- Fix segfault triggered by length-zero sequences
- Fix memory detection problem for some outdated MacOS versions
- Fix an incorrect assertion in unitig graph refreshing
- Added
--verbose
to output full log to the screen
- Add intermediate
megahit_core_popcnt
for CPUs that have ABM but not BMI2 - Allow new assembly task with
--continue
- Symbol link
megahit_core_no_hw_accel
tomegahit_toolkit
for backward compatibility - Better logging and for memory adjustment during SDBG building
- Attempt to continue SDBG building even user-specified memory size is not sufficient
- Refactored and fixed a bug in local assembler
- Refactored
megahit
script - Obtain total memory size from
os.sysconf
- Fixed segmentation fault in Mac OS with clang 4.0
- Added
--cleaning-rounds
and--disconnect-ratio
options for more flexible graph cleaning control
- Fixed a bug that causes higher memory usage in seq2sdbg
- Refactor on sequence sorters, edge I/O and contig I/O
- Fixed a few memory leaks
- Use std::vector to replace malloc in SDBG builders
- Try to fix potential problem caused by benign data race in unitig graph refreshing
- Faster by using phmap and xxh3
- Refactored sequence readers
- Fixed a bug in SDBG building of large k-mer sizes
- Automatically detect POPCNT/BMI2 and select the correct megahit_core binary
- Added
--no-hw-accel
option for users whose CPUs do not support BMI2/POPCNT - Added
--test
option for testing - Compilable with CMake 2.8 and g++4.8
Heavily refactored the whole project:
- Remove GPU support
- Use cmake
- Use sparsepp to replace IDBA's hash map for better performance in both speed and memory efficiency
- Use pdep instruction to speed up rand and select
- Rewrite unitig graph
- Rewrite the iterate-edge component
- Rewrite the SDBG library, except for the builder
- Fixed a bug which may involve too many reads into local assembly
The changes result in a faster and more memory-efficient tool, but have little effect on assembly quality.
- Fixed a bug in mercy edge stage in 1-pass mode
- Fix a bug in atomic bit vector that may cause a race condition
- Hotfix of an integer overflow bug
- Added
-f
option to force overwrite output directory - Added
--bubble-level
option to control bubble merging; though level 3 (i.e. super bubble) is not mature (default level is 2) - Optimized the speed of tips removal
- Added components to better handle high depth errors
- Added components to merge super bubbles
- Fine tuning k-mer sizes to support longer reads (150bp)
- In general, it produces longer contigs compared to previous versions
- Fixed a bug that ignores edge multiplicity at all. This bug existed since v1.0.4-beta
- Removed the requirement for CPU_thread >= 2.
- Added the
--tmp-dir
option - More user-friendly error message when seeing bad pair-end files
- Fixed a bug that may stop an assembly earlier
- Faster index of succinct de Bruijn graph via prefix look up
- Add
--prune-level
3 and--prune-depth
options for more aggressive pruning - Tune
bulk
parameters - Support reads with length >= 65536 bp
- Hotfix of number of tip nodes in SdBG 32 bit integer overflow
- Fixed a bug when number of large multiplicities > INT_MAX
- Fixed dead loop in local assembler when # of reads is 0
- Use
mmap
for edge/sdbg IO - Correct the rounding of edge multiplicity from float to integer
- Fixed number of SdBG edges 32-bit integer overflow.
--presets
option: preset parameters for different types of assembly- New CPU sorting design: faster kmer counting & graph construction
- New unitig graph and edge multiplicity design: more accurate assembly
- Merge bubble carefully at small k: reduce the occurrences of bubble collapsing
- Hotfix of incorrect max read length of multiple library
- Hotfix of
--input-cmd
idling
- Fixed segmentation fault when a read is all N
- Fixed continue mode: check continue mode before writing binary reads
- Slightly improve SdBG traversal functions
- fine tune local assembly multi-thread schedules
- fine tune SdBG builder memory usage
--verbose
option- fixed a bug when reading paired-end reads of different length
- print assembly stats to the end of the screen message
- print read libraries info to the screen message, and number of reads in each libraries to the log file
- added the missing file
citycrc.h
New features:
--max-read-len
parameter no longer required--memory
option set to 0.9 by default- make use of PE informations (with local assembly)
--prune-level
and--merge-level
for setting pruning and merging intensity--kmin-1pass
option for assembling ultra low-coverage datasets in less memory- supporting bzip2 input files
- useful tools in
megahit_toolkit
, including contig2fastg for conversion of contig files into SPAdes-like fastg
Bug Fixes:
- Fixed incorrect mercy kmers searching when read length >= 255
- Minor bugs (huge log in some edge cases, etc.)
New features:
- Semi-auto memory setting: when 0 < "-m" < 1, use fraction of the machine's memory
--out-prefix
option--cpu-only
turn on by default, and--use-gpu
option to enable GPU- python3 compatibility
Bug Fixes:
- Fixed "option --num-cpu-threads not recognized"
Enhancements:
--mem-flag
option for memory control--continue
option to resume an interrupted run- support mixed fasta/fastq input via
kseq.h
Bug Fixes:
- Fixed crashes related to OpenMP
Enhancements:
- MAC OS X support
- Minor improvement to reduce memory usage of the SdBG builder
Bug Fixes:
- Fixed crashes in some edge cases
Enhancements:
- Update Makefile and python wrapper to improve compatibility
- Output exit code when subprogram exit abnormally
- Improve memory usage of the subprogram
assembler
- Fix the issue of minor stat differences caused by loops
Bug Fixes:
- Use
get_ref_with_lock()
to ensurehash_map
being thread-safe when updating values - Correct the computation of edge multiplicity when iterate from small k to large k
- Fix a bug that cause segfault in the subprogram
sdbg_builder
Enhancements:
- Add change log
- More detailed README for input format
- Use
CompactSequence
inUnitigGraph
- Remove unused parallel sorting codes
Bug Fixes:
- Fixed wrong computation of
word_per_read
incx1_functions.cpp
- Fixed crash in
FastxReader
if the file is empty - Fixed floating point error in
assembly_algorithms.cpp