From a367fdd99a028f7f7f1d4d55ddf0e4cdcba39d4b Mon Sep 17 00:00:00 2001 From: Kevin Lewis Date: Fri, 15 Mar 2019 10:56:49 +0000 Subject: [PATCH 01/23] update spatial_filter results parsing (bambi update) --- Changes | 2 ++ lib/npg_qc/autoqc/results/spatial_filter.pm | 2 +- 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/Changes b/Changes index 84f538d92..899c441cb 100644 --- a/Changes +++ b/Changes @@ -1,5 +1,7 @@ LIST OF CHANGES FOR NPG-QC PACKAGE + - update spatial_filter results parsing + release 66.3 - sequence_summary table - extend storage for the header column - remove fastqcheck database loader diff --git a/lib/npg_qc/autoqc/results/spatial_filter.pm b/lib/npg_qc/autoqc/results/spatial_filter.pm index 2e049421f..0c0b5150f 100644 --- a/lib/npg_qc/autoqc/results/spatial_filter.pm +++ b/lib/npg_qc/autoqc/results/spatial_filter.pm @@ -37,7 +37,7 @@ sub parse_output{ # this assumes the check is only run at lane level so id_run and position will be unique my $id_run = $self->composition->get_component(0)->id_run; my $position = $self->composition->get_component(0)->position; - my $key = $id_run . '_' . $position; + my $key = q[Lane ]. $position; my $num_total_reads = 0; my $num_spatial_filter_fail_reads = 0; From b5321f1555bd90b849263741796e5a02c042e3ec Mon Sep 17 00:00:00 2001 From: Kevin Lewis Date: Fri, 15 Mar 2019 14:18:33 +0000 Subject: [PATCH 02/23] correct key value for RE (use \s instead of literal space character) --- lib/npg_qc/autoqc/results/spatial_filter.pm | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/npg_qc/autoqc/results/spatial_filter.pm b/lib/npg_qc/autoqc/results/spatial_filter.pm index 0c0b5150f..e2f3dce10 100644 --- a/lib/npg_qc/autoqc/results/spatial_filter.pm +++ b/lib/npg_qc/autoqc/results/spatial_filter.pm @@ -37,7 +37,7 @@ sub parse_output{ # this assumes the check is only run at lane level so id_run and position will be unique my $id_run = $self->composition->get_component(0)->id_run; my $position = $self->composition->get_component(0)->position; - my $key = q[Lane ]. $position; + my $key = q[Lane\s]. $position; my $num_total_reads = 0; my $num_spatial_filter_fail_reads = 0; From 07c5c17e8b1e518d99346fa2f1742031b8f9e5a2 Mon Sep 17 00:00:00 2001 From: Kevin Lewis Date: Fri, 15 Mar 2019 14:19:57 +0000 Subject: [PATCH 03/23] update spatial_filter tests --- t/data/autoqc/25830_1#1.filter.stats | 3 +-- t/data/autoqc/25830_1#2.filter.stats | 2 +- t/data/autoqc/25830_1#3.filter.stats | 2 +- 3 files changed, 3 insertions(+), 4 deletions(-) diff --git a/t/data/autoqc/25830_1#1.filter.stats b/t/data/autoqc/25830_1#1.filter.stats index d2fbaf817..80d6dfd50 100644 --- a/t/data/autoqc/25830_1#1.filter.stats +++ b/t/data/autoqc/25830_1#1.filter.stats @@ -1,2 +1 @@ -25830_1#1 Processed 382658 Failed 32768 traces - +Lane 1 Processed 382658 Failed 32768 traces diff --git a/t/data/autoqc/25830_1#2.filter.stats b/t/data/autoqc/25830_1#2.filter.stats index 7d88854ed..b1b1f904e 100644 --- a/t/data/autoqc/25830_1#2.filter.stats +++ b/t/data/autoqc/25830_1#2.filter.stats @@ -1,2 +1,2 @@ -25830_1#2 Processed 306160 Failed 0 traces +Lane 1 Processed 306160 Failed 0 traces diff --git a/t/data/autoqc/25830_1#3.filter.stats b/t/data/autoqc/25830_1#3.filter.stats index a567558d7..c8712a649 100644 --- a/t/data/autoqc/25830_1#3.filter.stats +++ b/t/data/autoqc/25830_1#3.filter.stats @@ -1,2 +1,2 @@ -25830_1#3 Processed 54332 Failed 42 traces +Lane 1 Processed 54332 Failed 42 traces From 1bc4aef562f7d847f47d15042beb5c0531eab441 Mon Sep 17 00:00:00 2001 From: Kevin Lewis Date: Fri, 15 Mar 2019 14:29:57 +0000 Subject: [PATCH 04/23] tighten up key in RE ([ ] instead of \s) --- lib/npg_qc/autoqc/results/spatial_filter.pm | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/npg_qc/autoqc/results/spatial_filter.pm b/lib/npg_qc/autoqc/results/spatial_filter.pm index e2f3dce10..f13e6a98d 100644 --- a/lib/npg_qc/autoqc/results/spatial_filter.pm +++ b/lib/npg_qc/autoqc/results/spatial_filter.pm @@ -37,7 +37,7 @@ sub parse_output{ # this assumes the check is only run at lane level so id_run and position will be unique my $id_run = $self->composition->get_component(0)->id_run; my $position = $self->composition->get_component(0)->position; - my $key = q[Lane\s]. $position; + my $key = q{Lane[ ]}. $position; my $num_total_reads = 0; my $num_spatial_filter_fail_reads = 0; From 77dced61a3f9a69f3bcad3e23cb829795b6bb018 Mon Sep 17 00:00:00 2001 From: Carol Scott Date: Tue, 23 Apr 2019 11:06:47 +0100 Subject: [PATCH 05/23] add target autosome stats to bam_flagstats qc check --- Changes | 2 + MANIFEST | 2 + lib/npg_qc/Schema/Result/BamFlagstats.pm | 73 +++++++++++++++- lib/npg_qc/autoqc/checks/bam_flagstats.pm | 67 +++++++++----- lib/npg_qc/autoqc/results/bam_flagstats.pm | 9 +- lib/npg_qc/autoqc/role/bam_flagstats.pm | 34 ++++++++ .../root/src/ui_checks/bam_flagstats.tt2 | 51 +++++++++-- scripts/upgrade_schema/upgrade_schema-66.5 | 26 ++++++ t/60-autoqc-checks-bam_flagstats.t | 82 +++++++++++++++++- t/data/autoqc/bam_flagstats/25837_1_13.tar.gz | Bin 328498 -> 328539 bytes t/data/autoqc/bam_flagstats/29006_8_1.tar.gz | Bin 0 -> 402324 bytes 11 files changed, 314 insertions(+), 32 deletions(-) create mode 100644 scripts/upgrade_schema/upgrade_schema-66.5 create mode 100644 t/data/autoqc/bam_flagstats/29006_8_1.tar.gz diff --git a/Changes b/Changes index 0ecb7ec8d..5877670e6 100644 --- a/Changes +++ b/Changes @@ -1,5 +1,7 @@ LIST OF CHANGES FOR NPG-QC PACKAGE + - add target autosome stats to bam_flagstats qc check + release 66.4 - allow to finalise undecided library manual qc outcomes for all entities diff --git a/MANIFEST b/MANIFEST index aba24a4c9..21c869424 100644 --- a/MANIFEST +++ b/MANIFEST @@ -251,6 +251,8 @@ t/70-report-genotype_call.t t/80-autoqc-qc-script.t t/data/autoqc/bam_flagstats/16960_1_0.tar.gz t/data/autoqc/bam_flagstats/17448_1_9.tar.gz +t/data/autoqc/bam_flagstats/25837_1_13.tar.gz +t/data/autoqc/bam_flagstats/29006_8_1.tar.gz t/data/autoqc/bam_flagstats/24135_1#1.bam_flagstats.json t/data/autoqc/bam_flagstats/24135_1#1.flagstat t/data/autoqc/bam_flagstats/4783_5_bam_flagstats.json diff --git a/lib/npg_qc/Schema/Result/BamFlagstats.pm b/lib/npg_qc/Schema/Result/BamFlagstats.pm index e51f45a13..eaddcfe0e 100644 --- a/lib/npg_qc/Schema/Result/BamFlagstats.pm +++ b/lib/npg_qc/Schema/Result/BamFlagstats.pm @@ -264,6 +264,61 @@ The coverage threshold used in the perc target greater than depth calculation The percentage of the target covered at greater than the depth specified +=head2 target_autosome_filter + + data_type: 'varchar' + is_nullable: 1 + size: 30 + +Filter used to produce the target autosome stats file + +=head2 target_autosome_length + + data_type: 'bigint' + extra: {unsigned => 1} + is_nullable: 1 + +The total length of the target autosome regions + +=head2 target_autosome_mapped_reads + + data_type: 'bigint' + extra: {unsigned => 1} + is_nullable: 1 + +The number of mapped reads passing the filter + +=head2 target_autosome_proper_pair_mapped_reads + + data_type: 'bigint' + extra: {unsigned => 1} + is_nullable: 1 + +The number of proper pair mapped reads passing the filter + +=head2 target_autosome_mapped_bases + + data_type: 'bigint' + extra: {unsigned => 1} + is_nullable: 1 + +The number of mapped bases passing the filter + +=head2 target_autosome_coverage_threshold + + data_type: 'integer' + is_nullable: 1 + +The coverage threshold used in the perc target autosome greater than depth calculation + +=head2 target_autosome_percent_gt_coverage_threshold + + data_type: 'float' + is_nullable: 1 + size: [5,2] + +The percentage of the target autosome covered at greater than the depth specified + =cut __PACKAGE__->add_columns( @@ -343,6 +398,20 @@ __PACKAGE__->add_columns( { data_type => 'integer', is_nullable => 1 }, 'target_percent_gt_coverage_threshold', { data_type => 'float', is_nullable => 1, size => [5, 2] }, + 'target_autosome_filter', + { data_type => 'varchar', is_nullable => 1, size => 30 }, + 'target_autosome_length', + { data_type => 'bigint', extra => { unsigned => 1 }, is_nullable => 1 }, + 'target_autosome_mapped_reads', + { data_type => 'bigint', extra => { unsigned => 1 }, is_nullable => 1 }, + 'target_autosome_proper_pair_mapped_reads', + { data_type => 'bigint', extra => { unsigned => 1 }, is_nullable => 1 }, + 'target_autosome_mapped_bases', + { data_type => 'bigint', extra => { unsigned => 1 }, is_nullable => 1 }, + 'target_autosome_coverage_threshold', + { data_type => 'integer', is_nullable => 1 }, + 'target_autosome_percent_gt_coverage_threshold', + { data_type => 'float', is_nullable => 1, size => [5, 2] }, ); =head1 PRIMARY KEY @@ -408,8 +477,8 @@ __PACKAGE__->belongs_to( with 'npg_qc::Schema::Composition', 'npg_qc::Schema::Flators', 'npg_qc::autoqc::role::result', 'npg_qc::autoqc::role::bam_flagstats'; -# Created by DBIx::Class::Schema::Loader v0.07049 @ 2018-06-14 16:17:33 -# DO NOT MODIFY THIS OR ANYTHING ABOVE! md5sum:7FncD4g/I8dgGfG9B9a0CQ +# Created by DBIx::Class::Schema::Loader v0.07049 @ 2019-04-17 14:56:54 +# DO NOT MODIFY THIS OR ANYTHING ABOVE! md5sum:YsrYNzYhK4jAltTpq12t+g # You can replace this text with custom code or comments, and it will be preserved on regeneration diff --git a/lib/npg_qc/autoqc/checks/bam_flagstats.pm b/lib/npg_qc/autoqc/checks/bam_flagstats.pm index 2561ee872..9b0222e11 100644 --- a/lib/npg_qc/autoqc/checks/bam_flagstats.pm +++ b/lib/npg_qc/autoqc/checks/bam_flagstats.pm @@ -34,7 +34,8 @@ Readonly::Hash my %METRICS_FIELD_MAPPING => { Readonly::Scalar my $LIBRARY_SIZE_NOT_AVAILABLE => -1; Readonly::Scalar my $METRICS_NUMBER => 9; Readonly::Scalar my $PAIR_NUMBER => 2; -Readonly::Scalar my $TARGET_STATS_PATTERN => '_target'; +Readonly::Scalar my $TARGET_STATS_PATTERN => 'target'; +Readonly::Scalar my $TARGET_AUTOSOME_STATS_PATTERN => 'target_autosome'; Readonly::Scalar my $TARGET_STATS_DEFAULT_DEPTH => 15; Readonly::Scalar my $STATS_FILTER => '[[:alnum:]]+[\_[:lower:]]*?'; Readonly::Scalar our $EXT => q[bam]; @@ -115,13 +116,16 @@ has 'target_stats_file' => ( isa => 'Str | Undef', ); sub _build_target_stats_file { my $self = shift; - my $file; - if($self->samtools_stats_file && @{$self->samtools_stats_file}){ - my @x = @{$self->samtools_stats_file}; - my @found = grep { /$TARGET_STATS_PATTERN/smx } @{$self->samtools_stats_file}; - if (@found == 1){ $file = $found[0]; } - } - return $file; + return $self->_find_stats_file($TARGET_STATS_PATTERN); +} +has 'target_autosome_stats_file' => ( isa => 'Str | Undef', + is => 'ro', + lazy_build => 1, +); + +sub _build_target_autosome_stats_file { + my $self = shift; + return $self->_find_stats_file($TARGET_AUTOSOME_STATS_PATTERN); } has 'related_results' => ( isa => 'ArrayRef[Object]', @@ -156,7 +160,12 @@ override 'execute' => sub { $self->_parse_markdups_metrics(); } if( $self->target_stats_file ) { - $self->_parse_target_stats_file(); + $self->_parse_target_stats_file + ($self->target_stats_file,$TARGET_STATS_PATTERN); + } + if( $self->target_autosome_stats_file ) { + $self->_parse_target_stats_file + ($self->target_autosome_stats_file,$TARGET_AUTOSOME_STATS_PATTERN); } $self->_parse_flagstats(); for my $rr ( @{$self->related_results()} ) { @@ -246,32 +255,34 @@ sub _parse_flagstats { } sub _parse_target_stats_file { - my $self = shift; + my($self,$fn,$prefix) = @_; - my $fn = $self->target_stats_file; ## no critic (InputOutput::RequireBriefOpen) open my $target_stats_fh, '<', $fn or croak "Error: $OS_ERROR - failed to open $fn for reading"; while ( my $line = <$target_stats_fh> ) { chomp $line; if ( $line =~ /The\ command\ line\ was/mxs ) { - if( my ($td) = $line =~ /-[g|cov\-threshold]\s*(\d+)/mxs){ - $self->result()->target_coverage_threshold($td); + if($line =~ /-(g|cov\-threshold)\s*(\d+)/mxs ){ + $self->_set_result($prefix . '_coverage_threshold',$2); } else{ - $self->result()->target_coverage_threshold($TARGET_STATS_DEFAULT_DEPTH); + $self->_set_result($prefix . '_coverage_threshold',$TARGET_STATS_DEFAULT_DEPTH); + } + if( $line =~ /-(t|target\-regions)\s*([\w\/\-\.]+)/mxs ){ + $self->result()->set_info($prefix .'_path', $2); } } elsif ( $line =~ /^SN\s+/mxs ){ my ($number) = $line =~ /^SN\s+.*\:\s+([\d\.]+)\b/mxs; ( $line =~ /reads\ mapped\:/mxs ) - ? $self->result()->target_mapped_reads($number) + ? $self->_set_result($prefix . '_mapped_reads',$number) : ( $line =~ /reads\ properly\ paired\:/mxs ) - ? $self->result()->target_proper_pair_mapped_reads($number) + ? $self->_set_result($prefix . '_proper_pair_mapped_reads',$number) : ( $line =~ /bases\ mapped\ \(cigar\)\:/mxs ) - ? $self->result()->target_mapped_bases($number) + ? $self->_set_result($prefix . '_mapped_bases',$number) : ( $line =~ /bases\ inside\ the\ target\:/mxs ) - ? $self->result()->target_length($number) + ? $self->_set_result($prefix . '_length',$number) : ( $line =~ /percentage\ of\ target\ genome\ with\ coverage/mxs ) - ? $self->result()->target_percent_gt_coverage_threshold($number) + ? $self->_set_result($prefix . '_percent_gt_coverage_threshold',$number) : next; } elsif ( $line =~ /^FFQ\s+/mxs ) { last; } @@ -279,11 +290,27 @@ sub _parse_target_stats_file { close $target_stats_fh or carp "Warning: $OS_ERROR - failed to close filehandle to $fn"; my ($filter) = $fn =~ /_($STATS_FILTER)[.]stats\Z/xms; - $self->result()->target_filter($filter); + $self->_set_result($prefix . '_filter',$filter); return; } +sub _set_result { + my ($self,$method,$result) = @_; + $self->result()->$method($result); + return; +} + +sub _find_stats_file { + my ($self,$pattern) = @_; + my $file; + if($self->samtools_stats_file && @{$self->samtools_stats_file}){ + my @x = @{$self->samtools_stats_file}; + my @found = grep { /\_$pattern\./smx } @{$self->samtools_stats_file}; + if (@found == 1){ $file = $found[0]; } + } + return $file; +} __PACKAGE__->meta->make_immutable; diff --git a/lib/npg_qc/autoqc/results/bam_flagstats.pm b/lib/npg_qc/autoqc/results/bam_flagstats.pm index bcbb7d585..bf0105e7b 100644 --- a/lib/npg_qc/autoqc/results/bam_flagstats.pm +++ b/lib/npg_qc/autoqc/results/bam_flagstats.pm @@ -15,6 +15,7 @@ our $VERSION = '0'; has [ qw/ library target_filter + target_autosome_filter / ] => ( isa => 'Maybe[Str]', is => 'rw', @@ -36,13 +37,19 @@ has [ qw/ num_total_reads target_proper_pair_mapped_reads target_mapped_bases target_coverage_threshold - / ] => ( + target_autosome_length + target_autosome_mapped_reads + target_autosome_proper_pair_mapped_reads + target_autosome_mapped_bases + target_autosome_coverage_threshold + / ] => ( isa => 'Maybe[Int]', is => 'rw', ); has [ qw/ percent_duplicate target_percent_gt_coverage_threshold + target_autosome_percent_gt_coverage_threshold / ] => ( isa => 'Maybe[Num]', is => 'rw', diff --git a/lib/npg_qc/autoqc/role/bam_flagstats.pm b/lib/npg_qc/autoqc/role/bam_flagstats.pm index 5890dd1b4..6c4617a40 100644 --- a/lib/npg_qc/autoqc/role/bam_flagstats.pm +++ b/lib/npg_qc/autoqc/role/bam_flagstats.pm @@ -76,6 +76,34 @@ sub percent_target_proper_pair_mapped_reads { return; } +sub target_mean_coverage { + my $self = shift; + if (defined $self->target_mapped_bases && defined $self->target_length) { + return $self->target_mapped_bases / $self->target_length; + } + return; +} + +sub percent_target_autosome_proper_pair_mapped_reads { + my $self = shift; + if (defined $self->target_autosome_mapped_reads && + defined $self->target_autosome_proper_pair_mapped_reads) { + return $PERCENTAGE * + $self->target_autosome_proper_pair_mapped_reads / $self->target_autosome_mapped_reads; + } + return; +} + +sub target_autosome_mean_coverage { + my $self = shift; + if (defined $self->target_autosome_mapped_bases && defined $self->target_autosome_length) { + return $self->target_autosome_mapped_bases / $self->target_autosome_length; + } + return; +} + + + no Moose; 1; @@ -101,8 +129,14 @@ __END__ =head2 percent_singletons +=head2 percent_target_autosome_proper_pair_mapped_reads + =head2 percent_target_proper_pair_mapped_reads +=head2 target_autosome_mean_coverage + +=head2 target_mean_coverage + =head2 total_duplicate_reads =head2 total_mapped_reads diff --git a/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 b/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 index 34b64ded4..40760720e 100644 --- a/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 +++ b/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 @@ -48,28 +48,63 @@ Flagstats [% IF check.target_length.defined && check.target_length > 0 %] Target region stats - - + + - + - + - + - + - + + + - + [% END %]
Target stats descriptionValue
target filter
Stats descriptionValue
filter [% IF check.target_filter.defined; check.target_filter ; END %]
target regions length (bp)
regions length (bp) [% IF check.target_length.defined; check.target_length | format_number; END %]
target mapped reads
mapped reads [% IF check.target_mapped_reads.defined; check.target_mapped_reads | format_number; END %]
target proper pair mapped reads
proper pair mapped reads [% IF check.target_proper_pair_mapped_reads.defined; check.target_proper_pair_mapped_reads | format_number; END %]
% target mapped reads which are mapped in proper pairs
% mapped reads which are mapped in proper pairs [% IF check.percent_target_proper_pair_mapped_reads.defined; check.percent_target_proper_pair_mapped_reads | format('%3.2f') ; END %]
yield within target (bp)[% IF check.target_mapped_bases.defined; check.target_mapped_bases | format_number ; END %]
[% IF check.target_mapped_bases.defined; check.target_mapped_bases | format_number ; END %]
mean coverage (X)[% IF check.target_mean_coverage.defined; check.target_mean_coverage | format_number ; END %]
coverage threshold (X) [% IF check.target_coverage_threshold.defined; check.target_coverage_threshold | format_number ; END %]
% target regions above coverage threshold
% regions above coverage threshold [% IF check.target_percent_gt_coverage_threshold.defined; check.target_percent_gt_coverage_threshold | format('%3.2f'); END %]
+ +     + + +
+ +[% IF check.target_autosome_length.defined && check.target_autosome_length > 0 %] +Autosomes only target region stats + + + + + + + + + + + + + + + + + + + +[% END %] +
Stats descriptionValue
filter[% IF check.target_autosome_filter.defined; check.target_autosome_filter ; END %]
regions length (bp)[% IF check.target_autosome_length.defined; check.target_autosome_length | format_number; END %]
mapped reads[% IF check.target_autosome_mapped_reads.defined; check.target_autosome_mapped_reads | format_number; END %]
proper pair mapped reads [% IF check.target_autosome_proper_pair_mapped_reads.defined; check.target_autosome_proper_pair_mapped_reads | format_number; END %]
% mapped reads which are mapped in proper pairs[% IF check.percent_target_autosome_proper_pair_mapped_reads.defined; check.percent_target_autosome_proper_pair_mapped_reads | format('%3.2f') ; END %]
yield within target (bp)[% IF check.target_autosome_mapped_bases.defined; check.target_autosome_mapped_bases | format_number ; END %]
mean coverage (X)[% IF check.target_autosome_mean_coverage.defined; check.target_autosome_mean_coverage | format_number ; END %]
coverage threshold (X)[% IF check.target_autosome_coverage_threshold.defined; check.target_autosome_coverage_threshold | format_number ; END %]
% regions above coverage threshold[% IF check.target_autosome_percent_gt_coverage_threshold.defined; check.target_autosome_percent_gt_coverage_threshold | format('%3.2f'); END %]
+ +
+ + diff --git a/scripts/upgrade_schema/upgrade_schema-66.5 b/scripts/upgrade_schema/upgrade_schema-66.5 new file mode 100644 index 000000000..a43ab4855 --- /dev/null +++ b/scripts/upgrade_schema/upgrade_schema-66.5 @@ -0,0 +1,26 @@ +-- +-- add columns to the bam_flagstats table +-- + +ALTER TABLE `bam_flagstats` \ +ADD COLUMN `target_autosome_filter` varchar(30) DEFAULT NULL \ + COMMENT 'Filter used to produce the target autosome stats file' \ + AFTER `target_percent_gt_coverage_threshold`, +ADD COLUMN `target_autosome_length` bigint(12) unsigned DEFAULT NULL \ + COMMENT 'The total length of the target autosome regions' \ + AFTER `target_autosome_filter`, +ADD COLUMN `target_autosome_mapped_reads` bigint(20) unsigned DEFAULT NULL \ + COMMENT 'The number of mapped reads passing the filter' \ + AFTER `target_autosome_length`, +ADD COLUMN `target_autosome_proper_pair_mapped_reads` bigint(20) unsigned DEFAULT NULL \ + COMMENT 'The number of proper pair mapped reads passing the filter' \ + AFTER `target_autosome_mapped_reads`, +ADD COLUMN `target_autosome_mapped_bases` bigint(20) unsigned DEFAULT NULL \ + COMMENT 'The number of mapped bases passing the filter' \ + AFTER `target_autosome_proper_pair_mapped_reads`, +ADD COLUMN `target_autosome_coverage_threshold` int(4) DEFAULT NULL \ + COMMENT 'The coverage threshold used in the perc target autosome greater than depth calculation' \ + AFTER `target_autosome_mapped_bases`, +ADD COLUMN `target_autosome_percent_gt_coverage_threshold` float(5,2) DEFAULT NULL \ + COMMENT 'The percentage of the target autosome covered at greater than the depth specified' \ + AFTER `target_autosome_coverage_threshold`; diff --git a/t/60-autoqc-checks-bam_flagstats.t b/t/60-autoqc-checks-bam_flagstats.t index 026a5eef3..7746f51ba 100644 --- a/t/60-autoqc-checks-bam_flagstats.t +++ b/t/60-autoqc-checks-bam_flagstats.t @@ -1,6 +1,6 @@ use strict; use warnings; -use Test::More tests => 10; +use Test::More tests => 11; use Test::Exception; use Test::Deep; use File::Temp qw( tempdir ); @@ -482,4 +482,84 @@ subtest 'full functionality with optional target stats' => sub { }; +my $archive_29006 = '29006_8_1'; +my $ae_29006 = Archive::Extract->new(archive => "t/data/autoqc/bam_flagstats/${archive_29006}.tar.gz"); +$ae_29006->extract(to => $tempdir) or die $ae_29006->error; +$archive_29006 = join q[/], $tempdir, $archive_29006; +my $qc_dir_29006 = join q[/], $archive_29006, 'testqc1'; +mkdir $qc_dir_29006; + + +subtest 'full functionality with optional target and autosome target stats' => sub { + + plan tests => 24; + + my $fproot_common = $archive_29006 . '/29006_8#1'; + my $composition_digest = '46fe9f6fffc7f2d7a74c4764f62103d1bcf9b8edf8ae6d4991f9330efd45c7f9'; + + foreach my $file_type ( qw(cram bam) ) { + + my $local_qc_dir = join q[/], $qc_dir_29006, $file_type; + if (!-e $local_qc_dir) { + mkdir $local_qc_dir; + } + + my $ref = { + id_run => 29006, + position => 8, + tag_index => 1, + qc_out => $local_qc_dir, + }; + + my $sfile = join q[.], $fproot_common, $file_type; + + if ($file_type eq 'bam') { + $ref->{'input_files'} = [$sfile]; + } else { + $ref->{'qc_in'} = $archive_29006; + } + + my $r = npg_qc::autoqc::checks::bam_flagstats->new($ref); + lives_ok { $r->run() } 'no error calling run()'; + + my @ros = @{$r->related_results}; + is (scalar @ros, 5, 'five related results'); + + my $ro = $ros[4]; + isa_ok ($ro, 'npg_qc::autoqc::results::sequence_summary'); + isa_ok ($ro->composition, 'npg_tracking::glossary::composition'); + is ($ro->composition_digest, $composition_digest, 'composition digest'); + + foreach my $output_type ( qw(.bam_flagstats.json + .sequence_summary.json + _F0xB00.samtools_stats.json + _F0x900.samtools_stats.json + _F0xF04_target.samtools_stats.json + _F0xF04_target_autosome.samtools_stats.json + ) ) { + my @dirs = splitdir $fproot_common; + my $name = pop @dirs; + my $output = catdir($local_qc_dir, $name) . $output_type; + ok (-e $output, "output $output created"); + } + + my $expected_from_json = from_json( + slurp qq{$archive_29006/qc/all_json/29006_8#1.bam_flagstats.json}, {chomp=>1}); + + my $results_from_json = from_json( + slurp qq{$local_qc_dir/29006_8#1.bam_flagstats.json}, {chomp=>1}); + + foreach my $res ($expected_from_json, $results_from_json){ + delete $res->{'__CLASS__'}; + delete $res->{'composition'}; + delete $res->{'info'}->{'Check'}; + delete $res->{'info'}->{'Check_version'}; + delete $res->{'path'}; + } + + is_deeply($results_from_json, $expected_from_json, 'correct json output'); + } + +}; + 1; diff --git a/t/data/autoqc/bam_flagstats/25837_1_13.tar.gz 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lib/npg_qc/autoqc/role/bam_flagstats.pm | 14 ++++++++ npg_qc_viewer/root/src/about_qc_checks.tt2 | 27 ++++++++++++++-- .../root/src/ui_checks/bam_flagstats.tt2 | 2 +- .../root/src/ui_lanes/checks_header.tt2 | 4 +-- npg_qc_viewer/root/src/ui_lanes/lane.tt2 | 32 +++++++++++++++++-- 5 files changed, 70 insertions(+), 9 deletions(-) diff --git a/lib/npg_qc/autoqc/role/bam_flagstats.pm b/lib/npg_qc/autoqc/role/bam_flagstats.pm index 6c4617a40..9d0025665 100644 --- a/lib/npg_qc/autoqc/role/bam_flagstats.pm +++ b/lib/npg_qc/autoqc/role/bam_flagstats.pm @@ -6,6 +6,9 @@ use Readonly; our $VERSION = '0'; Readonly::Scalar my $PERCENTAGE => 100; +Readonly::Scalar my $TWO_PLACES => 2; +Readonly::Scalar my $FACTOR_TEN => 10; +Readonly::Scalar my $GIGABASE => 1_000_000_000; sub total_reads { my $self = shift; @@ -84,6 +87,15 @@ sub target_mean_coverage { return; } +sub target_mapped_bases_gb{ + my $self = shift; + if (defined $self->target_mapped_bases) { + my $factor = $FACTOR_TEN**$TWO_PLACES; + return int(($self->target_mapped_bases/$GIGABASE) * $factor) / $factor; + } + return; +} + sub percent_target_autosome_proper_pair_mapped_reads { my $self = shift; if (defined $self->target_autosome_mapped_reads && @@ -139,6 +151,8 @@ __END__ =head2 total_duplicate_reads +=head2 target_mapped_bases_gb + =head2 total_mapped_reads =head2 total_reads diff --git a/npg_qc_viewer/root/src/about_qc_checks.tt2 b/npg_qc_viewer/root/src/about_qc_checks.tt2 index ae4d5fddb..0de3fe609 100644 --- a/npg_qc_viewer/root/src/about_qc_checks.tt2 +++ b/npg_qc_viewer/root/src/about_qc_checks.tt2 @@ -198,6 +198,7 @@ Metrics generated as part of calling a set of genotypes for a sample and compari

