diff --git a/boards/nordic/nrf54h20dk/nrf54h20dk_nrf54h20-pinctrl.dtsi b/boards/nordic/nrf54h20dk/nrf54h20dk_nrf54h20-pinctrl.dtsi
index 1fb8a9ef2f13..79ac2c6b7ad7 100644
--- a/boards/nordic/nrf54h20dk/nrf54h20dk_nrf54h20-pinctrl.dtsi
+++ b/boards/nordic/nrf54h20dk/nrf54h20dk_nrf54h20-pinctrl.dtsi
@@ -26,12 +26,8 @@
 
 	/omit-if-no-ref/ uart135_default: uart135_default {
 		group1 {
-			psels = <NRF_PSEL(UART_TX, 1, 11)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(UART_RTS, 1, 9)>;
+			psels = <NRF_PSEL(UART_TX, 1, 11)>,
+				<NRF_PSEL(UART_RTS, 1, 9)>;
 		};
 
 		group3 {
@@ -53,12 +49,8 @@
 
 	/omit-if-no-ref/ uart136_default: uart136_default {
 		group1 {
-			psels = <NRF_PSEL(UART_TX, 2, 6)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(UART_RTS, 2, 7)>;
+			psels = <NRF_PSEL(UART_TX, 2, 6)>,
+				<NRF_PSEL(UART_RTS, 2, 7)>;
 		};
 
 		group3 {
diff --git a/dts/bindings/pinctrl/nordic,nrf-pinctrl.yaml b/dts/bindings/pinctrl/nordic,nrf-pinctrl.yaml
index fad0006d9447..304c1292e495 100644
--- a/dts/bindings/pinctrl/nordic,nrf-pinctrl.yaml
+++ b/dts/bindings/pinctrl/nordic,nrf-pinctrl.yaml
@@ -119,9 +119,3 @@ child-binding:
         description: |
           Invert pin polarity (set the active state to low).
           Only valid for PWM channel output pins.
-
-      nordic,clock-enable:
-        type: boolean
-        description: |
-          Enable clock for the pin. Only valid or required for certain
-          peripherals, refer to the reference manual.
diff --git a/tests/boards/nrf/dmm/boards/nrf54h20dk_nrf54h20_cpuapp.overlay b/tests/boards/nrf/dmm/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
index b65825bf4851..513ef21776ff 100644
--- a/tests/boards/nrf/dmm/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
+++ b/tests/boards/nrf/dmm/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
@@ -10,7 +10,6 @@
 		group1 {
 			psels = <NRF_PSEL(SPIM_MOSI, 2, 8)>,
 				<NRF_PSEL(SPIM_SCK, 1, 2)>;
-			nordic,clock-enable;
 		};
 	};
 
diff --git a/tests/boards/nrf/i2c/i2c_slave/boards/nrf54h20dk_nrf54h20_cpuapp.overlay b/tests/boards/nrf/i2c/i2c_slave/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
index 1906adc57e47..73d1d85cf453 100644
--- a/tests/boards/nrf/i2c/i2c_slave/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
+++ b/tests/boards/nrf/i2c/i2c_slave/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
@@ -9,7 +9,6 @@
 		group1 {
 			psels = <NRF_PSEL(TWIM_SDA, 2, 8)>,
 				<NRF_PSEL(TWIM_SCL, 1, 2)>;
-			nordic,clock-enable;
 		};
 	};
 
@@ -28,7 +27,6 @@
 			psels = <NRF_PSEL(TWIM_SDA, 2, 9)>,
 				<NRF_PSEL(TWIM_SCL, 1, 3)>;
 			bias-pull-up;
-			nordic,clock-enable;
 		};
 	};
 
diff --git a/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_common.dtsi b/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_common.dtsi
index 6aa412d48452..8661760a5e30 100644
--- a/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_common.dtsi
+++ b/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_common.dtsi
@@ -8,12 +8,8 @@
 	spi130_default_alt: spi130_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIM_SCK, 0, 0)>,
-				<NRF_PSEL(SPIM_MOSI, 0, 8)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIM_MISO, 0, 6)>;
+				<NRF_PSEL(SPIM_MOSI, 0, 8)>,
+				<NRF_PSEL(SPIM_MISO, 0, 6)>;
 		};
 	};
 
