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* cleanup of documentation * docs: added name * fixed linting error * with local linter * Update about.md * fit * fmt * fmt * pin python for ragoo * rev --------- Co-authored-by: Alexander Thomas <[email protected]>
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# About | ||
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## The team | ||
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- Alexander Thomas | ||
- Thomas Battenfeld | ||
- Simon Magin | ||
- Johannes Koester | ||
- Folker Meyer | ||
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## Tools used in UnCoVar | ||
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This project wouldn't be possible without several open source tools and libraries: | ||
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| Tool | Link | | ||
| -------------- | --------------------------------------------------- | | ||
| Altair | <www.doi.org/10.21105/joss.01057> | | ||
| BAMClipper | <www.doi.org/10.1038/s41598-017-01703-6> | | ||
| BCFtools | <www.doi.org/10.1093/gigascience/giab008> | | ||
| BEDTools | <www.doi.org/10.1093/bioinformatics/btq033> | | ||
| Biopython | <www.doi.org/10.1093/bioinformatics/btp163> | | ||
| bwa | <www.doi.org/10.1093/bioinformatics/btp324> | | ||
| Covariants | <www.github.com/hodcroftlab/covariants> | | ||
| delly | <www.doi.org/10.1093/bioinformatics/bts378> | | ||
| ensembl-vep | <www.doi.org/10.1186/s13059-016-0974-4> | | ||
| entrez-direct | <www.ncbi.nlm.nih.gov/books/NBK179288> | | ||
| fastp | <www.doi.org/10.1093/bioinformatics/bty560> | | ||
| FastQC | <www.bioinformatics.babraham.ac.uk/projects/fastqc> | | ||
| fgbio | <www.github.com/fulcrum-genomics/fgbio> | | ||
| FreeBayes | <www.arxiv.org/abs/1207.3907> | | ||
| intervaltree | <www.github.com/chaimleib/intervaltree> | | ||
| Jupyter | <www.jupyter.org> | | ||
| kallisto | <www.doi.org/10.1038/nbt.3519> | | ||
| Kraken2 | <www.doi.org/10.1186/s13059-019-1891-0> | | ||
| Krona | <www.doi.org/10.1186/1471-2105-12-385> | | ||
| mason | <publications.imp.fu-berlin.de/962> | | ||
| MEGAHIT | <www.doi.org/10.1093/bioinformatics/btv033> | | ||
| Minimap2 | <www.doi.org/10.1093/bioinformatics/bty191> | | ||
| MultiQC | <www.doi.org/10.1093/bioinformatics/btw354> | | ||
| pandas | <pandas.pydata.org> | | ||
| Picard | <broadinstitute.github.io/picard> | | ||
| PySAM | <www.doi.org/10.11578/dc.20190903.1> | | ||
| QUAST | <www.doi.org/10.1093/bioinformatics/btt086> | | ||
| RaGOO | <www.doi.org/10.1186/s13059-019-1829-6> | | ||
| ruamel.yaml | <www.sourceforge.net/projects/ruamel-yaml> | | ||
| Rust-Bio-Tools | <www.github.com/rust-bio/rust-bio-tools> | | ||
| SAMtools | <www.doi.org/10.1093/bioinformatics/btp352> | | ||
| Snakemake | <www.doi.org/10.12688/f1000research.29032.1> | | ||
| sourmash | <www.doi.org/10.21105/joss.00027> | | ||
| SPAdes | <www.doi.org/10.1089/cmb.2012.0021> | | ||
| SVN | <www.doi.org/10.1142/s0219720005001028> | | ||
| Tabix | <www.doi.org/10.1093/bioinformatics/btq671> | | ||
| Trinity | <www.doi.org/10.1038/nprot.2013.084> | | ||
| Varlociraptor | <www.doi.org/10.1186/s13059-020-01993-6> | | ||
| Vega-Lite | <www.doi.org/10.1109/TVCG.2016.2599030> | | ||
| Velvet | <www.doi.org/10.1101/gr.074492.107> | | ||
| vembrane | <www.github.com/vembrane/vembrane> | |
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# FAQ | ||
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## Where to find the source code? | ||
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[https://github.com/IKIM-Essen/uncovar](https://github.com/IKIM-Essen/uncovar) | ||
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## How to execute the workflow? | ||
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Once you have | ||
[installed mamba](https://mamba.readthedocs.io/en/latest/installation/micromamba-installation.html), | ||
just follow the instructions | ||
[here](https://snakemake.github.io/snakemake-workflow-catalog?usage=IKIM-Essen/uncovar). |
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# Welcome to the Documentation of UnCoVar | ||