RNA-SeQC metrics

+

Description

Subset of metrics obtained from Broad Institute's RNA-SeQC package. The following summary statistics are calculated by counting the number of reads that have the given characteristics.


@@ -216,8 +217,9 @@ Metrics generated as part of calling a set of genotypes for a sample and compari
-

Tag metrics

-

Description

+

Tag metrics

+
+

Description

This check displays statistics about decoding of the index read. The semantics of the summary table data differs for pools and individual libraries.

For a pool the overall decoding percent is displayed, followed by the coefficient of variance that characterises the uniformity of tag distribution in a pool. The background of the table cell is blue if the overall decoding rate is over 80%, for lesser values the background is red.

For an individual library (tag), the decoding percent for this library within a pool is displayed. If the number of reads for this tag is at least one tenth the average number of reads per tag for this pool, the background is grey; otherwise, the background is red.

@@ -231,12 +233,31 @@ Metrics generated as part of calling a set of genotypes for a sample and compari

Upstream Tags Check

-

Description

+
+

Description

Check for contamination of tag#0 BAM file by reads from upstream runs


+ +

bam flagstats check

+
+

Description

+The bam flagstats check reports simple read stats. Optionally there are +also target regions stats and autosome only target regions stats where +appropriate regions sets are available. +

+The header table lists percentage of reads which are mapped, percentage of +reads which are duplicates if available and optionally the following three +target metrics separated by a pipe delimiter ;

+ + + + + +
FieldDescription
PP%% mapped reads which are mapped in proper pairs - from Target region stats
Gbyield within target (bp) - from Target region stats
Cov% regions above coverage threshold - from Autosomes only target region stats
+