@@ -29,12 +25,8 @@
 	spis131_default_alt: spis131_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIS_SCK, 0, 1)>,
-				<NRF_PSEL(SPIS_MISO, 0, 7)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIS_MOSI, 0, 9)>,
+				<NRF_PSEL(SPIS_MISO, 0, 7)>,
+				<NRF_PSEL(SPIS_MOSI, 0, 9)>,
 				<NRF_PSEL(SPIS_CSN, 0, 11)>;
 		};
 	};
diff --git a/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay b/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay
index b16084da7862..0523426db458 100644
--- a/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay
+++ b/tests/drivers/spi/spi_controller_peripheral/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay
@@ -25,12 +25,8 @@
 	spis131_default_alt: spis131_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIS_SCK, 1, 0)>,
-				<NRF_PSEL(SPIS_MISO, 1, 9)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIS_MOSI, 1, 5)>,
+				<NRF_PSEL(SPIS_MISO, 1, 9)>,
+				<NRF_PSEL(SPIS_MOSI, 1, 5)>,
 				<NRF_PSEL(SPIS_CSN, 0, 11)>;
 		};
 	};
diff --git a/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp.overlay b/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
index 50b0428dcaa7..ec7e9a1702ae 100644
--- a/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
+++ b/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
@@ -8,12 +8,8 @@
 	spi130_default_alt: spi130_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIM_SCK, 0, 0)>,
-				<NRF_PSEL(SPIM_MOSI, 0, 8)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIM_MISO, 0, 6)>;
+				<NRF_PSEL(SPIM_MOSI, 0, 8)>,
+				<NRF_PSEL(SPIM_MISO, 0, 6)>;
 		};
 	};
 
@@ -29,12 +25,8 @@
 	spis131_default_alt: spis131_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIS_SCK, 0, 1)>,
-				<NRF_PSEL(SPIS_MISO, 0, 7)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIS_MOSI, 0, 9)>,
+				<NRF_PSEL(SPIS_MISO, 0, 7)>,
+				<NRF_PSEL(SPIS_MOSI, 0, 9)>,
 				<NRF_PSEL(SPIS_CSN, 0, 11)>;
 		};
 	};
diff --git a/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay b/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay
index b16084da7862..0523426db458 100644
--- a/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay
+++ b/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay
@@ -25,12 +25,8 @@
 	spis131_default_alt: spis131_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIS_SCK, 1, 0)>,
-				<NRF_PSEL(SPIS_MISO, 1, 9)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIS_MOSI, 1, 5)>,
+				<NRF_PSEL(SPIS_MISO, 1, 9)>,
+				<NRF_PSEL(SPIS_MOSI, 1, 5)>,
 				<NRF_PSEL(SPIS_CSN, 0, 11)>;
 		};
 	};
diff --git a/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuppr.overlay b/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuppr.overlay
index 1724d19ba285..7643b9419090 100644
--- a/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuppr.overlay
+++ b/tests/drivers/spi/spi_error_cases/boards/nrf54h20dk_nrf54h20_cpuppr.overlay
@@ -8,12 +8,8 @@
 	spi130_default_alt: spi130_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIM_SCK, 0, 0)>,
-				<NRF_PSEL(SPIM_MOSI, 0, 8)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIM_MISO, 0, 6)>;
+				<NRF_PSEL(SPIM_MOSI, 0, 8)>,
+				<NRF_PSEL(SPIM_MISO, 0, 6)>;
 		};
 	};
 
@@ -29,12 +25,8 @@
 	spis131_default_alt: spis131_default_alt {
 		group1 {
 			psels = <NRF_PSEL(SPIS_SCK, 0, 1)>,
-				<NRF_PSEL(SPIS_MISO, 0, 7)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIS_MOSI, 0, 9)>,
+				<NRF_PSEL(SPIS_MISO, 0, 7)>,
+				<NRF_PSEL(SPIS_MOSI, 0, 9)>,
 				<NRF_PSEL(SPIS_CSN, 0, 11)>;
 		};
 	};
diff --git a/tests/drivers/spi/spi_loopback/boards/nrf54h20dk_nrf54h20_cpuapp.overlay b/tests/drivers/spi/spi_loopback/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
index 3106fbf84533..89e5fbad08a9 100644
--- a/tests/drivers/spi/spi_loopback/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
+++ b/tests/drivers/spi/spi_loopback/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
@@ -8,12 +8,8 @@
 	spi130_default: spi130_default {
 		group1 {
 			psels = <NRF_PSEL(SPIM_SCK, 0, 0)>,
-				<NRF_PSEL(SPIM_MOSI, 0, 7)>;
-			nordic,clock-enable;
-		};
-
-		group2 {
-			psels = <NRF_PSEL(SPIM_MISO, 0, 6)>;
+				<NRF_PSEL(SPIM_MOSI, 0, 7)>,
+				<NRF_PSEL(SPIM_MISO, 0, 6)>;
 		};
 	};