###WisePair
###License See LICENSE.md
####What is it used for? WisePair is a set of python scripts built to address two issues in individual-based genetic tracking studies.
- Sampling scheme design to maximize resampling a set number of individuals.
- Confidently identifying individuals who have been resampled in a data-set.
####There are 3 main scripts in the core of WisePair:
beanbag.py
The beanbag.py script is designed to build virtual individual genotypes of a population to be used in simulated sampling. This design is based on user-supplied criteria such as number of individuals in the population, number of loci, and allelic frequencies. In addition, this script incorporates genotyping error rates during sampling.
wisepair.py
The second script, wisepair.py, determines the number of re-samples within a specified data set (real or virtual) through allelic pairwise comparisons. wisepair.py determines the number of re-samples within a virtual data set, determines the number of re-samples within an actual data using specified threshold simulations, estimates the number of errors for re-samples, and determines whether re-samples can be distinguished from non-re-samples.
optimagic.py
The final script, optimagic.py, utilizes outputs from both beanbag.py and wisepair.py to develop optimal sampling designs for individual based studies. beanbag.py and wisepair.py are used to produce a threshold “score” with which we could compare samples to the field data set and subsequent simulations in optimagic.py
####Usage:
####beanbag.py
To build a virtual population, beanbag.py requires 2 input files and several flags.
INFILE is a JSON format file which contains loci names, alleles for each loci and allelic frequencies for each allele. ERRFILE is a JSON format file which contains loci names, allelic dropout rates, and false allele rates. Currently there is no function to build these files, so they must be built manually.
Examples of both files can be found in the sample_data directory.
The required flags are: [-t], [-i], [-e], [-b], [-s], [-p], [-o]
Optional flags are: [-l], [-r]
An example command is found in the sample_data directory, in the example_cmds.txt file.
usage: beanbag.py [-h] -t SIMTYPE -i INFILE -e ERRFILE -b BOUTLIMIT -s
SAMPLELIMIT [-l PERBOUT] -p POPSIZE [-r PERCENTPRESENT] -o
OUTFILE
optional arguments:
-h, --help show this help message and exit
-t SIMTYPE, --simtype SIMTYPE
Specify which type of simulation type; currently only
simfile
-i INFILE, --infile INFILE
Specify path to input frequency file; SIMFILE
-e ERRFILE, --errfile ERRFILE
Specify path to input error file; JSON format
-b BOUTLIMIT, --boutlimit BOUTLIMIT
Total number of sampling bouts for a season.
-s SAMPLELIMIT, --samplelimit SAMPLELIMIT
Total number of samples for a season.
-l PERBOUT, --perbout PERBOUT
Number of samples for each bout. [default = False]
-p POPSIZE, --popsize POPSIZE
Specify the size of a virtual population.
-r PERCENTPRESENT, --percentpresent PERCENTPRESENT
Population present at each sampling between 0-1
[default = 1]
-o OUTFILE, --outfile OUTFILE
Specify the output file.
####wisepair.py
wisepair.py takes 2 inputs, the directory to the beanbag.py output and a list of the output files.
If real data is being analyzed aswell, those files should be run separatly (and after) from the simulated data so that the model_stats file is already created.
usage: wisepair.py [-h] [-s SIMDIR] [-l SIMLIST]
optional arguments:
-h, --help show this help message and exit
-s SIMDIR, --simdir SIMDIR
path to simulation file directory.
-l SIMLIST, --simlist SIMLIST
list of simulation files.
####optimagic.py
This script combines requirements of beanbag.py and wisepair.py along with some ranges that allow for multiple schemes to be tested.
Real data can be analyzed after models are built for comparison.
usage: optimagic.py [-h] [-s SIMDIR] -p POPSIZE [-x PERPOP] -l SAMPRAN -b
BOUTRAN -r RESAMPMIN -n NUMMINRAN [-u RUNSIM] [-m ITERLIM]
[-t SIMTYPE] [-i SIMFILE] [-e ERRFILE]
optional arguments:
-h, --help show this help message and exit
-s SIMDIR, --simdir SIMDIR
path to simulation file directory.
-p POPSIZE, --popsize POPSIZE
population size.
-x PERPOP, --perpop PERPOP
population size present at site.
-l SAMPRAN, --sampran SAMPRAN
range of samples per bout.
-b BOUTRAN, --boutran BOUTRAN
range of bouts.
-r RESAMPMIN, --resampmin RESAMPMIN
minimum individuals resampled over season.
-n NUMMINRAN, --numminran NUMMINRAN
range for minimum number of resamples per resampled
individual.
-u RUNSIM, --runsim RUNSIM
use simerator to run simulations.
-m ITERLIM, --iterlim ITERLIM
number of iterations during simulation mode.
-t SIMTYPE, --simtype SIMTYPE
specitfy type of input simulation format: virtpop,
simfile, genepop.
-i SIMFILE, --simfile SIMFILE
path to simulation input file.
-e ERRFILE, --errfile ERRFILE
Specify path to input error file; JSON format
###Running the simulations to find sampling scheme
####Example command to run beanbag.py
This example builds a virtual population of 300 individuals. It then precedes to sample this population 5 times, totaling 125 samples (25 samples/bout). The -r flag indicates that 50% of the population is present at the start of each bout.
python beanbag.py -t simfile -i real_data_frequencies.json -e pedant_error_rates.json -b 5 -s 125 -p 300 -r 0.5 -o example_output.csv####Example command to run wisepair.py
This command takes the output from beanbag.py and runs it through wisepair. The output is a scoring matrix for the sample comparisons, a report visualization, and a stats file of the simulations.
python wisepair.py -s [location of beanbag.py output csv] -l [csv filename with no path]####Example command to run optimagic.py
This command runs a optimization run to determine which sampling scheme would work best if 2 individuals need to be resampled at least 3 times from a population of 100 individuals.
The outputs from beanbag.py and wisepair.py are sent to the -s path in a directory called OM_simdir, and 2 csv files are produced which separate good and bad sampling schemes.
python optimagic.py -s [path to output] -p 100 -x 0.5 -l 20,25 -b 5,8 -r 2 -n 3 -u True -m 1 -t simfile -i real_data_frequencies.json -e pedant_error_rates.json###Analyzing real data with simulations
####Example command to run optimagic.py
Run optimagic.py with the bounds of the real data sampling scheme at a higher iteration limit to build a model for the real data.
python optimagic.py -s [path to output] -p 100 -x 0.5 -l 30 -b 5 -r 2 -n 3 -u True -m 10 -t simfile -i real_data_frequencies.json -e pedant_error_rates.json####Example command to run wisepair.py
Copy the model_stats.tsv from the optimagic output directory to the same location as the real data files.
Output from this will be identified resampled individuals.
python wisepair.py -s [location of beanbag.py output csv] -l [csv filename with no path]