diff --git a/tests/drivers/uart/uart_async_api/boards/atsamd21_xpro.overlay b/tests/drivers/uart/uart_async_api/boards/atsamd21_xpro.overlay
new file mode 100644
index 00000000000000..dd7bf28f3029d8
--- /dev/null
+++ b/tests/drivers/uart/uart_async_api/boards/atsamd21_xpro.overlay
@@ -0,0 +1,11 @@
+/* SPDX-License-Identifier: Apache-2.0 */
+
+&sercom1 {
+	/* Internally loop-back TX and RX on PAD0 */
+	rxpo = <0>;
+	txpo = <0>;
+
+	/* Configure DMA channels for async operation */
+	dmas = <&dmac 0 3>, <&dmac 1 4>;
+	dma-names = "rx", "tx";
+};
diff --git a/tests/drivers/uart/uart_async_api/src/test_uart.h b/tests/drivers/uart/uart_async_api/src/test_uart.h
index a55183715a79ff..74b7f2fb30cde9 100644
--- a/tests/drivers/uart/uart_async_api/src/test_uart.h
+++ b/tests/drivers/uart/uart_async_api/src/test_uart.h
@@ -23,6 +23,8 @@
 #define UART_DEVICE_NAME DT_UART_0_NAME
 #elif defined(CONFIG_BOARD_NRF9160DK_NRF9160)
 #define UART_DEVICE_NAME DT_UART_1_NAME
+#elif defined(CONFIG_BOARD_ATSAMD21_XPRO)
+#define UART_DEVICE_NAME DT_LABEL(DT_NODELABEL(sercom1))
 #else
 #define UART_DEVICE_NAME CONFIG_UART_CONSOLE_ON_DEV_NAME
 #endif