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Issue with tracer test #60

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Edenapple opened this issue Feb 12, 2018 · 1 comment
Open

Issue with tracer test #60

Edenapple opened this issue Feb 12, 2018 · 1 comment

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@Edenapple
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The input command is:
tracer test -p 3 -c /home/temp/binaries/tracer-master/tracer.conf -o tracer_test

The error message is (in Trinity):
CMD: /home/temp/binaries/trinityrnaseq-Trinity-v2.5.1/util/support_scripts/get_Trinity_gene_to_trans_map.pl /home/temp/disk1/@home/edenapple/Desktop/Practice/Mouse/tracer/tracer_test/results/cell1/Trinity_output/Trinity_cell1_TCR_B.Trinity.fasta > /home/temp/disk1/@home/edenapple/Desktop/Practice/Mouse/tracer/tracer_test/results/cell1/Trinity_output/Trinity_cell1_TCR_B.Trinity.fasta.gene_trans_map
shell-init: error retrieving current directory: getcwd: cannot access parent directories: No such file or directory

The error message is (in IGBlast):
Traceback (most recent call last):
File "/home/temp/anaconda2/bin/tracer", line 11, in
load_entry_point('tracer==0.5', 'console_scripts', 'tracer')()
File "/home/temp/anaconda2/lib/python2.7/site-packages/tracer-0.5-py2.7.egg/tracerlib/launcher.py", line 43, in launch
Task().run()
File "/home/temp/anaconda2/lib/python2.7/site-packages/tracer-0.5-py2.7.egg/tracerlib/tasks.py", line 1201, in run
loci=['A', 'B'], max_junc_len=50).run()
File "/home/temp/anaconda2/lib/python2.7/site-packages/tracer-0.5-py2.7.egg/tracerlib/tasks.py", line 372, in run
self.quantify(cell)
File "/home/temp/anaconda2/lib/python2.7/site-packages/tracer-0.5-py2.7.egg/tracerlib/tasks.py", line 638, in quantify
tpm = counts[receptor][locus][rec.contig_name]
KeyError: 'TRINITY_DN1_c0_g1_i3'

@mstubb
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mstubb commented Feb 12, 2018

Hi,

Please have a look at Issue #55 and see if any of the suggestions there help here. If not, please let me know.

Best,

Mike

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