# UnCoVar -- an open, extensible framework for virus genome analysis | ||
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<picture> | ||
<source media="(prefers-color-scheme: dark)" srcset="https://github.com/IKIM-Essen/uncovar/assets/77535027/8e17c6fc-ff7a-4c25-afc9-7888036d693e"> | ||
<source media="(prefers-color-scheme: light)" srcset="https://github.com/IKIM-Essen/uncovar/assets/77535027/c99f5a94-749b-422e-b319-1e3700d40a8e"> | ||
<img alt="UnCoVar Logo dark/light"> | ||
</picture> | ||
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[![Snakemake](https://img.shields.io/badge/snakemake-≥6.3.0-brightgreen.svg)](https://snakemake.bitbucket.io) | ||
[![GitHub actions status](https://github.com/koesterlab/snakemake-workflow-sars-cov2/workflows/Tests/badge.svg?branch=master)](https://github.com/koesterlab/snakemake-workflow-sars-cov2/actions?query=branch%3Amaster+workflow%3ATests) | ||
[![Docker Repository on Quay](https://quay.io/repository/uncovar/uncovar/status "Docker Repository on Quay")](https://quay.io/repository/uncovar/uncovar) | ||
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UnCoVar is a reproducible and scalable workflow for transparent and robust | ||
SARS-CoV-2 variant calling and lineage assignment with comprehensive reporting. | ||
A Reproducible and Scalable Workflow for Transparent and Robust Virus Variant Calling and Lineage Assignment using SARS-CoV-2 as an example. | ||
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- Using state of the art tools, easily extended for other viruses | ||
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![UnCoVar tools](./assets/tools.png) | ||
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- Tools and database updates for critical components via Conda | ||
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- Built using modern design patterns with Conda and SnakeMake | ||
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- Extensible and easy to customize | ||
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<div class="row"> | ||
<div class="column"> | ||
<div class="card"> | ||
<div class="card-title"> | ||
<h3>Latest SARS-CoV-2 Lineage Assignment</h3> | ||
</div> | ||
<p> | ||
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Proin molestie | ||
neque scelerisque, bibendum nulla eget. | ||
</p> | ||
</div> | ||
</div> | ||
<div class="column"> | ||
<div class="card"> | ||
<div class="card-title"> | ||
<h3>Comprehensive Reporting</h3> | ||
</div> | ||
<p> | ||
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Proin molestie | ||
neque scelerisque, bibendum nulla eget. | ||
</p> | ||
</div> | ||
</div> | ||
</div> | ||
- Customizable reporting with comprehensive visualization | ||
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<div class="row"> | ||
<div class="column"> | ||
<div class="card"> | ||
<div class="card-title"> | ||
<h3>Submission Ready Genomes</h3> | ||
</div> | ||
<p> | ||
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Proin molestie | ||
neque scelerisque, bibendum nulla eget. | ||
</p> | ||
</div> | ||
</div> | ||
<div class="column"> | ||
<div class="card"> | ||
<div class="card-title"> | ||
<h3>Easy to Customize</h3> | ||
</div> | ||
<p> | ||
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Proin molestie | ||
neque scelerisque, bibendum nulla eget. | ||
</p> | ||
</div> | ||
</div> | ||
</div> | ||
![UnCoVar visuals](./assets/uncovar-displays.png) | ||
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[Getting Started](getting-started/){ .md-button } | ||
[User Guide](user-guide/){ .md-button } | ||
- Submission Ready Genomes | ||
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[introductory tutorial]: getting-started.md | ||
[User Guide]: user-guide/index.md |
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# Installation | ||
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## Installing | ||
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## Maintenance | ||
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