VerifyBamID contamination check



diff --git a/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 b/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 index 40760720e..33c2f6019 100644 --- a/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 +++ b/npg_qc_viewer/root/src/ui_checks/bam_flagstats.tt2 @@ -58,7 +58,7 @@ Target region stats proper pair mapped reads [% IF check.target_proper_pair_mapped_reads.defined; check.target_proper_pair_mapped_reads | format_number; END %] % mapped reads which are mapped in proper pairs - [% IF check.percent_target_proper_pair_mapped_reads.defined; check.percent_target_proper_pair_mapped_reads | format('%3.2f') ; END %] + [% IF check.percent_target_proper_pair_mapped_reads.defined; check.percent_target_proper_pair_mapped_reads | format('%3.2f') ; END %] yield within target (bp) [% IF check.target_mapped_bases.defined; check.target_mapped_bases | format_number ; END %] mean coverage (X) diff --git a/npg_qc_viewer/root/src/ui_lanes/checks_header.tt2 b/npg_qc_viewer/root/src/ui_lanes/checks_header.tt2 index 4d7757c0a..97d4d6f61 100644 --- a/npg_qc_viewer/root/src/ui_lanes/checks_header.tt2 +++ b/npg_qc_viewer/root/src/ui_lanes/checks_header.tt2 @@ -2,7 +2,7 @@ labels = { adapter = 'adapters,
%' alignment_filter_metrics = 'target, %' - bam_flagstats = 'mapped %
duplicates %' + bam_flagstats = 'mapped %
duplicates %
PP %|Gb|Cov' bcfstats = 'concordance %
NRD %
' contamination = 'top two' gc_bias = 'plot created
' @@ -30,7 +30,7 @@ [% IF (check_name.match('genotype') && !check_name.match('call')) && !check_name.match('W30467') %] [% NEXT %] [% END %] - [% IF check_name.match('genotype') || check_name == "bcfstats"|| check_name == "rna seqc" || check_name == "upstream tags" || check_name == "verify bam id" || check_name.match('tag metrics') %] + [% IF check_name.match('genotype') || check_name == "bam flagstats" || check_name == "bcfstats" || check_name == "rna seqc" || check_name == "upstream tags" || check_name == "verify bam id" || check_name.match('tag metrics') %] [% info_button='
Link to information about ' _ check_name _ ' check' %] [% ELSE %] [% IF check_name == 'alignment filter metrics'; check_name = 'alignment filter'; END %] diff --git a/npg_qc_viewer/root/src/ui_lanes/lane.tt2 b/npg_qc_viewer/root/src/ui_lanes/lane.tt2 index 1ff2f96e8..18afb1cfc 100644 --- a/npg_qc_viewer/root/src/ui_lanes/lane.tt2 +++ b/npg_qc_viewer/root/src/ui_lanes/lane.tt2 @@ -256,9 +256,35 @@ [%- END -%]
[%- duplicate_rate = result.percent_duplicate_reads -%] [%- IF duplicate_rate.defined && duplicate_rate -%] - [% FILTER format('%2.2f'); duplicate_rate; END %] -[%- END -%] -[%- END -%] + [% FILTER format('%2.2f'); duplicate_rate; END %] +[%- END -%]
+ +[%- target_pp = result.percent_target_proper_pair_mapped_reads -%] +[%- IF target_pp.defined && target_pp != '' -%] + +[%- IF target_pp.defined && target_pp != '' -%] +[% FILTER format('%2.2f'); target_pp; END %] +[%- ELSE -%] +- +[%- END -%] +| +[%- target_yield = result.target_mapped_bases_gb -%] +[%- IF target_yield.defined && target_yield != '' -%] +[% FILTER format('%.2f'); target_yield; END %] +[%- ELSE -%] +- +[%- END -%] +| +[%- target_agt = result.target_autosome_percent_gt_coverage_threshold -%] +[%- IF target_agt.defined && target_agt != '' -%] +[% FILTER format('%2.2f'); target_agt; END %] +[%- ELSE -%] +- +[%- END -%] + +[%- END -%] +[%- END -%] + [% BLOCK tags_reporters -%] [% href='#trc_' _ result.id_run _ ':' _ result.position %] From 6b7c998e5d818b2b94cdbc879920c9d8454d1210 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Tue, 21 May 2019 12:29:07 +0100 Subject: [PATCH 07/23] scaffold for a autoqc meta check to underpin roboqc process --- Build.PL | 1 + MANIFEST | 14 + lib/npg_qc/autoqc/checks/meta.pm | 394 ++++++++++++++++++ lib/npg_qc/autoqc/results/meta.pm | 109 +++++ t/60-autoqc-checks-meta.t | 179 ++++++++ t/60-autoqc-results-meta.t | 8 + .../autoqc/meta/29524#17.bam_flagstats.json | 1 + t/data/autoqc/meta/29524#2.bam_flagstats.json | 1 + t/data/autoqc/meta/29524#2.bcfstats.json | 1 + t/data/autoqc/meta/29524#2.qX_yield.json | 1 + t/data/autoqc/meta/29524#2.verify_bam_id.json | 1 + .../meta/29524#2_phix.bam_flagstats.json | 1 + t/data/autoqc/meta/29524#3.bam_flagstats.json | 1 + t/data/autoqc/meta/29524#7.bam_flagstats.json | 1 + t/data/autoqc/meta/error1/product_release.yml | 37 ++ t/data/autoqc/meta/error2/product_release.yml | 37 ++ .../meta/no_criteria/product_release.yml | 36 ++ .../autoqc/meta/no_robo/product_release.yml | 24 ++ t/data/autoqc/meta/product_release.yml | 34 ++ t/data/autoqc/meta/samplesheet_27483.csv | 1 + .../meta/with_criteria/product_release.yml | 37 ++ 21 files changed, 919 insertions(+) create mode 100644 lib/npg_qc/autoqc/checks/meta.pm create mode 100644 lib/npg_qc/autoqc/results/meta.pm create mode 100644 t/60-autoqc-checks-meta.t create mode 100644 t/60-autoqc-results-meta.t create mode 100644 t/data/autoqc/meta/29524#17.bam_flagstats.json create mode 100644 t/data/autoqc/meta/29524#2.bam_flagstats.json create mode 100644 t/data/autoqc/meta/29524#2.bcfstats.json create mode 100644 t/data/autoqc/meta/29524#2.qX_yield.json create mode 100644 t/data/autoqc/meta/29524#2.verify_bam_id.json create mode 100644 t/data/autoqc/meta/29524#2_phix.bam_flagstats.json create mode 100644 t/data/autoqc/meta/29524#3.bam_flagstats.json create mode 100644 t/data/autoqc/meta/29524#7.bam_flagstats.json create mode 100644 t/data/autoqc/meta/error1/product_release.yml create mode 100644 t/data/autoqc/meta/error2/product_release.yml create mode 100644 t/data/autoqc/meta/no_criteria/product_release.yml create mode 100644 t/data/autoqc/meta/no_robo/product_release.yml create mode 100644 t/data/autoqc/meta/product_release.yml create mode 120000 t/data/autoqc/meta/samplesheet_27483.csv create mode 100644 t/data/autoqc/meta/with_criteria/product_release.yml diff --git a/Build.PL b/Build.PL index a4bc8133a..d9c2244d9 100644 --- a/Build.PL +++ b/Build.PL @@ -98,6 +98,7 @@ my $requires = { 'npg_tracking::illumina::runfolder' => 0, 'npg_tracking::Schema' => 0, 'npg_tracking::util::abs_path' => 0, + 'npg_tracking::util::pipeline_config' => 0, 'npg_tracking::util::types' => 0, 'npg::util' => 0, 'st::api::base' => 0, diff --git a/MANIFEST b/MANIFEST index aba24a4c9..abd4fdc37 100644 --- a/MANIFEST +++ b/MANIFEST @@ -28,6 +28,7 @@ lib/npg_qc/autoqc/checks/gc_fraction.pm lib/npg_qc/autoqc/checks/genotype.pm lib/npg_qc/autoqc/checks/genotype_call.pm lib/npg_qc/autoqc/checks/insert_size.pm +lib/npg_qc/autoqc/checks/meta.pm lib/npg_qc/autoqc/checks/pulldown_metrics.pm lib/npg_qc/autoqc/checks/qX_yield.pm lib/npg_qc/autoqc/checks/ref_match.pm @@ -58,6 +59,7 @@ lib/npg_qc/autoqc/results/gc_fraction.pm lib/npg_qc/autoqc/results/genotype.pm lib/npg_qc/autoqc/results/genotype_call.pm lib/npg_qc/autoqc/results/insert_size.pm +lib/npg_qc/autoqc/results/meta.pm lib/npg_qc/autoqc/results/pulldown_metrics.pm lib/npg_qc/autoqc/results/qX_yield.pm lib/npg_qc/autoqc/results/ref_match.pm @@ -204,6 +206,7 @@ t/60-autoqc-checks-gc_fraction.t t/60-autoqc-checks-genotype.t t/60-autoqc-checks-genotype_call.t t/60-autoqc-checks-insert_size.t +t/60-autoqc-checks-meta.t t/60-autoqc-checks-pulldown_metrics.t t/60-autoqc-checks-qX_yield.t t/60-autoqc-checks-ref_match.t @@ -232,6 +235,7 @@ t/60-autoqc-results-gc_fraction.t t/60-autoqc-results-genotype.t t/60-autoqc-results-genotype_call.t t/60-autoqc-results-insert_size.t +t/60-autoqc-results-meta.t t/60-autoqc-results-pulldown_metrics.t t/60-autoqc-results-qX_yield.t t/60-autoqc-results-ref_match.t @@ -418,6 +422,16 @@ t/data/autoqc/load/3510_1_1_human_split_stats.json t/data/autoqc/load/3510_5.qX_yield.json t/data/autoqc/load/do_not_add_files_to_this_dir t/data/autoqc/load/some.json +t/data/autoqc/meta/product_release.yml +t/data/autoqc/meta/samplesheet_27483.csv +t/data/autoqc/meta/29524#17.bam_flagstats.json +t/data/autoqc/meta/29524#2.bam_flagstats.json +t/data/autoqc/meta/29524#2.bcfstats.json +t/data/autoqc/meta/29524#2.qX_yield.json +t/data/autoqc/meta/29524#2.verify_bam_id.json +t/data/autoqc/meta/29524#2_phix.bam_flagstats.json +t/data/autoqc/meta/29524#3.bam_flagstats.json +t/data/autoqc/meta/29524#7.bam_flagstats.json t/data/autoqc/npg/instrument/15.xml t/data/autoqc/npg/instrument/42.xml t/data/autoqc/npg/run/1937.xml diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/meta.pm new file mode 100644 index 000000000..1edf0df6f --- /dev/null +++ b/lib/npg_qc/autoqc/checks/meta.pm @@ -0,0 +1,394 @@ +package npg_qc::autoqc::checks::meta; + +use Moose; +use namespace::autoclean; +use Carp; +use Readonly; +use List::MoreUtils qw/any uniq/; + +use st::api::lims; +use npg_qc::autoqc::qc_store; + +extends 'npg_qc::autoqc::checks::check'; +with 'npg_tracking::util::pipeline_config'; + +our $VERSION = '0'; + +Readonly::Scalar my $CONJUNCTION_OP => q[and]; +Readonly::Scalar my $DISJUNCTION_OP => q[or]; + +Readonly::Scalar my $ROBO_KEY => q[robo_qc]; +Readonly::Scalar my $LIBRARY_TYPE_KEY => q[library_type]; +Readonly::Scalar my $CRITERIA_KEY => q[acceptance_criteria]; + +## no critic (Documentation::RequirePodAtEnd) + +=head1 NAME + +npg_qc::autoqc::checks::meta + +=head1 SYNOPSIS + + my $check = npg_qc::autoqc::checks::meta->new(qc_in => 'dir_in'); + $check->execute(); + +=head1 DESCRIPTION + +This checks evaluates the results of other autoqc checks +against a pre-defined set of criteria. + +If data product acceptance criteria for a project and the +product's library type are defined, it is possible to introduce +a degree of automation into the manual QC process. To provide +interoperability with the API supporting the manual QC process, +the outcome of the evaluation performed by this check is recorded +not only as a simple pass or fail as in other autoqc checks, but +also as one of valid manual QC outcomes. A valid manual QC outcome +is one of the values from the library qc outcomes dictionary +(mqc_library_outcome_dict table of the npg_qc database), ie one +of 'Accepted', 'Rejected' or 'Undecided' outcomes. If the +final_qc_outcome flag of this class' instance is set to true, the +outcome is also marked as 'Final', otherwise it's marked as +'Preliminary' (examples: 'Accepted Final', 'Rejected Preliminary'). +By default the final_qc_outcome flag is false and the produced +outcomes are preliminary. + +We will not try to second-guess here what is and what is not +a product. Therefore, it is possible to invoke this check on +any entity apart from, possibly, a run. At run time an attempt +will be made to retrieve autoqc results due to be evaluated. +If this attempt fails, the execute method of the check will +exit with an error. A failure to retrieve the autoqc results +might be for one of three reasons: (1) either the entity is not +an end product (example: a pool) and no such results exist +or (2) it is a product, but the autoqc results have not been +computed yet, or (3) they have, but their file system location +(if that's where we are looking) is different from expected +(ie given by the qc_in attribute). + +The results of other autoqc checks are loaded either from the file +system (use_db attribute should be set to false, which is default) +or from a database (use_db attribute should be set to true). +npg_qc::autoqc::qc_store class is used to load results. In +contrast to npg_qc::autoqc::qc_store, if the database retrieval +is enabled, no fall back to a search on a file system is done. + +=head1 SUBROUTINES/METHODS + +=head2 use_db + +A boolean read-only attribute, false by default. +If set to false, autoqc results are loaded from the qc_in path. +If set to true, they are loaded from the database. + +=cut + +has 'use_db' => ( + isa => 'Bool', + is => 'ro', +); + +=head2 final_qc_outcome + +A boolean read-only attribute, false by default. +If set to false, the result of the evaluation is saved as a +preliminary manual QC outcome. If set to true, the result of the +evaluation is saved as a final manual QC outcome. + +=cut + +has 'final_qc_outcome' => ( + isa => 'Bool', + is => 'ro', +); + +=head2 conf_path + +An attribute, an absolute path of the directory with +the pipeline's configuration files. Inherited from +npg_tracking::util::pipeline_config + +=head2 conf_file_path + +A method. Return the path of the product configuration file. + Inherited from npg_tracking::util::pipeline_config + +=head2 can_run + +Returns true if the check can be run, ie relevant to the study +and library type evaluation criteria are defined, false otherwise. + +=cut + +sub can_run { + my $self = shift; + + if (not keys %{$self->_criteria}) { + $self->result->add_comment( + 'No criteria defined in the product configuration file'); + return 0; + } + return 1; +} + +=head2 execute + +Returns early if the can_run method returns false. Otherwise a full +evaluation of autoqc results for this product is performed. If auto +qc results necessary to perform the evaluation are not available, +an error is raised. + +=cut + +sub execute { + my $self = shift; + + $self->can_run() or return; + + $self->result->criteria($self->_criteria); + + return; +} + +has '_lims' => ( + isa => 'st::api::lims', + is => 'ro', + lazy_build => 1, +); +sub _build__lims { + my $self = shift; + return st::api::lims->new(rpt_list => $self->rpt_list); +} + +has '_criteria' => ( + isa => 'HashRef', + is => 'ro', + lazy_build => 1, +); +sub _build__criteria { + my $self = shift; + + my $rewritten = {}; + my @criteria = (); + + my $strict = 1; + my $study_config = $self->study_config($self->_lims(), $strict); + my $description = $self->composition->freeze; + + if (keys %{$study_config}) { + + if ($study_config->{$ROBO_KEY}) { + my $lib_type = $self->_lims->library_type; + $lib_type or croak "Library type is not defined for $description"; + $self->result->library_type($lib_type); + # We are going to compare library type strings in lower case + # because the case for this type of LIMs data might be inconsistent. + my $original_lib_type = $lib_type; + $lib_type = lc $lib_type; + + ##### + # We expect that the same criterium or a number of criteria can be + # relevant for multiple library types. Therefore, under the robo_qc + # section we expect a list of lists of criteria, each lower-level list + # being assiciated with at least one and potentially more library types. + # In practice under robo_qc we have a list of hashes, where each hash + # contains the 'library_type' key pointing to an array of relevant library + # types and the 'criteria' key pointing to an array or criteria. Criteria + # for a particular library type can be split between different hashes. + # + foreach my $criteria_set (@{$study_config->{$ROBO_KEY}}) { + $criteria_set->{$LIBRARY_TYPE_KEY} or croak "$LIBRARY_TYPE_KEY key is missing"; + $criteria_set->{$CRITERIA_KEY} or croak "$CRITERIA_KEY key is missing"; + if (any {$_ eq $lib_type} map { lc } @{$criteria_set->{$LIBRARY_TYPE_KEY}}) { + ##### + # A very simple criteria format - a list of strings - is used for now. + # Each string represents a math expression. It is assumed that the + # conjunction operator should be used to form the boolean expression + # that should give the result of the evaluation. Therefore, at the + # moment it is safe to collect all criteria in a single list. + # + push @criteria, @{$criteria_set->{$CRITERIA_KEY}}; + } + } + if (@criteria) { + # Applying the conjunction operator to all list members. + $rewritten = {$CONJUNCTION_OP => \@criteria}; + } else { + carp "No roboqc criteria defined for library type '$original_lib_type'"; + } + } else { + carp "$ROBO_KEY section is not present for $description"; + } + } else { + carp "Study config not found for $description"; + } + + return $rewritten; +} + +has '_expressions' => ( + isa => 'ArrayRef', + is => 'ro', + lazy_build => 1, +); +sub _build__expressions { + my $self = shift; + my $expressions = []; + _traverse($self->_criteria, $expressions); + return $expressions; +} + +has '_result_class_names' => ( + isa => 'ArrayRef', + is => 'ro', + lazy_build => 1, +); +sub _build__result_class_names { + my $self = shift; + + my @class_names = (); + foreach my $c ( @{$self->_expressions()} ) { + my ($class_name) = $c =~ /\A(?:\W+)?(\w+)[.]/xms; + $class_name or croak "Failed to infer class name from $c"; + push @class_names, $class_name; + } + @class_names = uniq sort @class_names; + return \@class_names; +} + +has '_qc_store' => ( + isa => 'npg_qc::autoqc::qc_store', + is => 'ro', + lazy_build => 1, +); +sub _build__qc_store { + my $self = shift; + return npg_qc::autoqc::qc_store->new( + use_db => $self->use_db, + checks_list => $self->_result_class_names + ); +} + +##### +# Two different approaches for loading results. We can try to perform +# evaluationing expression after expression and load the necessary result +# objects as we go. Or we can pre-load all results that will be needed. +# We choose the latter and raise an error if any results are missing. +# The error will report what is found and what is expected, so that +# the user has the full picture at once. +# +has '_results' => ( + isa => 'HashRef', + is => 'ro', + lazy_build => 1, +); +sub _build__results { + my $self = shift; + + my $collection = $self->use_db ? + $self->_qc_store->load_from_db4composition($self->composition) : + $self->_qc_store->load_from_path($self->qc_in); + + my $d = $self->composition->digest; + my @results = grep { $_->composition->digest eq $d } $collection->all; + + # We should have the right number of the right types of results. + my %h = map { $_ => 0 } @{$self->_result_class_names}; + foreach my $r (@results) { + my $class_name = $r->class_name; + exists $h{$class_name} or croak "Loaded unwanted class $class_name"; + ($h{$class_name} == 0) or croak "Multiple entries for class $class_name"; + $h{$class_name} = $r; + } + + my $num_found = scalar grep { $_ } values %h; + my $num_expected = scalar @{$self->_result_class_names}; + if ($num_expected != $num_found) { + my $m = join q[, ], @{$self->_result_class_names}; + $m = "Expected results for $m, found "; + $m .= $num_found + ? 'results for ' . join q[, ], grep { $h{$_} } sort keys %h + : 'none'; + croak $m; + } + + return \%h; +} + +sub _traverse { + my ($node, $expressions) = @_; + + # Simplest traversal for now. Ideally, this function should take + # a callback so that different actions can be performed. + my $node_type = ref $node; + if ($node_type) { + ($node_type eq 'HASH') or croak "Unknown node type $node_type"; + my @values = values %{$node}; + (scalar @values == 1) or croak 'More than one key-value pair'; + (ref $values[0] eq 'ARRAY') or croak 'Array value type expected'; + foreach my $n (@{$values[0]}) { + _traverse($n, $expressions); # recursion + } + } else { + push @{$expressions}, $node; # no need to recurse further + } + + return; +} + +__PACKAGE__->meta->make_immutable(); + +1; + +__END__ + +=head1 CONFIGURATION AND ENVIRONMENT + +=head1 DEPENDENCIES + +=over + +=item Moose + +=item namespace::autoclean + +=item Carp + +=item Readonly + +=item List::MoreUtils + +=item st::api::lims + +=item npg_tracking::util::pipeline_config + +=back + +=head1 INCOMPATIBILITIES + +=head1 BUGS AND LIMITATIONS + +=head1 AUTHOR + +Marina Gourtovaia + +=head1 LICENSE AND COPYRIGHT + +Copyright (C) 2019 GRL + +This file is part of NPG. + +NPG is free software: you can redistribute it and/or modify +it under the terms of the GNU General Public License as published by +the Free Software Foundation, either version 3 of the License, or +(at your option) any later version. + +This program is distributed in the hope that it will be useful, +but WITHOUT ANY WARRANTY; without even the implied warranty of +MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +GNU General Public License for more details. + +You should have received a copy of the GNU General Public License +along with this program. If not, see . + +=cut diff --git a/lib/npg_qc/autoqc/results/meta.pm b/lib/npg_qc/autoqc/results/meta.pm new file mode 100644 index 000000000..d8c0e5cf0 --- /dev/null +++ b/lib/npg_qc/autoqc/results/meta.pm @@ -0,0 +1,109 @@ +package npg_qc::autoqc::results::meta; + +use Moose; +use namespace::autoclean; +extends qw(npg_qc::autoqc::results::result); + +our $VERSION = '0'; + +## no critic (Documentation::RequirePodAtEnd) + +=head1 NAME + +npg_qc::autoqc::results::meta + +=head1 SYNOPSIS + +=head1 DESCRIPTION + +=head1 SUBROUTINES/METHODS + +=head2 library_type + +=cut + +has 'library_type' => ( + isa => 'Str', + is => 'rw', +); + +=head2 criteria + +A read-write hash reference attribute representing evaluation criteria +in a form that would not require any additional information to repeate +the evaluation as it was done at the time the check was run. + +All boolean operators are listed explicitly. The top-level expression +is either a conjunction or disjunction performed on a list of +expressions, each of wich can be, in turn, either a math expression or +a conjunction or disjunction. + +Examples: + + Assuming a = 2 and b = 5, + {'and' => ["a-1 < 0", "b+3 > 10"]} translates to + (a-1 > 0) && (b+3 > 10) and evaluates to false, while + {'or' => ["a-1 > 0", "b+3 > 10"]} translates to + (a-1 > 0) || (b+3 > 10) and evaluates to true. + + Assuming additionally c = 3 and d = 1, + {'and' => ["a-1 > 0", "b+3 > 5", {'or' => ["c-d > 0", "c-d < -1"]}]} + translates to + (a-1 > 0) && (b+3 > 5) && ((c-d > 0) || (c-d < -1)) and evaluates to true. + +=cut + +has 'criteria' => ( + isa => 'HashRef', + is => 'rw', + lazy_build => 1, +); + +__PACKAGE__->meta->make_immutable; + +1; + +__END__ + +=head1 DIAGNOSTICS + +=head1 CONFIGURATION AND ENVIRONMENT + +=head1 DEPENDENCIES + +=over + +=item Moose + +=item namespace::autoclean + +=back + +=head1 INCOMPATIBILITIES + +=head1 BUGS AND LIMITATIONS + +=head1 AUTHOR + +Marina Gourtovaia + +=head1 LICENSE AND COPYRIGHT + +Copyright (C) 2019 GRL + +This file is part of NPG. + +NPG is free software: you can redistribute it and/or modify +it under the terms of the GNU General Public License as published by +the Free Software Foundation, either version 3 of the License, or +(at your option) any later version. + +This program is distributed in the hope that it will be useful, +but WITHOUT ANY WARRANTY; without even the implied warranty of +MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +GNU General Public License for more details. + +You should have received a copy of the GNU General Public License +along with this program. If not, see . + +=cut diff --git a/t/60-autoqc-checks-meta.t b/t/60-autoqc-checks-meta.t new file mode 100644 index 000000000..8b9d12b55 --- /dev/null +++ b/t/60-autoqc-checks-meta.t @@ -0,0 +1,179 @@ +use strict; +use warnings; +use Test::More tests => 4; +use Test::Exception; +use Test::Warn; +use File::Temp qw/tempdir/; +use File::Copy; + +use_ok('npg_qc::autoqc::checks::meta'); + +my $dir = tempdir( CLEANUP => 1 ); +my $test_data_dir = 't/data/autoqc/meta'; +my $conf_file_path = "$test_data_dir/product_release.yml"; + +local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + 't/data/autoqc/meta/samplesheet_27483.csv'; + +subtest 'construction object, deciding whether to run' => sub { + plan tests => 18; + + my $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + isa_ok ($check, 'npg_qc::autoqc::checks::meta'); + isa_ok ($check->result, 'npg_qc::autoqc::results::meta'); + my $can_run; + warnings_like { $can_run = $check->can_run } + [qr/Reading product configuration from/, + qr/Study config not found for/], + 'can_run is accompanied by warnings'; + ok (!$can_run, 'can_run returns false - no study config'); + is ($check->result->comments, + 'No criteria defined in the product configuration file', + 'reason logged'); + lives_ok { $check->execute() } 'cannot run, but execute method runs OK'; + + $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/no_robo", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + warnings_like { $can_run = $check->can_run } + [qr/Reading product configuration from/, + qr/robo_qc section is not present for/], + 'can_run is accompanied by warnings'; + ok (!