self.viewer.layers.selection | ||
selected_stacks = [ | ||
layer | ||
for layer in selected_layers | ||
if isinstance(layer, Image) or isinstance(layer, Labels) | ||
] | ||
selected_points = [ | ||
layer for layer in selected_layers if isinstance(layer, Points) | ||
] | ||
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if not selected_layers: | ||
show_info("No layers selected. Select layers to reorient.") | ||
return | ||
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source_origin = [ | ||
self.source_origin1.currentText(), | ||
self.source_origin2.currentText(), | ||
self.source_origin3.currentText(), | ||
] | ||
target_origin = [ | ||
self.target_origin1.currentText(), | ||
self.target_origin2.currentText(), | ||
self.target_origin3.currentText(), | ||
] | ||
source_origin = "".join(source_origin) | ||
target_origin = "".join(target_origin) | ||
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for layer in selected_points: | ||
if not selected_stacks: | ||
show_info( | ||
"Cannot reorient Points layer alone. Please select " | ||
"at least one Image or Labels layer along with the " | ||
"Points layer." | ||
) | ||
return | ||
source_stack_shape = selected_stacks[0].data.shape | ||
source_space = AnatomicalSpace( | ||
source_origin, shape=source_stack_shape | ||
) | ||
layer.data = source_space.map_points_to(target_origin, layer.data) | ||
layer.name = f"{layer.name}_space-{target_origin}" | ||
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for layer in selected_stacks: | ||
interp_order = 0 if isinstance(layer, Labels) else 3 | ||
source_space = AnatomicalSpace( | ||
source_origin, shape=layer.data.shape | ||
) | ||
layer.data = source_space.map_stack_to( | ||
target_origin, layer.data, interp_order=interp_order | ||
) | ||
layer.name = f"{layer.name}_orig-{target_origin}" | ||
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show_info("Layers reoriented to target space.") |
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