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## Perform group FDR estimation for different identification groups | ||
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##### [FragPipe](https://fragpipe.nesvilab.org) can be downloaded [here](https://github.com/Nesvilab/FragPipe/releases). Follow the instructions on that same Releases page to launch the program. See [here](https://fragpipe.nesvilab.org/docs/tutorial_fragpipe.html#configure-fragpipe) for help configuring FragPipe. | ||
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FragPipe can perform a group FDR estimation to calculate FDRs for each group separately. Then groups can be the number of enzymatic termini (_i.e._ 0, 1, 2) and the protein evidence levels encoded in the FASTA file. | ||
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The group FDR approach supports most workflows. After loading a specific workflow, go to the MSFragger tab to select the group type. | ||
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![](https://raw.githubusercontent.com/Nesvilab/FragPipe/gh-pages/images/group_fdr_1.png) | ||
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Then, go to the `Validation` tab, and add `--group` to the `FDR Filter and Report` command box | ||
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![](https://raw.githubusercontent.com/Nesvilab/FragPipe/gh-pages/images/group_fdr_2.png) | ||
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The rest of the part is the same as that in a normal FragPipe analysis. | ||
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To make FragPipe recognize the encoded protein evidence, the protein header in the FASTA file must have the `PE=` keyword. The numbers after `PE=` identify different groups. [Here](https://github.com/bassanilab/CircRNA_MS_ref_fasta) has a handy script to revise the protein headers. | ||
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<br> | ||
<br> | ||
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#### Key References | ||
_coming soon_ | ||
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<br> | ||
<br> | ||
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#### [Back to FragPipe homepage](https://fragpipe.nesvilab.org/) |