$can_run, 'can_run returns false - no robo config'); + is ($check->result->comments, + 'No criteria defined in the product configuration file', + 'reason logged'); + + $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/no_criteria", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + warnings_like { $can_run = $check->can_run } + [qr/Reading product configuration from/, + qr/No roboqc criteria defined for library type 'RNA PolyA'/], + 'can_run is accompanied by warnings'; + ok (!$can_run, 'can_run returns false - no criteria for this library type'); + is ($check->result->comments, + 'No criteria defined in the product configuration file', + 'reason logged'); + + $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/with_criteria", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + warnings_like { $can_run = $check->can_run } + [qr/Reading product configuration from/], + 'can_run is accompanied by warnings'; + ok ($can_run, 'can_run returns true'); + ok (!$check->result->comments, 'No comments logged'); + my $expected_criteria = {'and' => [ + '( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95', + 'bam_flagstats.target_mapped_bases > 85_000_000_000', + 'bam_flagstats.target_percent_gt_coverage_threshold > 95', + 'verify_bam_id.freemix < 0.01', + '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02' + ]}; + is_deeply ($check->_criteria, $expected_criteria, 'criteria parsed correctly'); + + $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/error1", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + throws_ok { $check->can_run } qr/acceptance_criteria key is missing/, + 'conf file format is incorrect'; + $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/error2", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + throws_ok { $check->can_run } qr/library_type key is missing/, + 'conf file format is incorrect'; +}; + +subtest 'setting options for qc store' => sub { + plan tests => 4; + + my $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/with_criteria", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + my $expected_class_names = [qw/bam_flagstats bcfstats verify_bam_id/]; + is_deeply ($check->_result_class_names, $expected_class_names, 'class names'); + is_deeply ($check->_qc_store->checks_list, $expected_class_names, + 'class names correctly propagated to the qc store object'); + + ok (!$check->use_db, 'default is not to use the db'); + ok (!$check->_qc_store->use_db, + 'db option correctly propagated to the qc store object'); +}; + +subtest 'finding files - file system' => sub { + plan tests => 15; + + my $expected = 'Expected results for bam_flagstats, bcfstats, verify_bam_id,'; + + my $check = npg_qc::autoqc::checks::meta->new( + conf_path => "$test_data_dir/with_criteria", + qc_in => $test_data_dir, + rpt_list => '27483:1:2'); + throws_ok { $check->_results } qr/$expected found none/, 'no results - error'; + + # should have all three for the entiry and phix for bam_flagstats and qx_yield + # and gradually add them to qc_in + + local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + 't/data/autoqc/meta/samplesheet_29524.csv'; + + for my $name (('29524#2.qX_yield.json', + '29524#2_phix.bam_flagstats.json', + '29524#7.bam_flagstats.json')) { + copy "$test_data_dir/$name", "$dir/$name"; + my $c = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $dir, + rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + throws_ok { $c->_results } qr/$expected found none/, 'no results - error'; + } + + my $name = '29524#2.bam_flagstats.json'; + copy "$test_data_dir/$name", "$dir/$name"; + $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $dir, + rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + throws_ok { $check->_results } + qr/$expected found results for bam_flagstats/, 'not all results - error'; + + $name = '29524#2.verify_bam_id.json'; + copy "$test_data_dir/$name", "$dir/$name"; + $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $dir, + rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + throws_ok { $check->_results } + qr/$expected found results for bam_flagstats, verify_bam_id/, + 'not all results - error'; + + $name = '29524#2.bcfstats.json'; + copy "$test_data_dir/$name", "$dir/$name"; + $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $test_data_dir, + rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + lives_ok { $check->_results } 'no error - all expected results loaded'; + is_deeply ([sort keys %{$check->_results}], + [sort qw/bam_flagstats bcfstats verify_bam_id/], 'correct keys'); + + for my $name (keys %{$check->_results}) { + my $result = $check->_results->{$name}; + is (ref $result, 'npg_qc::autoqc::results::'. $name, 'corrrect type of object hashed'); + is ($result->composition->get_component(0)->tag_index, 2, 'tag index correct'); + } + ok (!$check->_results->{bam_flagstats}->composition->get_component(0)->subset, + 'subset is undefined despite phix result present in test data'); +}; + +1; diff --git a/t/60-autoqc-results-meta.t b/t/60-autoqc-results-meta.t new file mode 100644 index 000000000..e7b393e80 --- /dev/null +++ b/t/60-autoqc-results-meta.t @@ -0,0 +1,8 @@ +use strict; +use warnings; +use Test::More tests => 1; +use Test::Exception; + +use_ok('npg_qc::autoqc::results::meta'); + +1; diff --git a/t/data/autoqc/meta/29524#17.bam_flagstats.json b/t/data/autoqc/meta/29524#17.bam_flagstats.json new file mode 100644 index 000000000..75c8f553a --- /dev/null +++ b/t/data/autoqc/meta/29524#17.bam_flagstats.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::bam_flagstats-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":17},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":17},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":17},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":17}]},"histogram":{"1":"1.01565","10":"4.10006","100":"4.42679","11":"4.17502","12":"4.23279","13":"4.2773","14":"4.3116","15":"4.33803","16":"4.35839","17":"4.37409","18":"4.38618","19":"4.3955","2":"1.79828","20":"4.40268","21":"4.40821","22":"4.41247","23":"4.41576","24":"4.41829","25":"4.42024","26":"4.42175","27":"4.4229","28":"4.4238","29":"4.42448","3":"2.40134","30":"4.42501","31":"4.42542","32":"4.42574","33":"4.42598","34":"4.42617","35":"4.42631","36":"4.42642","37":"4.42651","38":"4.42657","39":"4.42662","4":"2.86605","40":"4.42666","41":"4.42669","42":"4.42672","43":"4.42673","44":"4.42675","45":"4.42676","46":"4.42677","47":"4.42677","48":"4.42678","49":"4.42678","5":"3.22413","50":"4.42678","51":"4.42679","52":"4.42679","53":"4.42679","54":"4.42679","55":"4.42679","56":"4.42679","57":"4.42679","58":"4.42679","59":"4.42679","6":"3.50006","60":"4.42679","61":"4.42679","62":"4.42679","63":"4.42679","64":"4.42679","65":"4.42679","66":"4.42679","67":"4.42679","68":"4.42679","69":"4.42679","7":"3.71269","70":"4.42679","71":"4.42679","72":"4.42679","73":"4.42679","74":"4.42679","75":"4.42679","76":"4.42679","77":"4.42679","78":"4.42679","79":"4.42679","8":"3.87652","80":"4.42679","81":"4.42679","82":"4.42679","83":"4.42679","84":"4.42679","85":"4.42679","86":"4.42679","87":"4.42679","88":"4.42679","89":"4.42679","9":"4.00277","90":"4.42679","91":"4.42679","92":"4.42679","93":"4.42679","94":"4.42679","95":"4.42679","96":"4.42679","97":"4.42679","98":"4.42679","99":"4.42679"},"info":{"Check":"npg_qc::autoqc::checks::bam_flagstats","Check_version":"66.4","markdups_metrics_header":"# /software/pkg/biobambam2/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex17/bmdfopt_29524#17.tmp M=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex17/29524#17.markdups_metrics.txt\n"},"library":"25385024","library_size":1287367589,"mate_mapped_defferent_chr":5077986,"mate_mapped_defferent_chr_5":2493403,"num_total_reads":674587374,"paired_mapped_reads":335608716,"paired_read_duplicates":44711252,"path":"/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex17","percent_duplicate":0.133696,"proper_mapped_pair":663014288,"read_pair_optical_duplicates":5892783,"read_pairs_examined":335523856,"target_coverage_threshold":15,"target_filter":"F0xF04_target","target_length":2945869055,"target_mapped_bases":86462915017,"target_mapped_reads":577942011,"target_percent_gt_coverage_threshold":94.17,"target_proper_pair_mapped_reads":570627398,"unmapped_reads":1814724,"unpaired_mapped_reads":1555218,"unpaired_read_duplicates":501560} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#2.bam_flagstats.json b/t/data/autoqc/meta/29524#2.bam_flagstats.json new file mode 100644 index 000000000..3157c47db --- /dev/null +++ b/t/data/autoqc/meta/29524#2.bam_flagstats.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::bam_flagstats-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":2}]},"histogram":{"1":"1.01552","10":"4.09378","100":"4.41808","11":"4.16832","12":"4.22573","13":"4.26994","14":"4.30399","15":"4.33022","16":"4.35041","17":"4.36597","18":"4.37795","19":"4.38717","2":"1.79762","20":"4.39428","21":"4.39975","22":"4.40396","23":"4.40721","24":"4.40971","25":"4.41163","26":"4.41311","27":"4.41426","28":"4.41513","29":"4.41581","3":"2.39995","30":"4.41633","31":"4.41673","32":"4.41704","33":"4.41728","34":"4.41747","35":"4.41761","36":"4.41772","37":"4.4178","38":"4.41786","39":"4.41791","4":"2.86383","40":"4.41795","41":"4.41798","42":"4.418","43":"4.41802","44":"4.41804","45":"4.41805","46":"4.41805","47":"4.41806","48":"4.41806","49":"4.41807","5":"3.22108","50":"4.41807","51":"4.41807","52":"4.41808","53":"4.41808","54":"4.41808","55":"4.41808","56":"4.41808","57":"4.41808","58":"4.41808","59":"4.41808","6":"3.49622","60":"4.41808","61":"4.41808","62":"4.41808","63":"4.41808","64":"4.41808","65":"4.41808","66":"4.41808","67":"4.41808","68":"4.41808","69":"4.41808","7":"3.70812","70":"4.41808","71":"4.41808","72":"4.41808","73":"4.41808","74":"4.41808","75":"4.41808","76":"4.41808","77":"4.41808","78":"4.41808","79":"4.41808","8":"3.87131","80":"4.41808","81":"4.41808","82":"4.41808","83":"4.41808","84":"4.41808","85":"4.41808","86":"4.41808","87":"4.41808","88":"4.41808","89":"4.41808","9":"3.99698","90":"4.41808","91":"4.41808","92":"4.41808","93":"4.41808","94":"4.41808","95":"4.41808","96":"4.41808","97":"4.41808","98":"4.41808","99":"4.41808"},"info":{"Check":"npg_qc::autoqc::checks::bam_flagstats","Check_version":"66.4","markdups_metrics_header":"# /software/pkg/biobambam2/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2/bmdfopt_29524#2.tmp M=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2/29524#2.markdups_metrics.txt\n"},"library":"25385034","library_size":1284294818,"mate_mapped_defferent_chr":5134388,"mate_mapped_defferent_chr_5":2604768,"num_total_reads":674316890,"paired_mapped_reads":335514087,"paired_read_duplicates":44738596,"path":"/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2","percent_duplicate":0.133789,"proper_mapped_pair":662661026,"read_pair_optical_duplicates":5845593,"read_pairs_examined":335429261,"target_coverage_threshold":15,"target_filter":"F0xF04_target","target_length":2945869055,"target_mapped_bases":86482129273,"target_mapped_reads":577690137,"target_percent_gt_coverage_threshold":96.81,"target_proper_pair_mapped_reads":570108400,"unmapped_reads":1771277,"unpaired_mapped_reads":1517439,"unpaired_read_duplicates":479118} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#2.bcfstats.json b/t/data/autoqc/meta/29524#2.bcfstats.json new file mode 100644 index 000000000..ebcc2e5f5 --- /dev/null +++ b/t/data/autoqc/meta/29524#2.bcfstats.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::bcfstats-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":2}]},"expected_sample_name":"5455485","geno_refset_name":"study5392","geno_refset_path":"/lustre/scratch113/npg_repository/geno_refset/study5392/GRCh38_15_plus_hs38d1/bcftools/study5392.annotation.vcf","genotypes_attempted":782541,"genotypes_called":781684,"genotypes_compared":205058,"genotypes_concordant":204573,"genotypes_nrd_dividend":485,"genotypes_nrd_divisor":199979,"genotypes_passed":779921,"info":{"Caller":"/software/pkg/bcftools/1.8/bin/bcftools","Calling_command":"/software/pkg/bcftools/1.8/bin/bcftools mpileup --min-BQ 20 --annotate 'FORMAT/AD,FORMAT/DP' --max-depth 50000 --targets-file /lustre/scratch113/npg_repository/geno_refset/study5392/GRCh38_15_plus_hs38d1/bcftools/study5392.annotation.vcf --fasta-ref /lustre/scratch113/npg_repository/references/Homo_sapiens/GRCh38_15_plus_hs38d1/all/fasta/Homo_sapiens.GRCh38_15_plus_hs38d1.fa --output-type u /lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2/29524#2.cram | /software/pkg/bcftools/1.8/bin/bcftools call --multiallelic-caller --keep-alts --skip-variants indels --ploidy-file /lustre/scratch113/npg_repository/geno_refset/study5392/GRCh38_15_plus_hs38d1/bcftools/study5392.ploidy --output-type u | /software/pkg/bcftools/1.8/bin/bcftools filter --mode + --soft-filter LowDepth --exclude 'FORMAT/DP<8' --output-type v | /software/pkg/bcftools/1.8/bin/bcftools filter --mode + --soft-filter LowQual --exclude '%QUAL<20 || MQ<20 || GT=\"RR\" && FORMAT/AD[0:0]/FORMAT/DP < 0.8 || GT=\"AA\" && FORMAT/AD[0:0]/FORMAT/DP > 0.2 || GT=\"RA\" && FORMAT/AD[0:0]/FORMAT/DP < 0.2 || GT=\"RA\" && FORMAT/AD[0:0]/FORMAT/DP > 0.8' --output-type v ","Check":"npg_qc::autoqc::checks::bcfstats","Check_version":"66.4","Criterion":"NRD % < 2"},"pass":"1"} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#2.qX_yield.json b/t/data/autoqc/meta/29524#2.qX_yield.json new file mode 100644 index 000000000..d580bac28 --- /dev/null +++ b/t/data/autoqc/meta/29524#2.qX_yield.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::qX_yield-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":2}]},"filename1":"29524#2_F0xB00.stats","filename2":"29524#2_F0xB00.stats","info":{"Check":"npg_qc::autoqc::checks::qX_yield","Check_version":"66.4"},"path":"/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2","threshold_quality":20,"yield1":50020873,"yield1_q30":48381583,"yield1_q40":0,"yield2":49552731,"yield2_q30":47290289,"yield2_q40":0} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#2.verify_bam_id.json b/t/data/autoqc/meta/29524#2.verify_bam_id.json new file mode 100644 index 000000000..3f9478eeb --- /dev/null +++ b/t/data/autoqc/meta/29524#2.verify_bam_id.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::verify_bam_id-66.4","avg_depth":28.72,"composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":2}]},"freeLK0":8977857.45,"freeLK1":8977078.11,"freemix":0.00026,"info":{"Check":"npg_qc::autoqc::checks::verify_bam_id","Check_version":"66.4","Criterion":"snps > 10000, average depth >= 2 and freemix < 0.05","Verifier":"verifyBamID","Verify_options":" --bam /lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2/29524#2.bam --vcf /lustre/scratch113/npg_repository/population_snv/Homo_sapiens/1000G_Omni25_genotypes_2141/Standard/GRCh38_15_plus_hs38d1/Standard-GRCh38_15_plus_hs38d1.vcf.gz --self --ignoreRG --minQ 20 --minAF 0.05 --maxDepth 500 --precise --out /tmp/wr_cwd/d/b/b/c56fdffa375c26f89c26b7a5ef4bb236887305/tmp/n0rGXI3kaP/29524#2.bam"},"number_of_reads":37696039,"number_of_snps":1312402,"pass":"1"} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#2_phix.bam_flagstats.json b/t/data/autoqc/meta/29524#2_phix.bam_flagstats.json new file mode 100644 index 000000000..a4ae9e644 --- /dev/null +++ b/t/data/autoqc/meta/29524#2_phix.bam_flagstats.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::bam_flagstats-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"subset":"phix","tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"subset":"phix","tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"subset":"phix","tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"subset":"phix","tag_index":2}]},"histogram":{},"info":{"Check":"npg_qc::autoqc::checks::bam_flagstats","Check_version":"66.4","markdups_metrics_header":"# /software/pkg/biobambam2/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2/bmdfopp_29524#2_phix.tmp M=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2/29524#2_phix.markdups_metrics.txt\n"},"library":"25385034","library_size":null,"mate_mapped_defferent_chr":0,"mate_mapped_defferent_chr_5":0,"num_total_reads":2,"paired_mapped_reads":0,"paired_read_duplicates":0,"path":"/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex2","percent_duplicate":0,"proper_mapped_pair":0,"read_pair_optical_duplicates":0,"read_pairs_examined":0,"subset":"phix","unmapped_reads":1,"unpaired_mapped_reads":1,"unpaired_read_duplicates":0} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#3.bam_flagstats.json b/t/data/autoqc/meta/29524#3.bam_flagstats.json new file mode 100644 index 000000000..307a84aba --- /dev/null +++ b/t/data/autoqc/meta/29524#3.bam_flagstats.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::bam_flagstats-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":3},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":3},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":3},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":3}]},"histogram":{"1":"1.01578","10":"4.10989","100":"4.44055","11":"4.18552","12":"4.24386","13":"4.28885","14":"4.32355","15":"4.35032","16":"4.37096","17":"4.38687","18":"4.39915","19":"4.40862","2":"1.7992","20":"4.41592","21":"4.42156","22":"4.4259","23":"4.42925","24":"4.43183","25":"4.43383","26":"4.43536","27":"4.43655","28":"4.43746","29":"4.43817","3":"2.40341","30":"4.43871","31":"4.43913","32":"4.43946","33":"4.4397","34":"4.4399","35":"4.44005","36":"4.44016","37":"4.44025","38":"4.44032","39":"4.44037","4":"2.86941","40":"4.44041","41":"4.44044","42":"4.44046","43":"4.44048","44":"4.4405","45":"4.44051","46":"4.44052","47":"4.44052","48":"4.44053","49":"4.44053","5":"3.22881","50":"4.44054","51":"4.44054","52":"4.44054","53":"4.44054","54":"4.44054","55":"4.44054","56":"4.44054","57":"4.44054","58":"4.44054","59":"4.44054","6":"3.50599","60":"4.44055","61":"4.44055","62":"4.44055","63":"4.44055","64":"4.44055","65":"4.44055","66":"4.44055","67":"4.44055","68":"4.44055","69":"4.44055","7":"3.71977","70":"4.44055","71":"4.44055","72":"4.44055","73":"4.44055","74":"4.44055","75":"4.44055","76":"4.44055","77":"4.44055","78":"4.44055","79":"4.44055","8":"3.88465","80":"4.44055","81":"4.44055","82":"4.44055","83":"4.44055","84":"4.44055","85":"4.44055","86":"4.44055","87":"4.44055","88":"4.44055","89":"4.44055","9":"4.01181","90":"4.44055","91":"4.44055","92":"4.44055","93":"4.44055","94":"4.44055","95":"4.44055","96":"4.44055","97":"4.44055","98":"4.44055","99":"4.44055"},"info":{"Check":"npg_qc::autoqc::checks::bam_flagstats","Check_version":"66.4","markdups_metrics_header":"# /software/pkg/biobambam2/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex3/bmdfopt_29524#3.tmp M=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex3/29524#3.markdups_metrics.txt\n"},"library":"25385046","library_size":1160227821,"mate_mapped_defferent_chr":4462326,"mate_mapped_defferent_chr_5":2240410,"num_total_reads":605808358,"paired_mapped_reads":301438864,"paired_read_duplicates":40082034,"path":"/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex3","percent_duplicate":0.1334,"proper_mapped_pair":595351432,"read_pair_optical_duplicates":5333639,"read_pairs_examined":301362479,"target_coverage_threshold":15,"target_filter":"F0xF04_target","target_length":2945869055,"target_mapped_bases":77713281072,"target_mapped_reads":519121523,"target_percent_gt_coverage_threshold":95.22,"target_proper_pair_mapped_reads":512365188,"unmapped_reads":1564138,"unpaired_mapped_reads":1366492,"unpaired_read_duplicates":421691} \ No newline at end of file diff --git a/t/data/autoqc/meta/29524#7.bam_flagstats.json b/t/data/autoqc/meta/29524#7.bam_flagstats.json new file mode 100644 index 000000000..091684f0a --- /dev/null +++ b/t/data/autoqc/meta/29524#7.bam_flagstats.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_qc::autoqc::results::bam_flagstats-66.4","composition":{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":7},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":7},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":7},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":7}]},"histogram":{"1":"1.01624","10":"3.95975","100":"4.2311","11":"4.02492","12":"4.07444","13":"4.11207","14":"4.14066","15":"4.16238","16":"4.17889","17":"4.19143","18":"4.20096","19":"4.2082","2":"1.7884","20":"4.2137","21":"4.21788","22":"4.22106","23":"4.22347","24":"4.2253","25":"4.2267","26":"4.22776","27":"4.22856","28":"4.22917","29":"4.22964","3":"2.37509","30":"4.22999","31":"4.23026","32":"4.23046","33":"4.23062","34":"4.23073","35":"4.23082","36":"4.23089","37":"4.23094","38":"4.23098","39":"4.23101","4":"2.82088","40":"4.23103","41":"4.23105","42":"4.23106","43":"4.23107","44":"4.23108","45":"4.23109","46":"4.23109","47":"4.23109","48":"4.2311","49":"4.2311","5":"3.15959","50":"4.2311","51":"4.2311","52":"4.2311","53":"4.2311","54":"4.2311","55":"4.2311","56":"4.2311","57":"4.2311","58":"4.2311","59":"4.2311","6":"3.41695","60":"4.2311","61":"4.2311","62":"4.2311","63":"4.2311","64":"4.2311","65":"4.2311","66":"4.2311","67":"4.2311","68":"4.2311","69":"4.2311","7":"3.6125","70":"4.2311","71":"4.2311","72":"4.2311","73":"4.2311","74":"4.2311","75":"4.2311","76":"4.2311","77":"4.2311","78":"4.2311","79":"4.2311","8":"3.76108","80":"4.2311","81":"4.2311","82":"4.2311","83":"4.2311","84":"4.2311","85":"4.2311","86":"4.2311","87":"4.2311","88":"4.2311","89":"4.2311","9":"3.87397","90":"4.2311","91":"4.2311","92":"4.2311","93":"4.2311","94":"4.2311","95":"4.2311","96":"4.2311","97":"4.2311","98":"4.2311","99":"4.2311"},"info":{"Check":"npg_qc::autoqc::checks::bam_flagstats","Check_version":"66.4","markdups_metrics_header":"# /software/pkg/biobambam2/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex7/bmdfopt_29524#7.tmp M=/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex7/29524#7.markdups_metrics.txt\n"},"library":"25385094","library_size":1198927185,"mate_mapped_defferent_chr":5029328,"mate_mapped_defferent_chr_5":2435003,"num_total_reads":662064832,"paired_mapped_reads":329402410,"paired_read_duplicates":45958389,"path":"/lustre/scratch113/esa-sv-20190409-01/IL_seq_data/analysis/190517_A00538_0110_AHJL2YDSXX/Data/Intensities/BAM_basecalls_20190519-074210/no_cal/archive/plex7","percent_duplicate":0.140001,"proper_mapped_pair":650117330,"read_pair_optical_duplicates":6041152,"read_pairs_examined":329318707,"target_coverage_threshold":15,"target_filter":"F0xF04_target","target_length":2945869055,"target_mapped_bases":83930772951,"target_mapped_reads":560632991,"target_percent_gt_coverage_threshold":93.67,"target_proper_pair_mapped_reads":553614308,"unmapped_reads":1746955,"unpaired_mapped_reads":1513057,"unpaired_read_duplicates":505043} \ No newline at end of file diff --git a/t/data/autoqc/meta/error1/product_release.yml b/t/data/autoqc/meta/error1/product_release.yml new file mode 100644 index 000000000..a4a17dce9 --- /dev/null +++ b/t/data/autoqc/meta/error1/product_release.yml @@ -0,0 +1,37 @@ +--- +default: + s3: + enable: false + url: null + notify: false + irods: + enable: true + notify: false + +study: + - study_id: "4042" + s3: + enable: true + url: "gs://profile_one-europe-west2" + date_binning: true + customer_name: "UK Organisation" + profile: "profile_one" + notify: true + irods: + enable: false + notify: true + merge: + component_cache_dir: "/merge_component_cache/4042/" + robo_qc: + - library_type: + - "RNA PolyA" + criteria : + - "( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95" + - "bam_flagstats.target_mapped_bases > 85_000_000_000" + - "bam_flagstats.target_percent_gt_coverage_threshold > 95" + - library_type: + - "HiSeqX PCR free" + - "rna PolyA" + acceptance_criteria : + - "verify_bam_id.freemix < 0.01" + - "( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02" diff --git a/t/data/autoqc/meta/error2/product_release.yml b/t/data/autoqc/meta/error2/product_release.yml new file mode 100644 index 000000000..3aad22ceb --- /dev/null +++ b/t/data/autoqc/meta/error2/product_release.yml @@ -0,0 +1,37 @@ +--- +default: + s3: + enable: false + url: null + notify: false + irods: + enable: true + notify: false + +study: + - study_id: "4042" + s3: + enable: true + url: "gs://profile_one-europe-west2" + date_binning: true + customer_name: "UK Organisation" + profile: "profile_one" + notify: true + irods: + enable: false + notify: true + merge: + component_cache_dir: "/merge_component_cache/4042/" + robo_qc: + - library_type: + - "RNA PolyA" + acceptance_criteria : + - "( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95" + - "bam_flagstats.target_mapped_bases > 85_000_000_000" + - "bam_flagstats.target_percent_gt_coverage_threshold > 95" + - type: + - "HiSeqX PCR free" + - "rna PolyA" + acceptance_criteria : + - "verify_bam_id.freemix < 0.01" + - "( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02" diff --git a/t/data/autoqc/meta/no_criteria/product_release.yml b/t/data/autoqc/meta/no_criteria/product_release.yml new file mode 100644 index 000000000..b55f74be7 --- /dev/null +++ b/t/data/autoqc/meta/no_criteria/product_release.yml @@ -0,0 +1,36 @@ +--- +default: + s3: + enable: false + url: null + notify: false + irods: + enable: true + notify: false + +study: + - study_id: "4042" + s3: + enable: true + url: "gs://profile_one-europe-west2" + date_binning: true + customer_name: "UK Organisation" + profile: "profile_one" + notify: true + irods: + enable: false + notify: true + merge: + component_cache_dir: "/merge_component_cache/4042/" + robo_qc: + - library_type: + - "HiSeqX PCR free" + acceptance_criteria : + - "( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95" + - "bam_flagstats.target_mapped_bases > 85_000_000_000" + - "bam_flagstats.target_percent_gt_coverage_threshold > 95" + - library_type: + - "HiSeqX PCR free" + - "Bisulphite pre quality controlled" + acceptance_criteria : + - "verify_bam_id.freemix < 0.01" diff --git a/t/data/autoqc/meta/no_robo/product_release.yml b/t/data/autoqc/meta/no_robo/product_release.yml new file mode 100644 index 000000000..a9084bf1e --- /dev/null +++ b/t/data/autoqc/meta/no_robo/product_release.yml @@ -0,0 +1,24 @@ +--- +default: + s3: + enable: false + url: null + notify: false + irods: + enable: true + notify: false + +study: + - study_id: "4042" + s3: + enable: true + url: "gs://profile_one-europe-west2" + date_binning: true + customer_name: "UK Organisation" + profile: "profile_one" + notify: true + irods: + enable: false + notify: true + merge: + component_cache_dir: "merge_component_cache/4042/" diff --git a/t/data/autoqc/meta/product_release.yml b/t/data/autoqc/meta/product_release.yml new file mode 100644 index 000000000..859fc1539 --- /dev/null +++ b/t/data/autoqc/meta/product_release.yml @@ -0,0 +1,34 @@ +--- +default: + s3: + enable: false + url: null + notify: false + irods: + enable: true + notify: false + +study: + - study_id: "5392" + s3: + enable: true + url: "gs://profile_one-europe-west2" + date_binning: true + customer_name: "UK Organisation" + profile: "profile_one" + notify: true + receipts: "/data_product_receipts/5392/" + irods: + enable: false + notify: true + merge: + component_cache_dir: "/merge_component_cache/5392/" + robo_qc: + - library_type: + - "HiSeqX PCR free" + acceptance_criteria : + - "( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95" + - "bam_flagstats.target_mapped_bases > 85_000_000_000" + - "bam_flagstats.target_percent_gt_coverage_threshold > 95" + - "verify_bam_id.freemix < 0.01" + - "( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02" diff --git a/t/data/autoqc/meta/samplesheet_27483.csv b/t/data/autoqc/meta/samplesheet_27483.csv new file mode 120000 index 000000000..a8542fb66 --- /dev/null +++ b/t/data/autoqc/meta/samplesheet_27483.csv @@ -0,0 +1 @@ +../../../../t/data/autoqc/verify_bam_id/samplesheet_27483.csv \ No newline at end of file diff --git a/t/data/autoqc/meta/with_criteria/product_release.yml b/t/data/autoqc/meta/with_criteria/product_release.yml new file mode 100644 index 000000000..65ffb2cbc --- /dev/null +++ b/t/data/autoqc/meta/with_criteria/product_release.yml @@ -0,0 +1,37 @@ +--- +default: + s3: + enable: false + url: null + notify: false + irods: + enable: true + notify: false + +study: + - study_id: "4042" + s3: + enable: true + url: "gs://profile_one-europe-west2" + date_binning: true + customer_name: "UK Organisation" + profile: "profile_one" + notify: true + irods: + enable: false + notify: true + merge: + component_cache_dir: "/merge_component_cache/4042/" + robo_qc: + - library_type: + - "RNA PolyA" + acceptance_criteria : + - "( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95" + - "bam_flagstats.target_mapped_bases > 85_000_000_000" + - "bam_flagstats.target_percent_gt_coverage_threshold > 95" + - library_type: + - "HiSeqX PCR free" + - "rna PolyA" + acceptance_criteria : + - "verify_bam_id.freemix < 0.01" + - "( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02" From acca8ecf51352d9aa56d2b172c1ba51eb37efc00 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Thu, 23 May 2019 18:12:53 +0100 Subject: [PATCH 08/23] loading input result objects from a database --- MANIFEST | 1 + lib/npg_qc/autoqc/checks/meta.pm | 2 +- lib/npg_qc/autoqc/qc_store.pm | 53 ++++++++++++ t/60-autoqc-checks-meta.t | 90 +++++++++++++++++++-- t/data/autoqc/meta/29524#2.composition.json | 1 + 5 files changed, 141 insertions(+), 6 deletions(-) create mode 100644 t/data/autoqc/meta/29524#2.composition.json diff --git a/MANIFEST b/MANIFEST index abd4fdc37..06b2589ec 100644 --- a/MANIFEST +++ b/MANIFEST @@ -424,6 +424,7 @@ t/data/autoqc/load/do_not_add_files_to_this_dir t/data/autoqc/load/some.json t/data/autoqc/meta/product_release.yml t/data/autoqc/meta/samplesheet_27483.csv +t/data/autoqc/meta/29524#2.composition.json t/data/autoqc/meta/29524#17.bam_flagstats.json t/data/autoqc/meta/29524#2.bam_flagstats.json t/data/autoqc/meta/29524#2.bcfstats.json diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/meta.pm index 1edf0df6f..9fba978bb 100644 --- a/lib/npg_qc/autoqc/checks/meta.pm +++ b/lib/npg_qc/autoqc/checks/meta.pm @@ -286,7 +286,7 @@ sub _build__results { my $self = shift; my $collection = $self->use_db ? - $self->_qc_store->load_from_db4composition($self->composition) : + $self->_qc_store->load_from_db_via_composition([$self->composition]) : $self->_qc_store->load_from_path($self->qc_in); my $d = $self->composition->digest; diff --git a/lib/npg_qc/autoqc/qc_store.pm b/lib/npg_qc/autoqc/qc_store.pm index ec774257b..c16fb0385 100644 --- a/lib/npg_qc/autoqc/qc_store.pm +++ b/lib/npg_qc/autoqc/qc_store.pm @@ -372,6 +372,43 @@ sub load_from_db { return $c; } +=head2 load_from_db_via_composition + +Similar to load_from_db, but loads database results for an array of composition +objects. + +Returns a collection object (npg_qc::autoqc::results::collection) containing +autoqc result corresponding to the argument compositions of types listed +in the checks_list attribute. Returns an empty collection if no results are +found. + + # Assuming $c1 and $c2 are objects of type npg_tracking::glosary::composition + my $c = $obj->load_from_db_via_composition([$c1, $c2]); + +=cut + +sub load_from_db_via_composition { + my ($self, $compositions) = @_; + + $compositions or croak + 'Array of composition objects should be given'; + + my @results = (); + if ($self->use_db && @{$compositions}) { + my $rs = $self->qc_schema()->resultset('SeqComposition')->search( + {'me.digest' => [map { $_->digest } @{$compositions}]}, + {join => $self->_relation_names} + ); + while (my $row = $rs->next()) { + push @results, grep { $_ } map { $row->$_ } @{$self->_relation_names}; + } + } + my $c = npg_qc::autoqc::results::collection->new(); + $c->add(\@results); + + return $c; +} + =head2 json_file2result_object Reads an argument JSON file and converts the content into in-memory autoqc @@ -414,6 +451,22 @@ sub json_file2result_object { return $result; } +has '_relation_names' => ( + isa => 'ArrayRef', + is => 'ro', + required => 0, + lazy_build => 1, +); +sub _build__relation_names { + my $self = shift; + my @names = (); + foreach my $n (@{$self->checks_list()}) { + $n =~ s/s\Z//xms; + push @names, $n; + } + return \@names; +} + sub _runfolder_obj { my ($self, $query) = @_; diff --git a/t/60-autoqc-checks-meta.t b/t/60-autoqc-checks-meta.t index 8b9d12b55..1d6e617dc 100644 --- a/t/60-autoqc-checks-meta.t +++ b/t/60-autoqc-checks-meta.t @@ -1,10 +1,16 @@ use strict; use warnings; -use Test::More tests => 4; +use Test::More tests => 5; use Test::Exception; use Test::Warn; use File::Temp qw/tempdir/; use File::Copy; +use Perl6::Slurp; +use JSON qw/from_json/; + +use npg_testing::db; +use npg_qc::autoqc::qc_store; +use npg_tracking::glossary::composition; use_ok('npg_qc::autoqc::checks::meta'); @@ -110,10 +116,11 @@ subtest 'setting options for qc store' => sub { 'db option correctly propagated to the qc store object'); }; -subtest 'finding files - file system' => sub { +subtest 'finding result - file system' => sub { plan tests => 15; my $expected = 'Expected results for bam_flagstats, bcfstats, verify_bam_id,'; + my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; my $check = npg_qc::autoqc::checks::meta->new( conf_path => "$test_data_dir/with_criteria", @@ -134,7 +141,7 @@ subtest 'finding files - file system' => sub { my $c = npg_qc::autoqc::checks::meta->new( conf_path => $test_data_dir, qc_in => $dir, - rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + rpt_list => $rpt_list); throws_ok { $c->_results } qr/$expected found none/, 'no results - error'; } @@ -143,7 +150,7 @@ subtest 'finding files - file system' => sub { $check = npg_qc::autoqc::checks::meta->new( conf_path => $test_data_dir, qc_in => $dir, - rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + rpt_list => $rpt_list); throws_ok { $check->_results } qr/$expected found results for bam_flagstats/, 'not all results - error'; @@ -162,7 +169,7 @@ subtest 'finding files - file system' => sub { $check = npg_qc::autoqc::checks::meta->new( conf_path => $test_data_dir, qc_in => $test_data_dir, - rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); + rpt_list => $rpt_list); lives_ok { $check->_results } 'no error - all expected results loaded'; is_deeply ([sort keys %{$check->_results}], [sort qw/bam_flagstats bcfstats verify_bam_id/], 'correct keys'); @@ -176,4 +183,77 @@ subtest 'finding files - file system' => sub { 'subset is undefined despite phix result present in test data'); }; +subtest 'finding results - database' => sub { + plan tests => 15; + + local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + 't/data/autoqc/meta/samplesheet_29524.csv'; + + my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; + + my $schema = Moose::Meta::Class->create_anon_class( + roles => [qw/npg_testing::db/])->new_object() + ->create_test_db(q[npg_qc::Schema]); + my $init = { + conf_path => $test_data_dir, + rpt_list => $rpt_list, + use_db => 1, + _qc_store => npg_qc::autoqc::qc_store->new( + use_db => 1, + qc_schema => $schema, + checks_list => [qw/bam_flagstats bcfstats verify_bam_id/] + ) + }; + my $check; + lives_ok { $check = npg_qc::autoqc::checks::meta->new($init) } + 'object created without qc_in defined'; + + my $expected = 'Expected results for bam_flagstats, bcfstats, verify_bam_id,'; + throws_ok { $check->_results } qr/$expected found none/, 'no results - error'; + + # create composition, but not records in the result tables + my $composition = npg_tracking::glossary::composition->thaw( + slurp "$test_data_dir/29524#2.composition.json"); + my $row = $schema->resultset('BamFlagstats') + ->find_or_create_seq_composition($composition); + $check = npg_qc::autoqc::checks::meta->new($init); + throws_ok { $check->_results } qr/$expected found none/, 'no results - error'; + + my $create_qc_record = sub { + my ($file_name, $rs_name) = @_; + my $values = from_json(slurp "$test_data_dir/$file_name"); + delete $values->{'__CLASS__'}; + delete $values->{'composition'}; + delete $values->{'result_file_path'}; + $values->{'id_seq_composition'} = $row->id_seq_composition; + $schema->resultset($rs_name)->create($values); + }; + + # create a record in one of the result tables + $create_qc_record->('29524#2.bam_flagstats.json', 'BamFlagstats'); + + $init->{'qc_in'} = $test_data_dir; + + $check = npg_qc::autoqc::checks::meta->new($init); + throws_ok { $check->_results } + qr/$expected found results for bam_flagstats/, 'not all results - error'; + + # create records in all necessary tables plus one more + $create_qc_record->('29524#2.qX_yield.json', 'QXYield'); + $create_qc_record->('29524#2.bcfstats.json', 'Bcfstats'); + $create_qc_record->('29524#2.verify_bam_id.json', 'VerifyBamId'); + + + $check = npg_qc::autoqc::checks::meta->new($init); + lives_ok { $check->_results } 'no error - all expected results loaded'; + is_deeply ([sort keys %{$check->_results}], + [sort qw/bam_flagstats bcfstats verify_bam_id/], 'correct keys'); + for my $name (keys %{$check->_results}) { + my $result = $check->_results->{$name}; + like (ref $result, qr/\Anpg_qc::Schema::Result/, 'DBIx object retrieved'); + is ($check->_results->{$name}->class_name, $name, 'result type is correct'); + is ($result->composition->get_component(0)->tag_index, 2, 'tag index correct'); + } +}; + 1; diff --git a/t/data/autoqc/meta/29524#2.composition.json b/t/data/autoqc/meta/29524#2.composition.json new file mode 100644 index 000000000..eface0e40 --- /dev/null +++ b/t/data/autoqc/meta/29524#2.composition.json @@ -0,0 +1 @@ +{"__CLASS__":"npg_tracking::glossary::composition-88.2.1","components":[{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":1,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":2,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":3,"tag_index":2},{"__CLASS__":"npg_tracking::glossary::composition::component::illumina-88.2.1","id_run":29524,"position":4,"tag_index":2}]} \ No newline at end of file From a75577b32c43db42699cc03e370c3cd666b02c70 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Fri, 24 May 2019 16:53:51 +0100 Subject: [PATCH 09/23] evaluation --- MANIFEST | 1 + lib/npg_qc/autoqc/checks/meta.pm | 123 +++++++++++++++++++++-- lib/npg_qc/autoqc/results/meta.pm | 13 +++ t/60-autoqc-checks-meta.t | 122 +++++++++++++++++++--- t/data/autoqc/meta/samplesheet_29524.csv | 18 ++++ 5 files changed, 253 insertions(+), 24 deletions(-) create mode 100644 t/data/autoqc/meta/samplesheet_29524.csv diff --git a/MANIFEST b/MANIFEST index 06b2589ec..f3b74979d 100644 --- a/MANIFEST +++ b/MANIFEST @@ -424,6 +424,7 @@ t/data/autoqc/load/do_not_add_files_to_this_dir t/data/autoqc/load/some.json t/data/autoqc/meta/product_release.yml t/data/autoqc/meta/samplesheet_27483.csv +t/data/autoqc/meta/samplesheet_29524.csv t/data/autoqc/meta/29524#2.composition.json t/data/autoqc/meta/29524#17.bam_flagstats.json t/data/autoqc/meta/29524#2.bam_flagstats.json diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/meta.pm index 9fba978bb..bcb7e14c4 100644 --- a/lib/npg_qc/autoqc/checks/meta.pm +++ b/lib/npg_qc/autoqc/checks/meta.pm @@ -4,7 +4,8 @@ use Moose; use namespace::autoclean; use Carp; use Readonly; -use List::MoreUtils qw/any uniq/; +use List::MoreUtils qw/all any uniq/; +use English qw(-no_match_vars); use st::api::lims; use npg_qc::autoqc::qc_store; @@ -144,12 +145,42 @@ sub execute { my $self = shift; $self->can_run() or return; - $self->result->criteria($self->_criteria); + $self->result->pass($self->evaluate()); + # The next step is the conversion of a boolean outcome into + # mqc-compatible data structure. return; } +=head2 evaluate + +Main evaluation algorithm. Current implementation is very simple: +all criteria found in the product configuration file are assumed +to be equally essential. Therefore, a conjunction (AND) operator +is applied to a list of this criteria. + +Private methods and attributes of this class and the result class +implementation for this check are capable of supporting a more +complex algorithm should this become necessary in future. + +Returns 1 if all evaluated conditions were satisfied, otherise +returns 0. Sets the evaluation_results attribute of the results +object instance. + +=cut + +sub evaluate { + my $self = shift; + + my $emap = $self->_evaluate_expressions_array($self->_expressions); + while (my ($e, $o) = each %{$emap}) { + $self->result->evaluation_results()->{$e} = $o; + } + + return $self->_apply_operator([values %{$emap}], $CONJUNCTION_OP); +} + has '_lims' => ( isa => 'st::api::lims', is => 'ro', @@ -245,14 +276,9 @@ has '_result_class_names' => ( ); sub _build__result_class_names { my $self = shift; - - my @class_names = (); - foreach my $c ( @{$self->_expressions()} ) { - my ($class_name) = $c =~ /\A(?:\W+)?(\w+)[.]/xms; - $class_name or croak "Failed to infer class name from $c"; - push @class_names, $class_name; - } - @class_names = uniq sort @class_names; + my @class_names = uniq sort + map { _class_name_from_expression($_) } + @{$self->_expressions()}; return \@class_names; } @@ -315,6 +341,13 @@ sub _build__results { return \%h; } +sub _class_name_from_expression { + my $e = shift; + my ($class_name) = $e =~ /\A(?:\W+)?(\w+)[.]/xms; + $class_name or croak "Failed to infer class name from $e"; + return $class_name; +} + sub _traverse { my ($node, $expressions) = @_; @@ -336,6 +369,74 @@ sub _traverse { return; } +##### +# Given an array of expressions, evaluates them in the context of available +# autoqc results. Maps outcomes (as 1 or 0) to expressions and returns this +# hash. +# +sub _evaluate_expressions_array { + my ($self, $expressions) = @_; + + my $map = {}; + foreach my $e (@{$expressions}) { + $map->{$e} = $self->_evaluate_expression($e); + } + + return $map; +} + +##### +# Applies a logical operator to all array members. +# Defaults to aplying the conjunction operator. +# Returns 0 or 1. +# +sub _apply_operator { + my ($self, $outcomes, $operator) = @_; + + $operator ||= $CONJUNCTION_OP; + ($operator eq $CONJUNCTION_OP) or ($operator eq $DISJUNCTION_OP) + or croak "Unknown logical operator $operator"; + + my $outcome = $operator eq $CONJUNCTION_OP ? + all { $_ } @{$outcomes} : any { $_ } @{$outcomes}; + + return $outcome ? 1 : 0; +} + +##### +# Evaluates a single expression in the context of available autoqc results. +# Returns 0 or 1. +# +sub _evaluate_expression { + my ($self, $e) = @_; + + my $class_name = _class_name_from_expression($e); + my $obj = $self-> _results->{$class_name}; + # We should not get this far with an error in the configuration + # filr, but just in case... + $obj or croak "No autoqc result for evaluation of '$e'"; + + ##no critic (ValuesAndExpressions::RequireInterpolationOfMetachars) + my $replacement = q[$result->]; + ##use critic + my $perl_e = $e; + $perl_e =~ s/$class_name[.]/$replacement/xmsg; + + my $evaluator = sub { + my $result = shift; + ##no critic (BuiltinFunctions::ProhibitStringyEval) + my $o = eval $perl_e; # Evaluate Perl string expression + ##use critic + if ($EVAL_ERROR) { + my $err = $EVAL_ERROR; + croak "Error evaluating expression '$perl_e' derived from '$e': $err"; + } + return $o ? 1 : 0; + }; + + return $evaluator->($obj); +} + __PACKAGE__->meta->make_immutable(); 1; @@ -358,6 +459,8 @@ __END__ =item List::MoreUtils +=item English + =item st::api::lims =item npg_tracking::util::pipeline_config diff --git a/lib/npg_qc/autoqc/results/meta.pm b/lib/npg_qc/autoqc/results/meta.pm index d8c0e5cf0..bdbecde35 100644 --- a/lib/npg_qc/autoqc/results/meta.pm +++ b/lib/npg_qc/autoqc/results/meta.pm @@ -59,6 +59,19 @@ has 'criteria' => ( lazy_build => 1, ); +=head2 evaluation_results + +A hash of individual string expressions (keys) mapped to boolean outcomes of +their evaluation. An empty hash is a default. + +=cut + +has 'evaluation_results' => ( + isa => 'HashRef', + is => 'rw', + default => sub { return {} }, +); + __PACKAGE__->meta->make_immutable; 1; diff --git a/t/60-autoqc-checks-meta.t b/t/60-autoqc-checks-meta.t index 1d6e617dc..e989fb383 100644 --- a/t/60-autoqc-checks-meta.t +++ b/t/60-autoqc-checks-meta.t @@ -1,12 +1,13 @@ use strict; use warnings; -use Test::More tests => 5; +use Test::More tests => 7; use Test::Exception; use Test::Warn; use File::Temp qw/tempdir/; use File::Copy; -use Perl6::Slurp; -use JSON qw/from_json/; +use File::Slurp qw/read_file write_file/; +use JSON qw/from_json to_json/; +use List::MoreUtils qw/any/; use npg_testing::db; use npg_qc::autoqc::qc_store; @@ -21,8 +22,16 @@ my $conf_file_path = "$test_data_dir/product_release.yml"; local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = 't/data/autoqc/meta/samplesheet_27483.csv'; +my $criteria_list = [ + '( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95', + 'bam_flagstats.target_mapped_bases > 85_000_000_000', + 'bam_flagstats.target_percent_gt_coverage_threshold > 95', + 'verify_bam_id.freemix < 0.01', + '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02' +]; + subtest 'construction object, deciding whether to run' => sub { - plan tests => 18; + plan tests => 19; my $check = npg_qc::autoqc::checks::meta->new( conf_path => $test_data_dir, @@ -40,6 +49,8 @@ subtest 'construction object, deciding whether to run' => sub { 'No criteria defined in the product configuration file', 'reason logged'); lives_ok { $check->execute() } 'cannot run, but execute method runs OK'; + is ($check->result->pass, undef, + 'pass atttribute of the result object is ndefined'); $check = npg_qc::autoqc::checks::meta->new( conf_path => "$test_data_dir/no_robo", @@ -76,14 +87,7 @@ subtest 'construction object, deciding whether to run' => sub { 'can_run is accompanied by warnings'; ok ($can_run, 'can_run returns true'); ok (!