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Add the group FDR tutorial.
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fcyu committed Dec 17, 2023
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## Perform group FDR estimation for different identification groups

##### [FragPipe](https://fragpipe.nesvilab.org) can be downloaded [here](https://github.com/Nesvilab/FragPipe/releases). Follow the instructions on that same Releases page to launch the program. See [here](https://fragpipe.nesvilab.org/docs/tutorial_fragpipe.html#configure-fragpipe) for help configuring FragPipe.

FragPipe can perform a group FDR estimation to calculate FDRs for each group separately. Then groups can be the number of enzymatic termini (_i.e._ 0, 1, 2) and the protein evidence levels encoded in the FASTA file.

The group FDR approach supports most workflows. After loading a specific workflow, go to the MSFragger tab to select the group type.

![](https://raw.githubusercontent.com/Nesvilab/FragPipe/gh-pages/images/group_fdr_1.png)

Then, go to the `Validation` tab, and add `--group` to the `FDR Filter and Report` command box

![](https://raw.githubusercontent.com/Nesvilab/FragPipe/gh-pages/images/group_fdr_2.png)

The rest of the part is the same as that in a normal FragPipe analysis.

To make FragPipe recognize the encoded protein evidence, the protein header in the FASTA file must have the `PE=` keyword. The numbers after `PE=` identify different groups. [Here](https://github.com/bassanilab/CircRNA_MS_ref_fasta) has a handy script to revise the protein headers.



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#### Key References
_coming soon_



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#### [Back to FragPipe homepage](https://fragpipe.nesvilab.org/)

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