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I am trying to use genomation and methylKit to analyze some RRBS data.
I identified the identified the significantly differentially methylated cytosines (q-value threshold of 0.01 and a minimum change in percent methylation of 25%) and used for the gene annotation analysis a .gtf file.
I ended up using two scripts (gtfToGenePred and genePredToBed) from http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/ to convert my .gtf file to .bed
Everything seems to have worked fine until I tried to use "getTargetAnnotationStats" which gives me a error message (please see below):
#R code
Hi,
I am trying to use genomation and methylKit to analyze some RRBS data.
I identified the identified the significantly differentially methylated cytosines (q-value threshold of 0.01 and a minimum change in percent methylation of 25%) and used for the gene annotation analysis a .gtf file.
I ended up using two scripts (gtfToGenePred and genePredToBed) from http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/ to convert my .gtf file to .bed
Everything seems to have worked fine until I tried to use "getTargetAnnotationStats" which gives me a error message (please see below):
#R code
percentage of target features overlapping with annotation:
promoter exon intron intergenic
3.93 6.74 35.39 57.87
percentage of target features overlapping with annotation:
(with promoter > exon > intron precedence):
promoter exon intron intergenic
3.93 4.49 33.71 57.87
percentage of annotation boundaries with feature overlap:
promoter exon intron
0.03 0.00 0.01
summary of distances to the nearest TSS:
Min. 1st Qu. Median Mean 3rd Qu. Max.
132 4327 13174 233837 53026 5049539
Could you help me to figure out what is the issue?
Any help on this is much appreciated.
Cheers
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