$check->result->comments, 'No comments logged'); - my $expected_criteria = {'and' => [ - '( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95', - 'bam_flagstats.target_mapped_bases > 85_000_000_000', - 'bam_flagstats.target_percent_gt_coverage_threshold > 95', - 'verify_bam_id.freemix < 0.01', - '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02' - ]}; - is_deeply ($check->_criteria, $expected_criteria, 'criteria parsed correctly'); + is_deeply ($check->_criteria, {'and' => $criteria_list}, 'criteria parsed correctly'); $check = npg_qc::autoqc::checks::meta->new( conf_path => "$test_data_dir/error1", @@ -213,7 +217,7 @@ subtest 'finding results - database' => sub { # create composition, but not records in the result tables my $composition = npg_tracking::glossary::composition->thaw( - slurp "$test_data_dir/29524#2.composition.json"); + read_file "$test_data_dir/29524#2.composition.json"); my $row = $schema->resultset('BamFlagstats') ->find_or_create_seq_composition($composition); $check = npg_qc::autoqc::checks::meta->new($init); @@ -221,7 +225,7 @@ subtest 'finding results - database' => sub { my $create_qc_record = sub { my ($file_name, $rs_name) = @_; - my $values = from_json(slurp "$test_data_dir/$file_name"); + my $values = from_json(read_file "$test_data_dir/$file_name"); delete $values->{'__CLASS__'}; delete $values->{'composition'}; delete $values->{'result_file_path'}; @@ -253,6 +257,96 @@ subtest 'finding results - database' => sub { like (ref $result, qr/\Anpg_qc::Schema::Result/, 'DBIx object retrieved'); is ($check->_results->{$name}->class_name, $name, 'result type is correct'); is ($result->composition->get_component(0)->tag_index, 2, 'tag index correct'); + } +}; + +subtest 'single expression evaluation' => sub { + plan tests => 10; + + local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + 't/data/autoqc/meta/samplesheet_29524.csv'; + my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; + + my $criteria = [ + '( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95', + 'bam_flagstats.target_mapped_bases > 85_000_000_000', + 'bam_flagstats.target_percent_gt_coverage_threshold > 95', + 'verify_bam_id.freemix < 0.01', + '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02' + ]; + + my $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $test_data_dir, + rpt_list => $rpt_list); + + throws_ok {$check->_evaluate_expression('verify_bam.freemix < 0.01')} + qr/No autoqc result for evaluation of/, + 'error if check name is unknown'; + throws_ok {$check->_evaluate_expression('verify_bam_id.freemix_free < 0.01')} + qr/Can't locate object method \"freemix_free\"/, + 'error if method name is unknown'; + + for my $c (@{$criteria}) { + lives_and { is $check->_evaluate_expression($c), 1 } "pass for $c"; + } + + is ($check->_evaluate_expression('verify_bam_id.freemix > 0.01'), 0, + 'negative outcome'); + is ($check->_evaluate_expression('bam_flagstats.target_mapped_bases > 85_000_000_000_000'), 0, + 'negative outcome'); + is ($check->_evaluate_expression( + '( bcfstats.genotypes_nrd_dividend * bcfstats.genotypes_nrd_divisor ) < 0.02'), 0, + 'negative outcome'); +}; + +subtest 'evaluation within the execute method' => sub { + plan tests => 12; + + local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + 't/data/autoqc/meta/samplesheet_29524.csv'; + my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; + + my $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $dir, + rpt_list => $rpt_list); + + lives_ok { $check->execute } 'execute method runs OK'; + is ($check->result->pass, 1, 'result pass attribute is set to 1'); + my %expected = map { $_ => 1 } @{$criteria_list}; + is_deeply ($check->result->evaluation_results(), \%expected, + 'evaluation results are saved'); + + my $target = "$dir/29524#2.bam_flagstats.json"; + + my $failures = {}; + $failures->{3} = [qw/target_mapped_bases/]; + $failures->{7} = [qw/target_mapped_bases target_percent_gt_coverage_threshold/]; + $failures->{17} = [qw/target_percent_gt_coverage_threshold/]; + + for my $index (qw/3 7 17/) { + + my $f = '29524#' . $index . '.bam_flagstats.json'; + my $values = from_json(read_file "$test_data_dir/$f"); + for my $component (@{$values->{composition}->{components}}) { + $component->{tag_index} = 2; + } + write_file($target, to_json($values)); + + $check = npg_qc::autoqc::checks::meta->new( + conf_path => $test_data_dir, + qc_in => $dir, + rpt_list => $rpt_list); + lives_ok { $check->execute } 'execute method runs OK'; + is ($check->result->pass, 0, 'result pass attribute is set to 0'); + my $e = {}; + my @failed = @{$failures->{$index}}; + for my $c (@{$criteria_list}) { + $e->{$c} = (any { $c =~ /$_/ } @failed) ? 0 : 1; + } + + is_deeply ($check->result->evaluation_results(), $e, 'evaluation results are saved'); } }; diff --git a/t/data/autoqc/meta/samplesheet_29524.csv b/t/data/autoqc/meta/samplesheet_29524.csv new file mode 100644 index 000000000..f0a2cfed0 --- /dev/null +++ b/t/data/autoqc/meta/samplesheet_29524.csv @@ -0,0 +1,18 @@ +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Lane,Sample_ID,Sample_Name,GenomeFolder,Index,Index2,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +1,25385022,7855900,,TGCGGCGT,CCTCGGTA,,HiSeqX PCR free,TGCGGCGT,CCTCGGTA,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088543,0,25385022,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855900,4095118,7855900,,,1277994,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,1, +1,25385034,7855901,,CATAATAC,TTCTAACG,,HiSeqX PCR free,CATAATAC,TTCTAACG,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088543,0,25385034,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855901,4095119,7855901,,,5455485,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,2, +1,25385046,7855902,,GATCTATC,ATGAGGCT,,HiSeqX PCR free,GATCTATC,ATGAGGCT,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088543,0,25385046,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855902,4095120,7855902,,,2658259,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,3, +1,21210572,phiX_for_spiked_buffers,,ACAACGCAATC,,,,ACAACGCAATC,,,,,,,1,0,26088543,0,21210572,,10847,,,,standard,,,,,,,0,,,1255141,phiX_for_spiked_buffers,,PhiX (Sanger-SNPs),,888,,1,0,0,None,198,Illumina Controls, ,0,,888, +2,25385022,7855900,,TGCGGCGT,CCTCGGTA,,HiSeqX PCR free,TGCGGCGT,CCTCGGTA,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088544,0,25385022,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855900,4095118,7855900,,,1277994,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,1, +2,25385034,7855901,,CATAATAC,TTCTAACG,,HiSeqX PCR free,CATAATAC,TTCTAACG,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088544,0,25385034,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855901,4095119,7855901,,,5455485,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,2, +2,25385046,7855902,,GATCTATC,ATGAGGCT,,HiSeqX PCR free,GATCTATC,ATGAGGCT,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088544,0,25385046,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855902,4095120,7855902,,,2658259,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,3, +2,21210572,phiX_for_spiked_buffers,,ACAACGCAATC,,,,ACAACGCAATC,,,,,,,1,0,26088544,0,21210572,,10847,,,,standard,,,,,,,0,,,1255141,phiX_for_spiked_buffers,,PhiX (Sanger-SNPs),,888,,1,0,0,None,198,Illumina Controls, ,0,,888, +3,25385022,7855900,,TGCGGCGT,CCTCGGTA,,HiSeqX PCR free,TGCGGCGT,CCTCGGTA,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088545,0,25385022,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855900,4095118,7855900,,,1277994,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,1, +3,25385034,7855901,,CATAATAC,TTCTAACG,,HiSeqX PCR free,CATAATAC,TTCTAACG,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088545,0,25385034,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855901,4095119,7855901,,,5455485,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,2, +3,25385046,7855902,,GATCTATC,ATGAGGCT,,HiSeqX PCR free,GATCTATC,ATGAGGCT,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088545,0,25385046,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855902,4095120,7855902,,,2658259,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,3, +3,21210572,phiX_for_spiked_buffers,,ACAACGCAATC,,,,ACAACGCAATC,,,,,,,1,0,26088545,0,21210572,,10847,,,,standard,,,,,,,0,,,1255141,phiX_for_spiked_buffers,,PhiX (Sanger-SNPs),,888,,1,0,0,None,198,Illumina Controls, ,0,,888, +4,25385022,7855900,,TGCGGCGT,CCTCGGTA,,HiSeqX PCR free,TGCGGCGT,CCTCGGTA,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088546,0,25385022,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855900,4095118,7855900,,,1277994,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,1, +4,25385034,7855901,,CATAATAC,TTCTAACG,,HiSeqX PCR free,CATAATAC,TTCTAACG,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088546,0,25385034,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855901,4095119,7855901,,,5455485,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,2, +4,25385046,7855902,,GATCTATC,ATGAGGCT,,HiSeqX PCR free,GATCTATC,ATGAGGCT,ssss@sanger.ac.uk,,ssss@sanger.ac.uk,ssss@sanger.ac.uk,,0,0,26088546,0,25385046,,9606,T4600,,,standard,,,from:450 to:450,,,Homo sapiens,0,,7855902,4095120,7855902,,,2658259,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,3, +4,21210572,phiX_for_spiked_buffers,,ACAACGCAATC,,,,ACAACGCAATC,,,,,,,1,0,26088546,0,21210572,,10847,,,,standard,,,,,,,0,,,1255141,phiX_for_spiked_buffers,,PhiX (Sanger-SNPs),,888,,1,0,0,None,198,Illumina Controls, ,0,,888, From 8b32f6bfee2f8d981b92ec8c7989439256060724 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Wed, 29 May 2019 16:28:15 +0100 Subject: [PATCH 10/23] saving qc outcome --- lib/npg_qc/Schema/Mqc/OutcomeDict.pm | 35 +++++++++++++++++++--- lib/npg_qc/autoqc/checks/meta.pm | 33 ++++++++++++++++++-- lib/npg_qc/autoqc/results/meta.pm | 10 +++++++ t/50-schema-result-MqcLibraryOutcomeDict.t | 24 ++++++++++++++- t/60-autoqc-checks-meta.t | 17 ++++++++--- 5 files changed, 107 insertions(+), 12 deletions(-) diff --git a/lib/npg_qc/Schema/Mqc/OutcomeDict.pm b/lib/npg_qc/Schema/Mqc/OutcomeDict.pm index 4f68f1679..0973094f6 100644 --- a/lib/npg_qc/Schema/Mqc/OutcomeDict.pm +++ b/lib/npg_qc/Schema/Mqc/OutcomeDict.pm @@ -2,11 +2,15 @@ package npg_qc::Schema::Mqc::OutcomeDict; use Moose::Role; use Readonly; +use Carp; our $VERSION = '0'; Readonly::Scalar my $FINAL => 'final'; Readonly::Scalar my $PRELIMINARY => 'preliminary'; +Readonly::Scalar my $ACCEPTED => 'Accepted'; +Readonly::Scalar my $REJECTED => 'Rejected'; +Readonly::Scalar my $UNDECIDED => 'Undecided'; sub is_final_outcome_description { my ($self, $desc) = @_; @@ -20,12 +24,12 @@ sub is_final_outcome { sub is_accepted { my $self = shift; - return $self->short_desc =~ /\AAccepted/smx; #The short description includes the word accepted. + return $self->short_desc =~ /\A $ACCEPTED/smx; #The short description includes the word accepted. } sub is_rejected { my $self = shift; - return $self->short_desc =~ /\ARejected/smx; #The short description includes the word rejected. + return $self->short_desc =~ /\A $REJECTED/smx; #The short description includes the word rejected. } sub is_final_accepted { @@ -35,7 +39,7 @@ sub is_final_accepted { sub is_undecided { my $self = shift; - return $self->short_desc =~ /\AUndecided/smx; + return $self->short_desc =~ /\A $UNDECIDED/smx; } sub pk_value { @@ -59,6 +63,15 @@ sub matching_final_short_desc { return $desc; } +sub generate_short_description { + my ($self, $is_final, $is_accepted) = @_; + + defined $is_final or croak 'Final flag should be defined'; + my $decision = $is_accepted ? $ACCEPTED : + (defined $is_accepted ? $REJECTED : $UNDECIDED); + return join q[ ], $decision, $is_final ? $FINAL : $PRELIMINARY; +} + no Moose::Role; 1; @@ -133,6 +146,18 @@ __END__ print $dict_row->short_desc; # Rejected final print $dict_row->matching_final_short_desc(); # Rejected final +=head2 generate_short_description + + Package-level method for generating descriptions matching short + descriptions in the dictionary tables. + + my $is_final = 1; + my $is_accepted = 1; + __PACKAGE__->generate_short_description($is_final, $is_accepted); + # returns 'Accepted final' + __PACKAGE__->generate_short_description($is_final); + # returns 'Undecided final' + =head1 DIAGNOSTICS =head1 CONFIGURATION AND ENVIRONMENT @@ -145,6 +170,8 @@ __END__ =item Readonly +=item Carp + =back =head1 INCOMPATIBILITIES @@ -157,7 +184,7 @@ Jaime Tovar jmtc@sanger.ac.uk =head1 LICENSE AND COPYRIGHT -Copyright (C) 2017 Genome Research Ltd +Copyright (C) 2019 Genome Research Ltd This file is part of NPG. diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/meta.pm index bcb7e14c4..139ba0687 100644 --- a/lib/npg_qc/autoqc/checks/meta.pm +++ b/lib/npg_qc/autoqc/checks/meta.pm @@ -6,9 +6,11 @@ use Carp; use Readonly; use List::MoreUtils qw/all any uniq/; use English qw(-no_match_vars); +use DateTime; use st::api::lims; use npg_qc::autoqc::qc_store; +use npg_qc::Schema::Mqc::OutcomeDict; extends 'npg_qc::autoqc::checks::check'; with 'npg_tracking::util::pipeline_config'; @@ -22,6 +24,8 @@ Readonly::Scalar my $ROBO_KEY => q[robo_qc]; Readonly::Scalar my $LIBRARY_TYPE_KEY => q[library_type]; Readonly::Scalar my $CRITERIA_KEY => q[acceptance_criteria]; +Readonly::Scalar my $TIMESTAMP_FORMAT_WOFFSET => q[%Y-%m-%dT%T%z]; + ## no critic (Documentation::RequirePodAtEnd) =head1 NAME @@ -146,9 +150,8 @@ sub execute { $self->can_run() or return; $self->result->criteria($self->_criteria); - $self->result->pass($self->evaluate()); - # The next step is the conversion of a boolean outcome into - # mqc-compatible data structure. + $self->result->pass($self->evaluate); + $self->result->qc_outcome($self->_generate_qc_outcome); return; } @@ -168,6 +171,11 @@ Returns 1 if all evaluated conditions were satisfied, otherise returns 0. Sets the evaluation_results attribute of the results object instance. +In future we might have both acceptance and rejection criteria +defined. When neither of these sets of criteria is satisfied, this +method should return an undefined value, which will be translated +to the 'Undecided' qc outcome. + =cut sub evaluate { @@ -437,6 +445,23 @@ sub _evaluate_expression { return $evaluator->($obj); } +sub _generate_qc_outcome { + my $self = shift; + + ##### + # Any of Accepted, Rejected, Undecided outcomes can be returned here + my $outcome = npg_qc::Schema::Mqc::OutcomeDict->generate_short_description( + $self->final_qc_outcome ? 1 : 0, $self->result->pass); + # Temporary code, will be replaced by our own package for DateTime + # object serialization. + my $timestamp = DateTime->now(time_zone => q[local]) + ->strftime($TIMESTAMP_FORMAT_WOFFSET); + + return { mqc_outcome => $outcome, + timestamp => $timestamp, + username => $ROBO_KEY }; +} + __PACKAGE__->meta->make_immutable(); 1; @@ -461,6 +486,8 @@ __END__ =item English +=item Date::Time + =item st::api::lims =item npg_tracking::util::pipeline_config diff --git a/lib/npg_qc/autoqc/results/meta.pm b/lib/npg_qc/autoqc/results/meta.pm index bdbecde35..f3e8f75c9 100644 --- a/lib/npg_qc/autoqc/results/meta.pm +++ b/lib/npg_qc/autoqc/results/meta.pm @@ -72,6 +72,16 @@ has 'evaluation_results' => ( default => sub { return {} }, ); +=head2 qc_outcome + +=cut + +has 'qc_outcome' => ( + isa => 'HashRef', + is => 'rw', + default => sub { return {} }, +); + __PACKAGE__->meta->make_immutable; 1; diff --git a/t/50-schema-result-MqcLibraryOutcomeDict.t b/t/50-schema-result-MqcLibraryOutcomeDict.t index 03c7a40d8..ba5012a74 100644 --- a/t/50-schema-result-MqcLibraryOutcomeDict.t +++ b/t/50-schema-result-MqcLibraryOutcomeDict.t @@ -1,6 +1,7 @@ use strict; use warnings; -use Test::More tests => 28; +use Test::More tests => 37; +use Test::Exception; use Moose::Meta::Class; use npg_testing::db; @@ -42,5 +43,26 @@ ok (!$rows[2]->is_final_accepted, 'accepted & final outcome check returns false' is ($rows[2]->matching_final_short_desc(), 'Undecided final', 'matching final'); is ($rows[2]->pk_value(), $rows[2]->id_mqc_library_outcome, 'primary key value'); +throws_ok { $rows[0]->generate_short_description() } +qr/Final flag should be defined/, 'error if final flag undefined'; + +is ($rows[0]->generate_short_description(1, 1), + 'Accepted final', 'description for final accepted'); +is ($rows[0]->generate_short_description(1, 0), + 'Rejected final', 'description for final rejected'); +is ($rows[0]->generate_short_description(1), + 'Undecided final', 'description for final undecided'); +is ($rows[0]->generate_short_description(1, undef), + 'Undecided final', 'description for final undecided'); + +is ($rows[0]->generate_short_description(0, 'accepted'), + 'Accepted preliminary', 'description for prelim accepted'); +is ($rows[0]->generate_short_description(0, q[]), + 'Rejected preliminary', 'description for prelim rejected'); +is ($rows[0]->generate_short_description(0), + 'Undecided preliminary', 'description for prelim undecided'); +is ($rows[0]->generate_short_description(0, undef), + 'Undecided preliminary', 'description for prelim undecided'); + 1; diff --git a/t/60-autoqc-checks-meta.t b/t/60-autoqc-checks-meta.t index e989fb383..8caaf7d2d 100644 --- a/t/60-autoqc-checks-meta.t +++ b/t/60-autoqc-checks-meta.t @@ -301,7 +301,7 @@ subtest 'single expression evaluation' => sub { }; subtest 'evaluation within the execute method' => sub { - plan tests => 12; + plan tests => 24; local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = 't/data/autoqc/meta/samplesheet_29524.csv'; @@ -317,6 +317,10 @@ subtest 'evaluation within the execute method' => sub { my %expected = map { $_ => 1 } @{$criteria_list}; is_deeply ($check->result->evaluation_results(), \%expected, 'evaluation results are saved'); + my $outcome = $check->result->qc_outcome; + is ($outcome->{'mqc_outcome'} , 'Accepted preliminary', 'correct outcome string'); + is ($outcome->{'username'}, 'robo_qc', 'correct process id'); + ok ($outcome->{'timestamp'}, 'timestamp saved'); my $target = "$dir/29524#2.bam_flagstats.json"; @@ -335,9 +339,10 @@ subtest 'evaluation within the execute method' => sub { write_file($target, to_json($values)); $check = npg_qc::autoqc::checks::meta->new( - conf_path => $test_data_dir, - qc_in => $dir, - rpt_list => $rpt_list); + final_qc_outcome => 1, + conf_path => $test_data_dir, + qc_in => $dir, + rpt_list => $rpt_list); lives_ok { $check->execute } 'execute method runs OK'; is ($check->result->pass, 0, 'result pass attribute is set to 0'); my $e = {}; @@ -347,6 +352,10 @@ subtest 'evaluation within the execute method' => sub { } is_deeply ($check->result->evaluation_results(), $e, 'evaluation results are saved'); + $outcome = $check->result->qc_outcome; + is ($outcome->{'mqc_outcome'} , 'Rejected final', 'correct outcome string'); + is ($outcome->{'username'}, 'robo_qc', 'correct process id'); + ok ($outcome->{'timestamp'}, 'timestamp saved'); } }; From dda14a2cdef0e948c9da3fc27c0f3ea398df15d0 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Wed, 29 May 2019 16:57:28 +0100 Subject: [PATCH 11/23] tests for the result object --- lib/npg_qc/autoqc/results/meta.pm | 4 +++- t/60-autoqc-results-meta.t | 26 +++++++++++++++++++++++++- 2 files changed, 28 insertions(+), 2 deletions(-) diff --git a/lib/npg_qc/autoqc/results/meta.pm b/lib/npg_qc/autoqc/results/meta.pm index f3e8f75c9..3cea23f80 100644 --- a/lib/npg_qc/autoqc/results/meta.pm +++ b/lib/npg_qc/autoqc/results/meta.pm @@ -2,7 +2,9 @@ package npg_qc::autoqc::results::meta; use Moose; use namespace::autoclean; -extends qw(npg_qc::autoqc::results::result); + +extends 'npg_qc::autoqc::results::result'; +with 'npg_qc::autoqc::role::result'; our $VERSION = '0'; diff --git a/t/60-autoqc-results-meta.t b/t/60-autoqc-results-meta.t index e7b393e80..1e0e68ebf 100644 --- a/t/60-autoqc-results-meta.t +++ b/t/60-autoqc-results-meta.t @@ -1,8 +1,32 @@ use strict; use warnings; -use Test::More tests => 1; +use Test::More tests => 3; use Test::Exception; +use npg_tracking::glossary::composition::component::illumina; +use npg_tracking::glossary::composition; + use_ok('npg_qc::autoqc::results::meta'); +my $composition = npg_tracking::glossary::composition->new( + components => [ + npg_tracking::glossary::composition::component::illumina->new( + id_run => 1, position => 1, tag_index => 3, + ), + npg_tracking::glossary::composition::component::illumina->new( + id_run => 1, position => 2, tag_index => 3, + ) + ] +); + +my $r = npg_qc::autoqc::results::meta->new(composition => $composition); +isa_ok ($r, 'npg_qc::autoqc::results::meta'); +$r->qc_outcome({mqc_outcome => 'Accepted_final', + timestamp => '2019-05-23T17:11:31+0100', + username => 'robo_qc'}); +$r->criteria({'and' => [qw/expressionA expressionB/]}); +$r->evaluation_results({expressionA => 1, expressionB => 1}); + +lives_ok { $r->freeze } 'object can be serialized'; + 1; From 93a1a330e293c335b6fe3a41beef92cbb03a2be4 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Thu, 30 May 2019 10:58:20 +0100 Subject: [PATCH 12/23] docs extended to describe capturing rejection criteria --- lib/npg_qc/autoqc/checks/meta.pm | 57 +++++++++++++++++++++++++++---- lib/npg_qc/autoqc/results/meta.pm | 42 ++++++++--------------- 2 files changed, 64 insertions(+), 35 deletions(-) diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/meta.pm index 139ba0687..c5b409066 100644 --- a/lib/npg_qc/autoqc/checks/meta.pm +++ b/lib/npg_qc/autoqc/checks/meta.pm @@ -22,7 +22,7 @@ Readonly::Scalar my $DISJUNCTION_OP => q[or]; Readonly::Scalar my $ROBO_KEY => q[robo_qc]; Readonly::Scalar my $LIBRARY_TYPE_KEY => q[library_type]; -Readonly::Scalar my $CRITERIA_KEY => q[acceptance_criteria]; +Readonly::Scalar my $ACCEPTANCE_CRITERIA_KEY => q[acceptance_criteria]; Readonly::Scalar my $TIMESTAMP_FORMAT_WOFFSET => q[%Y-%m-%dT%T%z]; @@ -39,6 +39,8 @@ npg_qc::autoqc::checks::meta =head1 DESCRIPTION +=head2 Overview + This checks evaluates the results of other autoqc checks against a pre-defined set of criteria. @@ -58,6 +60,8 @@ outcome is also marked as 'Final', otherwise it's marked as By default the final_qc_outcome flag is false and the produced outcomes are preliminary. +=head2 Retrieval of autoqc results to be evaluated + We will not try to second-guess here what is and what is not a product. Therefore, it is possible to invoke this check on any entity apart from, possibly, a run. At run time an attempt @@ -78,6 +82,47 @@ npg_qc::autoqc::qc_store class is used to load results. In contrast to npg_qc::autoqc::qc_store, if the database retrieval is enabled, no fall back to a search on a file system is done. +=head2 Record of the evaluation criteria + +The result object for this check records evaluation criteria +in a form that would not require any additional information to +repeate the evaluation as it was done at the time the check was +run. + +All boolean operators are listed explicitly. The top-level +expression is either a conjunction or disjunction performed on +a list of expressions, each of wich can be, in turn, either a +math expression or a further boolean expression on a list of +expressions. + +Examples: + + Assuming a = 2 and b = 5, + {'and' => ["a-1 < 0", "b+3 > 10"]} translates to + (a-1 > 0) && (b+3 > 10) and evaluates to false, while + {'or' => ["a-1 > 0", "b+3 > 10"]} translates to + (a-1 > 0) || (b+3 > 10) and evaluates to true. + + Assuming additionally c = 3 and d = 1, + {'and' => ["a-1 > 0", "b+3 > 5", {'or' => ["c-d > 0", "c-d < -1"]}]} + translates to + (a-1 > 0) && (b+3 > 5) && ((c-d > 0) || (c-d < -1)) + and evaluates to true. + +if both acceptance and rejection criteria have to be accomodated, this +can be done in the following way (note the negation operator): + + { + 'and' => [ + {'not' => {'or' => [$rejection_expr1, $rejection_expr2]}}, + {'and' => [$acceptance_expr1, $acceptance_expr2]}} + ] + } + +The outcome of the evaluation will be either true or false. The undecided +outcome can be assigned if the overall expression evaluates to false and +a full evaluation was performed (ie the acceptance branch was evaluated). + =head1 SUBROUTINES/METHODS =head2 use_db @@ -172,9 +217,7 @@ returns 0. Sets the evaluation_results attribute of the results object instance. In future we might have both acceptance and rejection criteria -defined. When neither of these sets of criteria is satisfied, this -method should return an undefined value, which will be translated -to the 'Undecided' qc outcome. +defined, adding an undefined value as a legitimate outcome. =cut @@ -237,7 +280,7 @@ sub _build__criteria { # foreach my $criteria_set (@{$study_config->{$ROBO_KEY}}) { $criteria_set->{$LIBRARY_TYPE_KEY} or croak "$LIBRARY_TYPE_KEY key is missing"; - $criteria_set->{$CRITERIA_KEY} or croak "$CRITERIA_KEY key is missing"; + $criteria_set->{$ACCEPTANCE_CRITERIA_KEY} or croak "$ACCEPTANCE_CRITERIA_KEY key is missing"; if (any {$_ eq $lib_type} map { lc } @{$criteria_set->{$LIBRARY_TYPE_KEY}}) { ##### # A very simple criteria format - a list of strings - is used for now. @@ -246,7 +289,7 @@ sub _build__criteria { # that should give the result of the evaluation. Therefore, at the # moment it is safe to collect all criteria in a single list. # - push @criteria, @{$criteria_set->{$CRITERIA_KEY}}; + push @criteria, @{$criteria_set->{$ACCEPTANCE_CRITERIA_KEY}}; } } if (@criteria) { @@ -452,7 +495,7 @@ sub _generate_qc_outcome { # Any of Accepted, Rejected, Undecided outcomes can be returned here my $outcome = npg_qc::Schema::Mqc::OutcomeDict->generate_short_description( $self->final_qc_outcome ? 1 : 0, $self->result->pass); - # Temporary code, will be replaced by our own package for DateTime + # Temporary code, should use functions from our own package for DateTime # object serialization. my $timestamp = DateTime->now(time_zone => q[local]) ->strftime($TIMESTAMP_FORMAT_WOFFSET); diff --git a/lib/npg_qc/autoqc/results/meta.pm b/lib/npg_qc/autoqc/results/meta.pm index 3cea23f80..aa8d466ee 100644 --- a/lib/npg_qc/autoqc/results/meta.pm +++ b/lib/npg_qc/autoqc/results/meta.pm @@ -25,57 +25,43 @@ npg_qc::autoqc::results::meta =cut has 'library_type' => ( - isa => 'Str', - is => 'rw', + isa => 'Str', + is => 'rw', ); =head2 criteria A read-write hash reference attribute representing evaluation criteria in a form that would not require any additional information to repeate -the evaluation as it was done at the time the check was run. - -All boolean operators are listed explicitly. The top-level expression -is either a conjunction or disjunction performed on a list of -expressions, each of wich can be, in turn, either a math expression or -a conjunction or disjunction. - -Examples: - - Assuming a = 2 and b = 5, - {'and' => ["a-1 < 0", "b+3 > 10"]} translates to - (a-1 > 0) && (b+3 > 10) and evaluates to false, while - {'or' => ["a-1 > 0", "b+3 > 10"]} translates to - (a-1 > 0) || (b+3 > 10) and evaluates to true. - - Assuming additionally c = 3 and d = 1, - {'and' => ["a-1 > 0", "b+3 > 5", {'or' => ["c-d > 0", "c-d < -1"]}]} - translates to - (a-1 > 0) && (b+3 > 5) && ((c-d > 0) || (c-d < -1)) and evaluates to true. +the evaluation as it was done at the time the check was run. An empty +hash is default. =cut has 'criteria' => ( - isa => 'HashRef', - is => 'rw', - lazy_build => 1, + isa => 'HashRef', + is => 'rw', + default => sub { return {} }, ); =head2 evaluation_results A hash of individual string expressions (keys) mapped to boolean outcomes of -their evaluation. An empty hash is a default. +their evaluation. An empty hash is default. =cut has 'evaluation_results' => ( - isa => 'HashRef', - is => 'rw', - default => sub { return {} }, + isa => 'HashRef', + is => 'rw', + default => sub { return {} }, ); =head2 qc_outcome +A hash reference with information necessary for creating a record in the +mqc_library_outcome_ent table of the QC database. An empty hash is default. + =cut has 'qc_outcome' => ( From d5728f6b42142125181b9e2437f7ed4c571d806e Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Mon, 3 Jun 2019 10:14:37 +0100 Subject: [PATCH 13/23] use RFC 3339 compliant timestamp --- Build.PL | 1 + lib/npg_qc/autoqc/checks/meta.pm | 11 ++++------- 2 files changed, 5 insertions(+), 7 deletions(-) diff --git a/Build.PL b/Build.PL index d9c2244d9..b466b9837 100644 --- a/Build.PL +++ b/Build.PL @@ -75,6 +75,7 @@ my $requires = { 'URI::Escape' => 0, 'Vcf' => 0, + 'WTSI::DNAP::Utilities::Timestamp' => 0, 'npg_common::Alignment' => 0, 'npg_common::extractor::fastq' => 0, 'npg_common::roles::software_location' => 0, diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/meta.pm index c5b409066..c184e25e7 100644 --- a/lib/npg_qc/autoqc/checks/meta.pm +++ b/lib/npg_qc/autoqc/checks/meta.pm @@ -5,9 +5,10 @@ use namespace::autoclean; use Carp; use Readonly; use List::MoreUtils qw/all any uniq/; -use English qw(-no_match_vars); +use English qw/-no_match_vars/; use DateTime; +use WTSI::DNAP::Utilities::Timestamp qw/create_current_timestamp/; use st::api::lims; use npg_qc::autoqc::qc_store; use npg_qc::Schema::Mqc::OutcomeDict; @@ -495,13 +496,9 @@ sub _generate_qc_outcome { # Any of Accepted, Rejected, Undecided outcomes can be returned here my $outcome = npg_qc::Schema::Mqc::OutcomeDict->generate_short_description( $self->final_qc_outcome ? 1 : 0, $self->result->pass); - # Temporary code, should use functions from our own package for DateTime - # object serialization. - my $timestamp = DateTime->now(time_zone => q[local]) - ->strftime($TIMESTAMP_FORMAT_WOFFSET); return { mqc_outcome => $outcome, - timestamp => $timestamp, + timestamp => create_current_timestamp(), username => $ROBO_KEY }; } @@ -529,7 +526,7 @@ __END__ =item English -=item Date::Time +=item WTSI::DNAP::Utilities::Timestamp =item st::api::lims From 2e4710f06a8d6f978ed7f8ef4095026458d56948 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Mon, 3 Jun 2019 11:14:14 +0100 Subject: [PATCH 14/23] check renamed --- MANIFEST | 32 +++++------ .../autoqc/checks/{meta.pm => review.pm} | 6 +- .../autoqc/results/{meta.pm => review.pm} | 4 +- ...hecks-meta.t => 60-autoqc-checks-review.t} | 56 +++++++++---------- t/60-autoqc-results-collection.t | 1 + ...ults-meta.t => 60-autoqc-results-review.t} | 6 +- .../29524#17.bam_flagstats.json | 0 .../29524#2.bam_flagstats.json | 0 .../{meta => review}/29524#2.bcfstats.json | 0 .../{meta => review}/29524#2.composition.json | 0 .../{meta => review}/29524#2.qX_yield.json | 0 .../29524#2.verify_bam_id.json | 0 .../29524#2_phix.bam_flagstats.json | 0 .../29524#3.bam_flagstats.json | 0 .../29524#7.bam_flagstats.json | 0 .../error1/product_release.yml | 0 .../error2/product_release.yml | 0 .../no_criteria/product_release.yml | 0 .../no_robo/product_release.yml | 0 .../{meta => review}/product_release.yml | 0 .../{meta => review}/samplesheet_27483.csv | 0 .../{meta => review}/samplesheet_29524.csv | 0 .../with_criteria/product_release.yml | 0 23 files changed, 53 insertions(+), 52 deletions(-) rename lib/npg_qc/autoqc/checks/{meta.pm => review.pm} (99%) rename lib/npg_qc/autoqc/results/{meta.pm => review.pm} (96%) rename t/{60-autoqc-checks-meta.t => 60-autoqc-checks-review.t} (89%) rename t/{60-autoqc-results-meta.t => 60-autoqc-results-review.t} (83%) rename t/data/autoqc/{meta => review}/29524#17.bam_flagstats.json (100%) rename t/data/autoqc/{meta => review}/29524#2.bam_flagstats.json (100%) rename t/data/autoqc/{meta => review}/29524#2.bcfstats.json (100%) rename t/data/autoqc/{meta => review}/29524#2.composition.json (100%) rename t/data/autoqc/{meta => review}/29524#2.qX_yield.json (100%) rename t/data/autoqc/{meta => review}/29524#2.verify_bam_id.json (100%) rename t/data/autoqc/{meta => review}/29524#2_phix.bam_flagstats.json (100%) rename t/data/autoqc/{meta => review}/29524#3.bam_flagstats.json (100%) rename t/data/autoqc/{meta => review}/29524#7.bam_flagstats.json (100%) rename t/data/autoqc/{meta => review}/error1/product_release.yml (100%) rename t/data/autoqc/{meta => review}/error2/product_release.yml (100%) rename t/data/autoqc/{meta => review}/no_criteria/product_release.yml (100%) rename t/data/autoqc/{meta => review}/no_robo/product_release.yml (100%) rename t/data/autoqc/{meta => review}/product_release.yml (100%) rename t/data/autoqc/{meta => review}/samplesheet_27483.csv (100%) rename t/data/autoqc/{meta => review}/samplesheet_29524.csv (100%) rename t/data/autoqc/{meta => review}/with_criteria/product_release.yml (100%) diff --git a/MANIFEST b/MANIFEST index f3b74979d..2306bbaee 100644 --- a/MANIFEST +++ b/MANIFEST @@ -28,10 +28,10 @@ lib/npg_qc/autoqc/checks/gc_fraction.pm lib/npg_qc/autoqc/checks/genotype.pm lib/npg_qc/autoqc/checks/genotype_call.pm lib/npg_qc/autoqc/checks/insert_size.pm -lib/npg_qc/autoqc/checks/meta.pm lib/npg_qc/autoqc/checks/pulldown_metrics.pm lib/npg_qc/autoqc/checks/qX_yield.pm lib/npg_qc/autoqc/checks/ref_match.pm +lib/npg_qc/autoqc/checks/review.pm lib/npg_qc/autoqc/checks/rna_seqc.pm lib/npg_qc/autoqc/checks/samtools_stats.pm lib/npg_qc/autoqc/checks/sequence_error.pm @@ -59,11 +59,11 @@ lib/npg_qc/autoqc/results/gc_fraction.pm lib/npg_qc/autoqc/results/genotype.pm lib/npg_qc/autoqc/results/genotype_call.pm lib/npg_qc/autoqc/results/insert_size.pm -lib/npg_qc/autoqc/results/meta.pm lib/npg_qc/autoqc/results/pulldown_metrics.pm lib/npg_qc/autoqc/results/qX_yield.pm lib/npg_qc/autoqc/results/ref_match.pm lib/npg_qc/autoqc/results/result.pm +lib/npg_qc/autoqc/results/review.pm lib/npg_qc/autoqc/results/rna_seqc.pm lib/npg_qc/autoqc/results/samtools_stats.pm lib/npg_qc/autoqc/results/sequence_error.pm @@ -206,10 +206,10 @@ t/60-autoqc-checks-gc_fraction.t t/60-autoqc-checks-genotype.t t/60-autoqc-checks-genotype_call.t t/60-autoqc-checks-insert_size.t -t/60-autoqc-checks-meta.t t/60-autoqc-checks-pulldown_metrics.t t/60-autoqc-checks-qX_yield.t t/60-autoqc-checks-ref_match.t +t/60-autoqc-checks-review.t t/60-autoqc-checks-rna_seqc.t t/60-autoqc-checks-sequence_error.t t/60-autoqc-checks-spatial_filter.t @@ -235,11 +235,11 @@ t/60-autoqc-results-gc_fraction.t t/60-autoqc-results-genotype.t t/60-autoqc-results-genotype_call.t t/60-autoqc-results-insert_size.t -t/60-autoqc-results-meta.t t/60-autoqc-results-pulldown_metrics.t t/60-autoqc-results-qX_yield.t t/60-autoqc-results-ref_match.t t/60-autoqc-results-result.t +t/60-autoqc-results-review.t t/60-autoqc-results-rna_seqc.t t/60-autoqc-results-samtools_stats.t t/60-autoqc-results-sequence_error.t @@ -422,18 +422,6 @@ t/data/autoqc/load/3510_1_1_human_split_stats.json t/data/autoqc/load/3510_5.qX_yield.json t/data/autoqc/load/do_not_add_files_to_this_dir t/data/autoqc/load/some.json -t/data/autoqc/meta/product_release.yml -t/data/autoqc/meta/samplesheet_27483.csv -t/data/autoqc/meta/samplesheet_29524.csv -t/data/autoqc/meta/29524#2.composition.json -t/data/autoqc/meta/29524#17.bam_flagstats.json -t/data/autoqc/meta/29524#2.bam_flagstats.json -t/data/autoqc/meta/29524#2.bcfstats.json -t/data/autoqc/meta/29524#2.qX_yield.json -t/data/autoqc/meta/29524#2.verify_bam_id.json -t/data/autoqc/meta/29524#2_phix.bam_flagstats.json -t/data/autoqc/meta/29524#3.bam_flagstats.json -t/data/autoqc/meta/29524#7.bam_flagstats.json t/data/autoqc/npg/instrument/15.xml t/data/autoqc/npg/instrument/42.xml t/data/autoqc/npg/run/1937.xml @@ -486,6 +474,18 @@ t/data/autoqc/rendered/json_single_run/3612_7.insert_size.json t/data/autoqc/rendered/json_single_run/3612_7.qX_yield.json t/data/autoqc/rendered/json_single_run/3612_8.insert_size.json t/data/autoqc/rendered/json_single_run/3612_8.qX_yield.json +t/data/autoqc/review/product_release.yml +t/data/autoqc/review/samplesheet_27483.csv +t/data/autoqc/review/samplesheet_29524.csv +t/data/autoqc/review/29524#2.composition.json +t/data/autoqc/review/29524#17.bam_flagstats.json +t/data/autoqc/review/29524#2.bam_flagstats.json +t/data/autoqc/review/29524#2.bcfstats.json +t/data/autoqc/review/29524#2.qX_yield.json +t/data/autoqc/review/29524#2.verify_bam_id.json +t/data/autoqc/review/29524#2_phix.bam_flagstats.json +t/data/autoqc/review/29524#3.bam_flagstats.json +t/data/autoqc/review/29524#7.bam_flagstats.json t/data/autoqc/rna_seqc/data/17550_1#1.bam t/data/autoqc/rna_seqc/data/17550_3#8.bam t/data/autoqc/rna_seqc/samplesheet_17550.csv diff --git a/lib/npg_qc/autoqc/checks/meta.pm b/lib/npg_qc/autoqc/checks/review.pm similarity index 99% rename from lib/npg_qc/autoqc/checks/meta.pm rename to lib/npg_qc/autoqc/checks/review.pm index c184e25e7..6a12dae15 100644 --- a/lib/npg_qc/autoqc/checks/meta.pm +++ b/lib/npg_qc/autoqc/checks/review.pm @@ -1,4 +1,4 @@ -package npg_qc::autoqc::checks::meta; +package npg_qc::autoqc::checks::review; use Moose; use namespace::autoclean; @@ -31,11 +31,11 @@ Readonly::Scalar my $TIMESTAMP_FORMAT_WOFFSET => q[%Y-%m-%dT%T%z]; =head1 NAME -npg_qc::autoqc::checks::meta +npg_qc::autoqc::checks::review =head1 SYNOPSIS - my $check = npg_qc::autoqc::checks::meta->new(qc_in => 'dir_in'); + my $check = npg_qc::autoqc::checks::review->new(qc_in => 'dir_in'); $check->execute(); =head1 DESCRIPTION diff --git a/lib/npg_qc/autoqc/results/meta.pm b/lib/npg_qc/autoqc/results/review.pm similarity index 96% rename from lib/npg_qc/autoqc/results/meta.pm rename to lib/npg_qc/autoqc/results/review.pm index aa8d466ee..c05988f29 100644 --- a/lib/npg_qc/autoqc/results/meta.pm +++ b/lib/npg_qc/autoqc/results/review.pm @@ -1,4 +1,4 @@ -package npg_qc::autoqc::results::meta; +package npg_qc::autoqc::results::review; use Moose; use namespace::autoclean; @@ -12,7 +12,7 @@ our $VERSION = '0'; =head1 NAME -npg_qc::autoqc::results::meta +npg_qc::autoqc::results::review =head1 SYNOPSIS diff --git a/t/60-autoqc-checks-meta.t b/t/60-autoqc-checks-review.t similarity index 89% rename from t/60-autoqc-checks-meta.t rename to t/60-autoqc-checks-review.t index 8caaf7d2d..25f36d425 100644 --- a/t/60-autoqc-checks-meta.t +++ b/t/60-autoqc-checks-review.t @@ -13,14 +13,14 @@ use npg_testing::db; use npg_qc::autoqc::qc_store; use npg_tracking::glossary::composition; -use_ok('npg_qc::autoqc::checks::meta'); +use_ok('npg_qc::autoqc::checks::review'); my $dir = tempdir( CLEANUP => 1 ); -my $test_data_dir = 't/data/autoqc/meta'; +my $test_data_dir = 't/data/autoqc/review'; my $conf_file_path = "$test_data_dir/product_release.yml"; local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = - 't/data/autoqc/meta/samplesheet_27483.csv'; + 't/data/autoqc/review/samplesheet_27483.csv'; my $criteria_list = [ '( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95', @@ -33,12 +33,12 @@ my $criteria_list = [ subtest 'construction object, deciding whether to run' => sub { plan tests => 19; - my $check = npg_qc::autoqc::checks::meta->new( + my $check = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $test_data_dir, rpt_list => '27483:1:2'); - isa_ok ($check, 'npg_qc::autoqc::checks::meta'); - isa_ok ($check->result, 'npg_qc::autoqc::results::meta'); + isa_ok ($check, 'npg_qc::autoqc::checks::review'); + isa_ok ($check->result, 'npg_qc::autoqc::results::review'); my $can_run; warnings_like { $can_run = $check->can_run } [qr/Reading product configuration from/, @@ -52,7 +52,7 @@ subtest 'construction object, deciding whether to run' => sub { is ($check->result->pass, undef, 'pass atttribute of the result object is ndefined'); - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/no_robo", qc_in => $test_data_dir, rpt_list => '27483:1:2'); @@ -65,7 +65,7 @@ subtest 'construction object, deciding whether to run' => sub { 'No criteria defined in the product configuration file', 'reason logged'); - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/no_criteria", qc_in => $test_data_dir, rpt_list => '27483:1:2'); @@ -78,7 +78,7 @@ subtest 'construction object, deciding whether to run' => sub { 'No criteria defined in the product configuration file', 'reason logged'); - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/with_criteria", qc_in => $test_data_dir, rpt_list => '27483:1:2'); @@ -89,13 +89,13 @@ subtest 'construction object, deciding whether to run' => sub { ok (!$check->result->comments, 'No comments logged'); is_deeply ($check->_criteria, {'and' => $criteria_list}, 'criteria parsed correctly'); - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/error1", qc_in => $test_data_dir, rpt_list => '27483:1:2'); throws_ok { $check->can_run } qr/acceptance_criteria key is missing/, 'conf file format is incorrect'; - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/error2", qc_in => $test_data_dir, rpt_list => '27483:1:2'); @@ -106,7 +106,7 @@ subtest 'construction object, deciding whether to run' => sub { subtest 'setting options for qc store' => sub { plan tests => 4; - my $check = npg_qc::autoqc::checks::meta->new( + my $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/with_criteria", qc_in => $test_data_dir, rpt_list => '27483:1:2'); @@ -126,7 +126,7 @@ subtest 'finding result - file system' => sub { my $expected = 'Expected results for bam_flagstats, bcfstats, verify_bam_id,'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; - my $check = npg_qc::autoqc::checks::meta->new( + my $check = npg_qc::autoqc::checks::review->new( conf_path => "$test_data_dir/with_criteria", qc_in => $test_data_dir, rpt_list => '27483:1:2'); @@ -136,13 +136,13 @@ subtest 'finding result - file system' => sub { # and gradually add them to qc_in local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = - 't/data/autoqc/meta/samplesheet_29524.csv'; + 't/data/autoqc/review/samplesheet_29524.csv'; for my $name (('29524#2.qX_yield.json', '29524#2_phix.bam_flagstats.json', '29524#7.bam_flagstats.json')) { copy "$test_data_dir/$name", "$dir/$name"; - my $c = npg_qc::autoqc::checks::meta->new( + my $c = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $dir, rpt_list => $rpt_list); @@ -151,7 +151,7 @@ subtest 'finding result - file system' => sub { my $name = '29524#2.bam_flagstats.json'; copy "$test_data_dir/$name", "$dir/$name"; - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $dir, rpt_list => $rpt_list); @@ -160,7 +160,7 @@ subtest 'finding result - file system' => sub { $name = '29524#2.verify_bam_id.json'; copy "$test_data_dir/$name", "$dir/$name"; - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $dir, rpt_list => '29524:1:2;29524:2:2;29524:3:2;29524:4:2'); @@ -170,7 +170,7 @@ subtest 'finding result - file system' => sub { $name = '29524#2.bcfstats.json'; copy "$test_data_dir/$name", "$dir/$name"; - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $test_data_dir, rpt_list => $rpt_list); @@ -191,7 +191,7 @@ subtest 'finding results - database' => sub { plan tests => 15; local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = - 't/data/autoqc/meta/samplesheet_29524.csv'; + 't/data/autoqc/review/samplesheet_29524.csv'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; @@ -209,7 +209,7 @@ subtest 'finding results - database' => sub { ) }; my $check; - lives_ok { $check = npg_qc::autoqc::checks::meta->new($init) } + lives_ok { $check = npg_qc::autoqc::checks::review->new($init) } 'object created without qc_in defined'; my $expected = 'Expected results for bam_flagstats, bcfstats, verify_bam_id,'; @@ -220,7 +220,7 @@ subtest 'finding results - database' => sub { read_file "$test_data_dir/29524#2.composition.json"); my $row = $schema->resultset('BamFlagstats') ->find_or_create_seq_composition($composition); - $check = npg_qc::autoqc::checks::meta->new($init); + $check = npg_qc::autoqc::checks::review->new($init); throws_ok { $check->_results } qr/$expected found none/, 'no results - error'; my $create_qc_record = sub { @@ -238,7 +238,7 @@ subtest 'finding results - database' => sub { $init->{'qc_in'} = $test_data_dir; - $check = npg_qc::autoqc::checks::meta->new($init); + $check = npg_qc::autoqc::checks::review->new($init); throws_ok { $check->_results } qr/$expected found results for bam_flagstats/, 'not all results - error'; @@ -248,7 +248,7 @@ subtest 'finding results - database' => sub { $create_qc_record->('29524#2.verify_bam_id.json', 'VerifyBamId'); - $check = npg_qc::autoqc::checks::meta->new($init); + $check = npg_qc::autoqc::checks::review->new($init); lives_ok { $check->_results } 'no error - all expected results loaded'; is_deeply ([sort keys %{$check->_results}], [sort qw/bam_flagstats bcfstats verify_bam_id/], 'correct keys'); @@ -264,7 +264,7 @@ subtest 'single expression evaluation' => sub { plan tests => 10; local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = - 't/data/autoqc/meta/samplesheet_29524.csv'; + 't/data/autoqc/review/samplesheet_29524.csv'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; my $criteria = [ @@ -275,7 +275,7 @@ subtest 'single expression evaluation' => sub { '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02' ]; - my $check = npg_qc::autoqc::checks::meta->new( + my $check = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $test_data_dir, rpt_list => $rpt_list); @@ -304,10 +304,10 @@ subtest 'evaluation within the execute method' => sub { plan tests => 24; local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = - 't/data/autoqc/meta/samplesheet_29524.csv'; + 't/data/autoqc/review/samplesheet_29524.csv'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; - my $check = npg_qc::autoqc::checks::meta->new( + my $check = npg_qc::autoqc::checks::review->new( conf_path => $test_data_dir, qc_in => $dir, rpt_list => $rpt_list); @@ -338,7 +338,7 @@ subtest 'evaluation within the execute method' => sub { } write_file($target, to_json($values)); - $check = npg_qc::autoqc::checks::meta->new( + $check = npg_qc::autoqc::checks::review->new( final_qc_outcome => 1, conf_path => $test_data_dir, qc_in => $dir, diff --git a/t/60-autoqc-results-collection.t b/t/60-autoqc-results-collection.t index 0623d9a7d..d392125bd 100644 --- a/t/60-autoqc-results-collection.t +++ b/t/60-autoqc-results-collection.t @@ -39,6 +39,7 @@ my $temp = tempdir( CLEANUP => 1); tag_decode_stats => 1, bam_flagstats => 1, ref_match => 1, + review => 1, tag_metrics => 1, pulldown_metrics => 1, alignment_filter_metrics => 1, diff --git a/t/60-autoqc-results-meta.t b/t/60-autoqc-results-review.t similarity index 83% rename from t/60-autoqc-results-meta.t rename to t/60-autoqc-results-review.t index 1e0e68ebf..72aecce11 100644 --- a/t/60-autoqc-results-meta.t +++ b/t/60-autoqc-results-review.t @@ -6,7 +6,7 @@ use Test::Exception; use npg_tracking::glossary::composition::component::illumina; use npg_tracking::glossary::composition; -use_ok('npg_qc::autoqc::results::meta'); +use_ok('npg_qc::autoqc::results::review'); my $composition = npg_tracking::glossary::composition->new( components => [ @@ -19,8 +19,8 @@ my $composition = npg_tracking::glossary::composition->new( ] ); -my $r = npg_qc::autoqc::results::meta->new(composition => $composition); -isa_ok ($r, 'npg_qc::autoqc::results::meta'); +my $r = npg_qc::autoqc::results::review->new(composition => $composition); +isa_ok ($r, 'npg_qc::autoqc::results::review'); $r->qc_outcome({mqc_outcome => 'Accepted_final', timestamp => '2019-05-23T17:11:31+0100', username => 'robo_qc'}); diff --git a/t/data/autoqc/meta/29524#17.bam_flagstats.json b/t/data/autoqc/review/29524#17.bam_flagstats.json similarity index 100% rename from t/data/autoqc/meta/29524#17.bam_flagstats.json rename to t/data/autoqc/review/29524#17.bam_flagstats.json diff --git a/t/data/autoqc/meta/29524#2.bam_flagstats.json b/t/data/autoqc/review/29524#2.bam_flagstats.json similarity index 100% rename from t/data/autoqc/meta/29524#2.bam_flagstats.json rename to t/data/autoqc/review/29524#2.bam_flagstats.json diff --git a/t/data/autoqc/meta/29524#2.bcfstats.json b/t/data/autoqc/review/29524#2.bcfstats.json similarity index 100% rename from t/data/autoqc/meta/29524#2.bcfstats.json rename to t/data/autoqc/review/29524#2.bcfstats.json diff --git a/t/data/autoqc/meta/29524#2.composition.json b/t/data/autoqc/review/29524#2.composition.json similarity index 100% rename from t/data/autoqc/meta/29524#2.composition.json rename to t/data/autoqc/review/29524#2.composition.json diff --git a/t/data/autoqc/meta/29524#2.qX_yield.json b/t/data/autoqc/review/29524#2.qX_yield.json similarity index 100% rename from t/data/autoqc/meta/29524#2.qX_yield.json rename to t/data/autoqc/review/29524#2.qX_yield.json diff --git a/t/data/autoqc/meta/29524#2.verify_bam_id.json b/t/data/autoqc/review/29524#2.verify_bam_id.json similarity index 100% rename from t/data/autoqc/meta/29524#2.verify_bam_id.json rename to t/data/autoqc/review/29524#2.verify_bam_id.json diff --git a/t/data/autoqc/meta/29524#2_phix.bam_flagstats.json b/t/data/autoqc/review/29524#2_phix.bam_flagstats.json similarity index 100% rename from t/data/autoqc/meta/29524#2_phix.bam_flagstats.json rename to t/data/autoqc/review/29524#2_phix.bam_flagstats.json diff --git a/t/data/autoqc/meta/29524#3.bam_flagstats.json b/t/data/autoqc/review/29524#3.bam_flagstats.json similarity index 100% rename from t/data/autoqc/meta/29524#3.bam_flagstats.json rename to t/data/autoqc/review/29524#3.bam_flagstats.json diff --git a/t/data/autoqc/meta/29524#7.bam_flagstats.json b/t/data/autoqc/review/29524#7.bam_flagstats.json similarity index 100% rename from t/data/autoqc/meta/29524#7.bam_flagstats.json rename to t/data/autoqc/review/29524#7.bam_flagstats.json diff --git a/t/data/autoqc/meta/error1/product_release.yml b/t/data/autoqc/review/error1/product_release.yml similarity index 100% rename from t/data/autoqc/meta/error1/product_release.yml rename to t/data/autoqc/review/error1/product_release.yml diff --git a/t/data/autoqc/meta/error2/product_release.yml b/t/data/autoqc/review/error2/product_release.yml similarity index 100% rename from t/data/autoqc/meta/error2/product_release.yml rename to t/data/autoqc/review/error2/product_release.yml diff --git a/t/data/autoqc/meta/no_criteria/product_release.yml b/t/data/autoqc/review/no_criteria/product_release.yml similarity index 100% rename from t/data/autoqc/meta/no_criteria/product_release.yml rename to t/data/autoqc/review/no_criteria/product_release.yml diff --git a/t/data/autoqc/meta/no_robo/product_release.yml b/t/data/autoqc/review/no_robo/product_release.yml similarity index 100% rename from t/data/autoqc/meta/no_robo/product_release.yml rename to t/data/autoqc/review/no_robo/product_release.yml diff --git a/t/data/autoqc/meta/product_release.yml b/t/data/autoqc/review/product_release.yml similarity index 100% rename from t/data/autoqc/meta/product_release.yml rename to t/data/autoqc/review/product_release.yml diff --git a/t/data/autoqc/meta/samplesheet_27483.csv b/t/data/autoqc/review/samplesheet_27483.csv similarity index 100% rename from t/data/autoqc/meta/samplesheet_27483.csv rename to t/data/autoqc/review/samplesheet_27483.csv diff --git a/t/data/autoqc/meta/samplesheet_29524.csv b/t/data/autoqc/review/samplesheet_29524.csv similarity index 100% rename from t/data/autoqc/meta/samplesheet_29524.csv rename to t/data/autoqc/review/samplesheet_29524.csv diff --git a/t/data/autoqc/meta/with_criteria/product_release.yml b/t/data/autoqc/review/with_criteria/product_release.yml similarity index 100% rename from t/data/autoqc/meta/with_criteria/product_release.yml rename to t/data/autoqc/review/with_criteria/product_release.yml From a93374601b0b4545d4450333f52cd8e63b4052b3 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Mon, 3 Jun 2019 15:40:35 +0100 Subject: [PATCH 15/23] avoid failure when retrieving db results if a result does not have a corresponding table --- lib/npg_qc/autoqc/qc_store.pm | 46 ++++++++++++++++++++++++++++------- 1 file changed, 37 insertions(+), 9 deletions(-) diff --git a/lib/npg_qc/autoqc/qc_store.pm b/lib/npg_qc/autoqc/qc_store.pm index c16fb0385..892ce7b21 100644 --- a/lib/npg_qc/autoqc/qc_store.pm +++ b/lib/npg_qc/autoqc/qc_store.pm @@ -344,15 +344,27 @@ sub load_from_db { if (!