Script to convert Roary to Fripan.

bin/roary2fripan.pl

@@ -0,0 +1,150 @@
+#!/usr/bin/env perl
+use warnings;
+use strict;
+use Data::Dumper;
+use List::Util qw(min max sum);
+use List::MoreUtils qw(uniq all any);
+use Text::CSV;
+
+my $PARALOG_SEP = ',';
+my $TAXA_COL = 14;
+my $MISSING = '*';
+
+my(@Options, $verbose, $roary, $prefix);
+setOptions();
+$prefix or err("Please provide a --prefix for the fripan output files");
+
+open my $ROARY, '<', $roary or err("Could not open --roary file: $roary");
+msg("Opened: $roary");
+
+my %fh;
+for my $ext ('proteinortho', 'strains', 'descriptions') {
+  my $fname = "$prefix.$ext";
+  open $fh{$ext}, '>', $fname or err("Could not open '$fname' for writing.");
+  msg("Will save to: $fname");
+}
+
+# read gene_presence_absence.csv from stdin
+#  0  "Gene"
+#  1  "Non-unique Gene name"
+#  2  "Annotation"
+#  3  "No. isolates"
+#  4  "No. sequences"
+#  5  "Avg sequences per isolate"
+#  6  "Genome Fragment"
+#  7  "Order within Fragment"
+#  8  "Accessory Fragment"
+#  9  "Accessory Order with Fragment"
+# 10  "QC"
+# 11  "Min group size nuc"
+# 12  "Max group size nuc"
+# 13  "Avg group size nuc"
+
+# XXX.proteinortho
+#0  # Species
+#1  Genes
+#2  Alg.-Conn.
+#3  BPH0693
+#4  BPH0694
+#5  BPH0695
+#6  BPH0696
+# line 2
+#0  3
+#1  3
+#2  1
+#3  BPH0693_00454
+#4  *
+#5  BPH0695_01451
+#6  BPH0696_02515
+
+my $csv = Text::CSV->new() or die $!;
+my $count=0;
+my $N=0;
+my @id;
+my $G=0;
+
+while (my $row = $csv->getline($ROARY) ) {
+  if ($count == 0) {
+    @id = splice @$row, $TAXA_COL;
+    $N = scalar(@id);
+    msg("Found $N taxa: $id[0] ... $id[-1]");
+    print {$fh{proteinortho}} tsv('# Species', 'Genes', 'Alg.-Conn.', @id);
+    print {$fh{strains}} tsv('ID', 'Order');
+    print {$fh{strains}} tsv($id[$_], $_+1) for (0 .. $#id);
+  }
+  else {
+    my @cds = map { $_ ? $_ : $MISSING } @$row[$TAXA_COL .. $#$row];
+    map { s/\t/,/g } @cds;
+    print {$fh{proteinortho}} tsv( @$row[3,4,5], @cds );
+    for my $lt (grep { $_ ne $MISSING } @cds) {
+      my $desc = $row->[2] || 'unannotated protein';
+      $desc .= ' ('.$row->[0].')' if $row->[0];
+      print {$fh{descriptions}} tsv($lt, $desc);
+      $G++;
+    }
+  }
+  $count++;
+}
+
+$count--;
+msg("Processed $count ortholog clusters and $G genes.");
+msg("Done.");
+
+
+#----------------------------------------------------------------------
+sub tsv {
+  if (any { m/\t/ } @_) { 
+    err("Found <TAB> within a cell row: @_")
+  }
+  return join("\t", @_)."\n";
+}
+
+#----------------------------------------------------------------------
+
+sub msg {
+  print STDERR "@_\n";
+}
+
+#----------------------------------------------------------------------
+
+sub err {
+  print STDERR "ERROR: @_\n";
+  exit(1);
+}
+
+#----------------------------------------------------------------------
+# Option setting routines
+
+sub setOptions {
+  use Getopt::Long;
+
+  @Options = (
+    {OPT=>"help",    VAR=>\&usage,             DESC=>"This help"},
+    {OPT=>"verbose!",  VAR=>\$verbose, DEFAULT=>0, DESC=>"Verbose output"},
+    {OPT=>"roary=s",   VAR=>\$roary,  DEFAULT=>'gene_presence_absence.csv', DESC=>"Roary ortholog matrix file"},
+    {OPT=>"prefix=s",  VAR=>\$prefix,  DEFAULT=>'', DESC=>"Prefix for Fripan output files"},
+  );
+
+#  (!@ARGV) && (usage());
+
+  &GetOptions(map {$_->{OPT}, $_->{VAR}} @Options) || usage();
+
+  # Now setup default values.
+  foreach (@Options) {
+    if (defined($_->{DEFAULT}) && !defined(${$_->{VAR}})) {
+      ${$_->{VAR}} = $_->{DEFAULT};
+    }
+  }
+}
+
+sub usage {
+  print "Usage: $0 [options] gene_presence_absence.csv\n";
+  foreach (@Options) {
+    printf "  --%-13s %s%s.\n",$_->{OPT},$_->{DESC},
+           defined($_->{DEFAULT}) ? " (default '$_->{DEFAULT}')" : "";
+  }
+  exit(1);
+}
+
+#----------------------------------------------------------------------
+