diff --git a/packages/web3-eth-accounts/CHANGELOG.md b/packages/web3-eth-accounts/CHANGELOG.md
index d9571feba9b..90a5cc590d4 100644
--- a/packages/web3-eth-accounts/CHANGELOG.md
+++ b/packages/web3-eth-accounts/CHANGELOG.md
@@ -107,3 +107,7 @@ Documentation:
 [Migration Guide from 1.x](https://docs.web3js.org/guides/web3_upgrade_guide/x/)
 
 ## [Unreleased]
+
+### Fixed
+
+-   Fixed "The `r` and `s` returned by `signTransaction` to does not always consist of 64 characters #6207" (#6216)
diff --git a/packages/web3-eth-accounts/src/account.ts b/packages/web3-eth-accounts/src/account.ts
index 7925033b736..64e18911901 100644
--- a/packages/web3-eth-accounts/src/account.ts
+++ b/packages/web3-eth-accounts/src/account.ts
@@ -271,8 +271,8 @@ export const signTransaction = async (
 	return {
 		messageHash: bytesToHex(signedTx.getMessageToSign(true)),
 		v: `0x${signedTx.v.toString(16)}`,
-		r: `0x${signedTx.r.toString(16)}`,
-		s: `0x${signedTx.s.toString(16)}`,
+		r: `0x${signedTx.r.toString(16).padStart(64, '0')}`,
+		s: `0x${signedTx.s.toString(16).padStart(64, '0')}`,
 		rawTransaction: rawTx,
 		transactionHash: bytesToHex(txHash),
 	};
diff --git a/packages/web3-eth-accounts/test/unit/account.test.ts b/packages/web3-eth-accounts/test/unit/account.test.ts
index 3bccc516b71..9b493fea298 100644
--- a/packages/web3-eth-accounts/test/unit/account.test.ts
+++ b/packages/web3-eth-accounts/test/unit/account.test.ts
@@ -104,9 +104,9 @@ describe('accounts', () => {
 			expect(signedResult.messageHash).toBeDefined();
 			expect(signedResult.rawTransaction).toBeDefined();
 			expect(signedResult.transactionHash).toBeDefined();
-			expect(signedResult.r).toBeDefined();
-			expect(signedResult.s).toBeDefined();
-			expect(signedResult.v).toBeDefined();
+			expect(signedResult.r).toMatch(/0[xX][0-9a-fA-F]{64}/);
+			expect(signedResult.s).toMatch(/0[xX][0-9a-fA-F]{64}/);
+			expect(signedResult.v).toMatch(/0[xX][0-9a-fA-F]+/);
 		});
 
 		it.each(transactionsTestData)('Recover transaction', async txData => {
diff --git a/packages/web3-eth/test/integration/web3_eth/sign_transaction.test.ts b/packages/web3-eth/test/integration/web3_eth/sign_transaction.test.ts
index 27be403c7ae..cf1f7452f77 100644
--- a/packages/web3-eth/test/integration/web3_eth/sign_transaction.test.ts
+++ b/packages/web3-eth/test/integration/web3_eth/sign_transaction.test.ts
@@ -61,8 +61,8 @@ describe('Web3Eth.signTransaction', () => {
 		// Pulling out of toMatchObject to be compatiable with Cypress
 		expect(response.raw).toMatch(/0[xX][0-9a-fA-F]+/);
 		expect(typeof (response.tx as TransactionLegacySignedAPI).v).toBe('bigint');
-		expect(response.tx.r).toMatch(/0[xX][0-9a-fA-F]+/);
-		expect(response.tx.s).toMatch(/0[xX][0-9a-fA-F]+/);
+		expect(response.tx.r).toMatch(/0[xX][0-9a-fA-F]{64}/);
+		expect(response.tx.s).toMatch(/0[xX][0-9a-fA-F]{64}/);
 	});
 
 	it('should sign a contract deployment', async () => {
@@ -90,7 +90,7 @@ describe('Web3Eth.signTransaction', () => {
 		// Pulling out of toMatchObject to be compatiable with Cypress
 		expect(response.raw).toMatch(/0[xX][0-9a-fA-F]+/);
 		expect(typeof (response.tx as TransactionLegacySignedAPI).v).toBe('bigint');
-		expect(response.tx.r).toMatch(/0[xX][0-9a-fA-F]+/);
-		expect(response.tx.s).toMatch(/0[xX][0-9a-fA-F]+/);
+		expect(response.tx.r).toMatch(/0[xX][0-9a-fA-F]{64}/);
+		expect(response.tx.s).toMatch(/0[xX][0-9a-fA-F]{64}/);
 	});
 });