$table_class) { croak qq[No DBIx result class name for $check_name]; } - if ($query->option == $LANES) { - $dbix_query->{$ti_key} = undef; - } elsif ($query->option == $PLEXES) { - $dbix_query->{$ti_key} = {q[!=], undef}; - } - my @check_results = $self->qc_schema()->resultset($table_class) - ->search_autoqc($dbix_query)->all(); - push @results, @check_results; + my $rs; + ##### + # A database table for the result does not have to exist. + # The try statement below takes care of this. + try { + $rs = $self->qc_schema()->resultset($table_class); + }; + + if ($rs) { + + if ($query->option == $LANES) { + $dbix_query->{$ti_key} = undef; + } elsif ($query->option == $PLEXES) { + $dbix_query->{$ti_key} = {q[!=], undef}; + } + + my @check_results = $self->qc_schema()->resultset($table_class) + ->search_autoqc($dbix_query)->all(); + push @results, @check_results; + } } } else { carp __PACKAGE__ . q[ object is configured not to use the database]; @@ -399,8 +411,24 @@ sub load_from_db_via_composition { {'me.digest' => [map { $_->digest } @{$compositions}]}, {join => $self->_relation_names} ); + + my @relation_names = @{$self->_relation_names}; while (my $row = $rs->next()) { - push @results, grep { $_ } map { $row->$_ } @{$self->_relation_names}; + foreach my $rname (@relation_names) { + my $rrow; + ##### + # A database table for the result does not have to exist. + # The try statement below takes care of this. The error might be due + # to the relation name being different from what we think it is. + # So, if results from a table are nevr retrieved, check the relation + # name. + try { + $rrow = $row->$rname; + }; + if ($rrow) { + push @results, $rrow; + } + } } } my $c = npg_qc::autoqc::results::collection->new(); From 1e136086d53a1b3da07414e8d7bad0c6c38fcdee Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Mon, 3 Jun 2019 16:47:49 +0100 Subject: [PATCH 16/23] use test iRODS server --- t/60-autoqc-checks-genotype.t | 120 ++++++++++++++++++++-------------- 1 file changed, 71 insertions(+), 49 deletions(-) diff --git a/t/60-autoqc-checks-genotype.t b/t/60-autoqc-checks-genotype.t index 61b53001d..123cac606 100644 --- a/t/60-autoqc-checks-genotype.t +++ b/t/60-autoqc-checks-genotype.t @@ -8,67 +8,89 @@ use WTSI::NPG::iRODS; use_ok ('npg_qc::autoqc::checks::genotype'); -my $ref_repos = cwd . '/t/data/autoqc'; -my $expected_md5 = q[a4790111996a3f1c0247d65f4998e492]; - my $dir = tempdir(CLEANUP => 1); -my $st = join q[/], $dir, q[samtools]; -`touch $st`; -`chmod +x $st`; -my $bt = join q[/], $dir, q[bcftools]; -`touch $bt`; -`chmod +x $bt`; -local $ENV{PATH} = join q[:], $dir, $ENV{PATH}; -my $data_dir = $dir."/data"; -mkdir($data_dir); -`cp t/data/autoqc/alignment.bam $data_dir/2_1.bam`; -`echo -n $expected_md5 > $data_dir/2_1.bam.md5`; +my $pid = $$; +my $collection_name = "GenotypeTest.$pid"; + +my $have_irods_execs = exist_irods_executables(); +my $env_file = $ENV{'WTSI_NPG_iRODS_Test_IRODS_ENVIRONMENT_FILE'} || q[]; +local $ENV{'IRODS_ENVIRONMENT_FILE'} = $env_file || 'DUMMY_VALUE'; -# create and populate a temporary iRODS collection -my $irods = WTSI::NPG::iRODS->new(strict_baton_version => 0); my $irods_tmp_coll; -my $irods_data_coll; -my $pid = $$; -$irods_tmp_coll = $irods->add_collection("GenotypeTest.$pid"); -$irods->put_collection($data_dir, $irods_tmp_coll); -$irods_data_coll = $irods_tmp_coll."/data"; +my $irods; +if ($env_file && $have_irods_execs) { + $irods = WTSI::NPG::iRODS->new(strict_baton_version => 0); + $irods_tmp_coll = $irods->add_collection("GenotypeTest.$pid") ; +} + +sub exist_irods_executables { + return 0 unless `which ienv`; + return 0 unless `which imkdir`; + return 1; +} + +END { + # remove temporary iRODS collection + if($irods_tmp_coll) { + local $ENV{'IRODS_ENVIRONMENT_FILE'} = $env_file; + eval {system("irm -r $collection_name")}; + } +} -{ - my $r = npg_qc::autoqc::checks::genotype->new( +SKIP: { + skip 'iRODS not available', 9 unless $irods_tmp_coll; + + my $ref_repos = cwd . '/t/data/autoqc'; + my $expected_md5 = q[a4790111996a3f1c0247d65f4998e492]; + + my $st = join q[/], $dir, q[samtools]; + `touch $st`; + `chmod +x $st`; + my $bt = join q[/], $dir, q[bcftools]; + `touch $bt`; + `chmod +x $bt`; + local $ENV{PATH} = join q[:], $dir, $ENV{PATH}; + my $data_dir = $dir."/data"; + mkdir($data_dir); + `cp t/data/autoqc/alignment.bam $data_dir/2_1.bam`; + `echo -n $expected_md5 > $data_dir/2_1.bam.md5`; + + # populate a temporary iRODS collection + $irods->put_collection($data_dir, $irods_tmp_coll); + my $irods_data_coll = $irods_tmp_coll."/data"; + + my $r = npg_qc::autoqc::checks::genotype->new( irods => $irods, id_run => 2, position => 1, input_files => ["$data_dir/2_1.bam"], repository => $ref_repos, - ); - isa_ok ($r, 'npg_qc::autoqc::checks::genotype'); - lives_ok { $r->result; } 'No error creating result object'; - lives_ok {$r->samtools } 'No error calling "samtools" accessor'; - is($r->samtools, $st, 'correct samtools path'); - lives_ok {$r->bcftools } 'No error calling "bcftools" accessor'; - is($r->bcftools, $bt, 'correct bcftools path'); - is($r->input_files_md5, $expected_md5, - "Local MD5 string matches expected value"); + ); + isa_ok ($r, 'npg_qc::autoqc::checks::genotype'); + lives_ok { $r->result; } 'No error creating result object'; + lives_ok {$r->samtools } 'No error calling "samtools" accessor'; + is($r->samtools, $st, 'correct samtools path'); + lives_ok {$r->bcftools } 'No error calling "bcftools" accessor'; + is($r->bcftools, $bt, 'correct bcftools path'); + is($r->input_files_md5, $expected_md5, + "Local MD5 string matches expected value"); - lives_ok {npg_qc::autoqc::checks::genotype->new( - repository => $ref_repos, rpt_list => '2:1', path => q[t]); } - 'object via the rpt_list'; + lives_ok {npg_qc::autoqc::checks::genotype->new( + repository => $ref_repos, rpt_list => '2:1', path => q[t]); } + 'object via the rpt_list'; - my $irods_path = 'irods:'.$irods_data_coll.'/2_1.bam'; - $r = npg_qc::autoqc::checks::genotype->new( - irods => $irods, - id_run => 2, - position => 1, - input_files => [$irods_path], - repository => $ref_repos, - ); - is($r->input_files_md5, $expected_md5, - "iRODS MD5 string matches expected value") or - diag `ils -L $irods_data_coll/2_1.bam` + my $irods_path = 'irods:'.$irods_data_coll.'/2_1.bam'; + $r = npg_qc::autoqc::checks::genotype->new( + irods => $irods, + id_run => 2, + position => 1, + input_files => [$irods_path], + repository => $ref_repos, + ); + is($r->input_files_md5, $expected_md5, + "iRODS MD5 string matches expected value") or + diag `ils -L $irods_data_coll/2_1.bam` } -# remove temporary iRODS collection -$irods->remove_collection($irods_tmp_coll); - 1; From 3fb7dc73d70c92bb6fa19e1acbeb873dd9187ba2 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Tue, 4 Jun 2019 08:54:14 +0100 Subject: [PATCH 17/23] review results should not be displayed along other results --- npg_qc_viewer/root/src/ui_lanes/collapse_menu.tt2 | 2 +- npg_qc_viewer/root/src/ui_lanes/library_lanes.tt2 | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/npg_qc_viewer/root/src/ui_lanes/collapse_menu.tt2 b/npg_qc_viewer/root/src/ui_lanes/collapse_menu.tt2 index 0905224a2..86976960c 100644 --- a/npg_qc_viewer/root/src/ui_lanes/collapse_menu.tt2 +++ b/npg_qc_viewer/root/src/ui_lanes/collapse_menu.tt2 @@ -5,7 +5,7 @@ + all results [%- FOREACH check_name IN checks_info.list -%] - [%- IF (check_name != "spatial filter") %] + [%- IF (check_name != "spatial filter") && (check_name != "review")%] [%- label_name = check_name; IF check_name == "sequence mismatch"; label_name='seq. mismatch'; ELSIF check_name == "alignment filter metrics"; label_name='alignment filter'; diff --git a/npg_qc_viewer/root/src/ui_lanes/library_lanes.tt2 b/npg_qc_viewer/root/src/ui_lanes/library_lanes.tt2 index 5585d8cca..70ce303c7 100644 --- a/npg_qc_viewer/root/src/ui_lanes/library_lanes.tt2 +++ b/npg_qc_viewer/root/src/ui_lanes/library_lanes.tt2 @@ -8,7 +8,7 @@ tag_metrics_cache = {}; temp = []; FOREACH check_name IN checks_info.list; - IF (check_name != 'samtools stats') && (check_name != 'bam flagstats human') && (check_name != 'bam flagstats phix') && (check_name != 'gc bias'); + IF (check_name != 'review') && (check_name != 'samtools stats') && (check_name != 'bam flagstats human') && (check_name != 'bam flagstats phix') && (check_name != 'gc bias'); IF check_name == 'tag metrics'; temp.unshift(check_name); ELSE; From df9beb2419c812a7d48423289bdbbd745c071d95 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Tue, 4 Jun 2019 08:55:36 +0100 Subject: [PATCH 18/23] visual que for failed autoqc review results --- npg_qc_viewer/root/src/ui_lanes/lane.tt2 | 6 ++++++ npg_qc_viewer/root/static/stylesheets/npg_seqqc.css | 4 ++++ 2 files changed, 10 insertions(+) diff --git a/npg_qc_viewer/root/src/ui_lanes/lane.tt2 b/npg_qc_viewer/root/src/ui_lanes/lane.tt2 index 1ff2f96e8..2cea64a73 100644 --- a/npg_qc_viewer/root/src/ui_lanes/lane.tt2 +++ b/npg_qc_viewer/root/src/ui_lanes/lane.tt2 @@ -3,6 +3,12 @@ lane_class = 'lane'; plex_class = 'tag_info'; + IF lane_collection && lane_collection.size; + review_obj = lane_collection.slice('check_name', 'review').get(0); + IF review_obj && review_obj.pass.defined && review_obj.pass == 0; + plex_class = plex_class _ ' failed_review'; + END; + END; -%] diff --git a/npg_qc_viewer/root/static/stylesheets/npg_seqqc.css b/npg_qc_viewer/root/static/stylesheets/npg_seqqc.css index e7bb4f114..b5f8a8350 100644 --- a/npg_qc_viewer/root/static/stylesheets/npg_seqqc.css +++ b/npg_qc_viewer/root/static/stylesheets/npg_seqqc.css @@ -269,6 +269,10 @@ a, a:link, a:visited, a:hover { background-color: #f99389; } +.failed_review { + border: 3px solid #f99389; +} + /* Used to format error messages from mqc. */ .failed_mqc { background-color: #FFFFFF; From 92a5c148d62542a16b9c0e2218e623809e60a3de Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Thu, 6 Jun 2019 13:09:16 +0100 Subject: [PATCH 19/23] ensure tests are using LIMs data frim a samplesheet --- t/60-autoqc-checks-review.t | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/t/60-autoqc-checks-review.t b/t/60-autoqc-checks-review.t index 25f36d425..4bbc40c47 100644 --- a/t/60-autoqc-checks-review.t +++ b/t/60-autoqc-checks-review.t @@ -19,7 +19,7 @@ my $dir = tempdir( CLEANUP => 1 ); my $test_data_dir = 't/data/autoqc/review'; my $conf_file_path = "$test_data_dir/product_release.yml"; -local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = +local $ENV{NPG_CACHED_SAMPLESHEET_FILE} = 't/data/autoqc/review/samplesheet_27483.csv'; my $criteria_list = [ @@ -135,7 +135,7 @@ subtest 'finding result - file system' => sub { # should have all three for the entiry and phix for bam_flagstats and qx_yield # and gradually add them to qc_in - local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + local $ENV{NPG_CACHED_SAMPLESHEET_FILE} = 't/data/autoqc/review/samplesheet_29524.csv'; for my $name (('29524#2.qX_yield.json', @@ -190,7 +190,7 @@ subtest 'finding result - file system' => sub { subtest 'finding results - database' => sub { plan tests => 15; - local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + local $ENV{NPG_CACHED_SAMPLESHEET_FILE} = 't/data/autoqc/review/samplesheet_29524.csv'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; @@ -263,7 +263,7 @@ subtest 'finding results - database' => sub { subtest 'single expression evaluation' => sub { plan tests => 10; - local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + local $ENV{NPG_CACHED_SAMPLESHEET_FILE} = 't/data/autoqc/review/samplesheet_29524.csv'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; @@ -303,7 +303,7 @@ subtest 'single expression evaluation' => sub { subtest 'evaluation within the execute method' => sub { plan tests => 24; - local $ENV{NPG_CACHED_SAMPLSHEET_FILE} = + local $ENV{NPG_CACHED_SAMPLESHEET_FILE} = 't/data/autoqc/review/samplesheet_29524.csv'; my $rpt_list = '29524:1:2;29524:2:2;29524:3:2;29524:4:2'; From 2a1dc644520ff0c87925bceb0461ce0f23c1416c Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Tue, 11 Jun 2019 11:39:35 +0100 Subject: [PATCH 20/23] allow 'last_modified' date in mqc tables to be pre-set --- lib/npg_qc/Schema/Mqc/OutcomeEntity.pm | 4 +++- t/50-schema-result-MqcLibraryOutcomeEnt.t | 15 +++++++++++++-- 2 files changed, 16 insertions(+), 3 deletions(-) diff --git a/lib/npg_qc/Schema/Mqc/OutcomeEntity.pm b/lib/npg_qc/Schema/Mqc/OutcomeEntity.pm index 50f6a2bf9..946637dbd 100644 --- a/lib/npg_qc/Schema/Mqc/OutcomeEntity.pm +++ b/lib/npg_qc/Schema/Mqc/OutcomeEntity.pm @@ -63,7 +63,9 @@ sub add_common_ent_methods { around [qw/update insert/] => sub { my $orig = shift; my $self = shift; - $self->last_modified($self->get_time_now); + if (!$self->is_column_changed('last_modified')) { + $self->last_modified($self->get_time_now); + } my $return_super = $self->$orig(@_); $self->_create_historic(); return $return_super; diff --git a/t/50-schema-result-MqcLibraryOutcomeEnt.t b/t/50-schema-result-MqcLibraryOutcomeEnt.t index 2848e3174..58063cb8b 100644 --- a/t/50-schema-result-MqcLibraryOutcomeEnt.t +++ b/t/50-schema-result-MqcLibraryOutcomeEnt.t @@ -3,6 +3,7 @@ use warnings; use Test::More tests => 9; use Test::Exception; use Moose::Meta::Class; +use DateTime; use npg_testing::db; use t::autoqc_util; @@ -162,7 +163,7 @@ subtest 'insert with historic' => sub { }; subtest q[update] => sub { - plan tests => 51; + plan tests => 55; my $rs = $schema->resultset($table); my $hrs = $schema->resultset($hist_table); @@ -236,12 +237,22 @@ subtest q[update] => sub { 'final outcome saved'; ok ($new_row->in_storage, 'outcome has been saved'); is ($new_row->mqc_outcome->short_desc, $outcome, 'final outcome'); + my $dt = DateTime->now(); + is ($new_row->last_modified()->year, $dt->year, 'the "now" year'); $outcome = 'Rejected final'; - lives_ok { $new_row->update_outcome(_outcome($outcome), 'cat') } + + my $h = _outcome($outcome); + # pre-set the date + $h->{'last_modified'} = DateTime->new(year => 2016, month => 10, day => 25); + lives_ok { $new_row->update_outcome($h, 'cat') } 'can update final outcome'; ok ($new_row->in_storage, 'outcome has been saved'); is ($new_row->mqc_outcome->short_desc, $outcome, 'new final outcome'); + $dt = $new_row->last_modified(); + is ($dt->year, 2016, 'year as set, no overwrite by the current time'); + is ($dt->month, 10, 'month as set'); + is ($dt->day, 25, 'day as set'); $new_row->delete(); }; From a3fe9b0baa324f0e853d811f85eb93061e4926a3 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Tue, 11 Jun 2019 17:28:50 +0100 Subject: [PATCH 21/23] fix description generation for undecided preliminary outcome --- lib/npg_qc/Schema/Mqc/OutcomeDict.pm | 32 +++++++++++++++++----- t/50-schema-result-MqcLibraryOutcomeDict.t | 4 +-- 2 files changed, 27 insertions(+), 9 deletions(-) diff --git a/lib/npg_qc/Schema/Mqc/OutcomeDict.pm b/lib/npg_qc/Schema/Mqc/OutcomeDict.pm index 0973094f6..d66001892 100644 --- a/lib/npg_qc/Schema/Mqc/OutcomeDict.pm +++ b/lib/npg_qc/Schema/Mqc/OutcomeDict.pm @@ -69,7 +69,11 @@ sub generate_short_description { defined $is_final or croak 'Final flag should be defined'; my $decision = $is_accepted ? $ACCEPTED : (defined $is_accepted ? $REJECTED : $UNDECIDED); - return join q[ ], $decision, $is_final ? $FINAL : $PRELIMINARY; + if (defined $is_accepted || $is_final) { + $decision .= q[ ] . ($is_final ? $FINAL : $PRELIMINARY); + } + + return $decision; } no Moose::Role; @@ -149,14 +153,28 @@ __END__ =head2 generate_short_description Package-level method for generating descriptions matching short - descriptions in the dictionary tables. + descriptions in the dictionary tables. Note that not every description + is available in all dictionary tables. + + my $is_final = 1; + my $is_accepted = 1; + + __PACKAGE__->generate_short_description($is_final, $is_accepted); + # returns 'Accepted final' + + __PACKAGE__->generate_short_description($is_final); + # returns 'Undecided final' + + $is_accepted = 0; + __PACKAGE__->generate_short_description($is_final); + # returns 'Rejected final' + + $is_final = 0; + __PACKAGE__->generate_short_description($is_final, $is_accepted); + # returns 'Rejected preliminary' - my $is_final = 1; - my $is_accepted = 1; - __PACKAGE__->generate_short_description($is_final, $is_accepted); - # returns 'Accepted final' __PACKAGE__->generate_short_description($is_final); - # returns 'Undecided final' + # returns 'Undecided' !!! =head1 DIAGNOSTICS diff --git a/t/50-schema-result-MqcLibraryOutcomeDict.t b/t/50-schema-result-MqcLibraryOutcomeDict.t index ba5012a74..d63c7a0ef 100644 --- a/t/50-schema-result-MqcLibraryOutcomeDict.t +++ b/t/50-schema-result-MqcLibraryOutcomeDict.t @@ -60,9 +60,9 @@ is ($rows[0]->generate_short_description(0, 'accepted'), is ($rows[0]->generate_short_description(0, q[]), 'Rejected preliminary', 'description for prelim rejected'); is ($rows[0]->generate_short_description(0), - 'Undecided preliminary', 'description for prelim undecided'); + 'Undecided', 'description for prelim undecided'); is ($rows[0]->generate_short_description(0, undef), - 'Undecided preliminary', 'description for prelim undecided'); + 'Undecided', 'description for prelim undecided'); 1; From dbab7aab5c68cccd144bcca4fd7b7b9d3409837b Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Wed, 12 Jun 2019 13:41:30 +0100 Subject: [PATCH 22/23] ensure that the criteria can be compared --- lib/npg_qc/autoqc/checks/review.pm | 23 +++++++++++++++++++---- t/60-autoqc-checks-review.t | 4 ++-- 2 files changed, 21 insertions(+), 6 deletions(-) diff --git a/lib/npg_qc/autoqc/checks/review.pm b/lib/npg_qc/autoqc/checks/review.pm index 6a12dae15..d20d4462c 100644 --- a/lib/npg_qc/autoqc/checks/review.pm +++ b/lib/npg_qc/autoqc/checks/review.pm @@ -124,6 +124,15 @@ The outcome of the evaluation will be either true or false. The undecided outcome can be assigned if the overall expression evaluates to false and a full evaluation was performed (ie the acceptance branch was evaluated). +Since both the conjunction and disjunction operators are idempotent in +Boolean algebra, the order of expressions within the arrays does not +affect the outcome of evaluation. However, the order does matter when +comparing criteria either as data structures or serialized data +structures. To ensure that the criteria can be compared, the expressions +in the arrays are ordered alphabetically. Therefore, the criteria record +in the result object might vary slightly from the configuration file +record. + =head1 SUBROUTINES/METHODS =head2 use_db @@ -261,13 +270,15 @@ sub _build__criteria { if (keys %{$study_config}) { if ($study_config->{$ROBO_KEY}) { + my $lib_type = $self->_lims->library_type; $lib_type or croak "Library type is not defined for $description"; - $self->result->library_type($lib_type); # We are going to compare library type strings in lower case # because the case for this type of LIMs data might be inconsistent. my $original_lib_type = $lib_type; $lib_type = lc $lib_type; + # We will save the original library type. + $self->result->library_type($original_lib_type); ##### # We expect that the same criterium or a number of criteria can be @@ -294,6 +305,8 @@ sub _build__criteria { } } if (@criteria) { + # Sort to ensure a consistent order of expressions in the array. + @criteria = sort @criteria; # Applying the conjunction operator to all list members. $rewritten = {$CONJUNCTION_OP => \@criteria}; } else { @@ -400,11 +413,13 @@ sub _class_name_from_expression { return $class_name; } +##### +# Simplest traversal for now. Ideally, this function should take +# a callback so that different actions can be performed. +# sub _traverse { my ($node, $expressions) = @_; - # Simplest traversal for now. Ideally, this function should take - # a callback so that different actions can be performed. my $node_type = ref $node; if ($node_type) { ($node_type eq 'HASH') or croak "Unknown node type $node_type"; @@ -465,7 +480,7 @@ sub _evaluate_expression { my $class_name = _class_name_from_expression($e); my $obj = $self-> _results->{$class_name}; # We should not get this far with an error in the configuration - # filr, but just in case... + # file, but just in case... $obj or croak "No autoqc result for evaluation of '$e'"; ##no critic (ValuesAndExpressions::RequireInterpolationOfMetachars) diff --git a/t/60-autoqc-checks-review.t b/t/60-autoqc-checks-review.t index 4bbc40c47..6308e3ae4 100644 --- a/t/60-autoqc-checks-review.t +++ b/t/60-autoqc-checks-review.t @@ -24,10 +24,10 @@ local $ENV{NPG_CACHED_SAMPLESHEET_FILE} = my $criteria_list = [ '( bam_flagstats.target_proper_pair_mapped_reads / bam_flagstats.target_mapped_reads ) > 0.95', + '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02', 'bam_flagstats.target_mapped_bases > 85_000_000_000', 'bam_flagstats.target_percent_gt_coverage_threshold > 95', - 'verify_bam_id.freemix < 0.01', - '( bcfstats.genotypes_nrd_dividend / bcfstats.genotypes_nrd_divisor ) < 0.02' + 'verify_bam_id.freemix < 0.01' ]; subtest 'construction object, deciding whether to run' => sub { From 85e35d44af6acc8a2aa45cebd4d5ee2162554189 Mon Sep 17 00:00:00 2001 From: mgcam Date: Thu, 13 Jun 2019 14:13:32 +0100 Subject: [PATCH 23/23] Changes file update for release 66.5 --- Changes | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/Changes b/Changes index 484df3eee..3633b592d 100644 --- a/Changes +++ b/Changes @@ -1,8 +1,12 @@ LIST OF CHANGES FOR NPG-QC PACKAGE - +release 66.5 - update spatial_filter results parsing - add target autosome stats to bam_flagstats qc check + - allow 'last_modified' date in mqc tables to be pre-set + - new autoqc check 'review' to evaluate results of other + checks against configurable criteria + - SeqQC: add a visual que for failed autoqc review results release 66.4 - allow to finalise undecided library manual qc outcomes for

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