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I am using STAR/2.7.11b for the alignment step. I am using the following command
STAR --runMode alignReads --runThreadN 16 --genomeDir $star_index --outSAMattributes All --outFilterType BySJout --outFilterMultimapNmax 10 --outFilterMultimapScoreRange 1 --outFilterScoreMin 10 --outFilterMismatchNmax 5 --alignEndsType Local --outSAMtype BAM SortedByCoordinate --readFilesCommand zcat --outTmpDir $tempDIR --readFilesIn $R1 $R2 --quantMode TranscriptomeSAM GeneCounts --outFileNamePrefix sample1 \n
I am able to index the genome-based bam file but not the transcriptome bam file. What is the best way to index the transcriptome bam file? Thanks!
The text was updated successfully, but these errors were encountered:
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I am using STAR/2.7.11b for the alignment step. I am using the following command
STAR --runMode alignReads --runThreadN 16 --genomeDir $star_index --outSAMattributes All --outFilterType BySJout --outFilterMultimapNmax 10 --outFilterMultimapScoreRange 1 --outFilterScoreMin 10 --outFilterMismatchNmax 5 --alignEndsType Local --outSAMtype BAM SortedByCoordinate --readFilesCommand zcat --outTmpDir $tempDIR --readFilesIn $R1 $R2 --quantMode TranscriptomeSAM GeneCounts --outFileNamePrefix sample1 \n
I am able to index the genome-based bam file but not the transcriptome bam file. What is the best way to index the transcriptome bam file? Thanks!
The text was updated successfully, but these errors were encountered: