diff --git a/docs/api/expect.md b/docs/api/expect.md
index 1103ee6a15cb..282e65984587 100644
--- a/docs/api/expect.md
+++ b/docs/api/expect.md
@@ -876,6 +876,30 @@ test('spy function', () => {
 })
 ```
 
+## toHaveBeenCalledExactlyOnceWith <Version>2.2.0</Version> {#tohavebeencalledexactlyoncewith}
+
+- **Type**: `(...args: any[]) => Awaitable<void>`
+
+This assertion checks if a function was called exactly once and with certain parameters. Requires a spy function to be passed to `expect`.
+
+```ts
+import { expect, test, vi } from 'vitest'
+
+const market = {
+  buy(subject: string, amount: number) {
+    // ...
+  },
+}
+
+test('spy function', () => {
+  const buySpy = vi.spyOn(market, 'buy')
+
+  market.buy('apples', 10)
+
+  expect(buySpy).toHaveBeenCalledExactlyOnceWith('apples', 10)
+})
+```
+
 ## toHaveBeenLastCalledWith
 
 - **Type**: `(...args: any[]) => Awaitable<void>`
diff --git a/packages/expect/src/jest-expect.ts b/packages/expect/src/jest-expect.ts
index c8cb304ce193..dc16c62dafa1 100644
--- a/packages/expect/src/jest-expect.ts
+++ b/packages/expect/src/jest-expect.ts
@@ -595,6 +595,27 @@ export const JestChaiExpect: ChaiPlugin = (chai, utils) => {
       throw new AssertionError(formatCalls(spy, msg, args))
     }
   })
+  def('toHaveBeenCalledExactlyOnceWith', function (...args) {
+    const spy = getSpy(this)
+    const spyName = spy.getMockName()
+    const callCount = spy.mock.calls.length
+    const hasCallWithArgs = spy.mock.calls.some(callArg =>
+      jestEquals(callArg, args, [...customTesters, iterableEquality]),
+    )
+    const pass = hasCallWithArgs && callCount === 1
+    const isNot = utils.flag(this, 'negate') as boolean
+
+    const msg = utils.getMessage(this, [
+      pass,
+      `expected "${spyName}" to be called once with arguments: #{exp}`,
+      `expected "${spyName}" to not be called once with arguments: #{exp}`,
+      args,
+    ])
+
+    if ((pass && isNot) || (!pass && !isNot)) {
+      throw new AssertionError(formatCalls(spy, msg, args))
+    }
+  })
   def(
     ['toHaveBeenNthCalledWith', 'nthCalledWith'],
     function (times: number, ...args: any[]) {
diff --git a/packages/expect/src/types.ts b/packages/expect/src/types.ts
index c1f033437980..e53b059e555e 100644
--- a/packages/expect/src/types.ts
+++ b/packages/expect/src/types.ts
@@ -635,6 +635,15 @@ export interface Assertion<T = any>
    */
   toHaveBeenCalledOnce: () => void
 
+  /**
+   * Ensure that a mock function is called with specific arguments and called
+   * exactly once.
+   *
+   * @example
+   * expect(mockFunc).toHaveBeenCalledExactlyOnceWith('arg1', 42);
+   */
+  toHaveBeenCalledExactlyOnceWith: <E extends any[]>(...args: E) => void
+
   /**
    * Checks that a value satisfies a custom matcher function.
    *
diff --git a/test/core/test/jest-expect.test.ts b/test/core/test/jest-expect.test.ts
index 4d6bb3a0e8e7..79ffc4874b39 100644
--- a/test/core/test/jest-expect.test.ts
+++ b/test/core/test/jest-expect.test.ts
@@ -634,6 +634,70 @@ describe('toHaveBeenCalledWith', () => {
   })
 })
 
+describe('toHaveBeenCalledExactlyOnceWith', () => {
+  describe('negated', () => {
+    it('fails if called', () => {
+      const mock = vi.fn()
+      mock(3)
+
+      expect(() => {
+        expect(mock).not.toHaveBeenCalledExactlyOnceWith(3)
+      }).toThrow(/^expected "spy" to not be called once with arguments: \[ 3 \][^e]/)
+    })
+
+    it('passes if called multiple times with args', () => {
+      const mock = vi.fn()
+      mock(3)
+      mock(3)
+
+      expect(mock).not.toHaveBeenCalledExactlyOnceWith(3)
+    })
+
+    it('passes if not called', () => {
+      const mock = vi.fn()
+      expect(mock).not.toHaveBeenCalledExactlyOnceWith(3)
+    })
+
+    it('passes if called with a different argument', () => {
+      const mock = vi.fn()
+      mock(4)
+
+      expect(mock).not.toHaveBeenCalledExactlyOnceWith(3)
+    })
+  })
+
+  it('fails if not called or called too many times', () => {
+    const mock = vi.fn()
+
+    expect(() => {
+      expect(mock).toHaveBeenCalledExactlyOnceWith(3)
+    }).toThrow(/^expected "spy" to be called once with arguments: \[ 3 \][^e]/)
+
+    mock(3)
+    mock(3)
+
+    expect(() => {
+      expect(mock).toHaveBeenCalledExactlyOnceWith(3)
+    }).toThrow(/^expected "spy" to be called once with arguments: \[ 3 \][^e]/)
+  })
+
+  it('fails if called with wrong args', () => {
+    const mock = vi.fn()
+    mock(4)
+
+    expect(() => {
+      expect(mock).toHaveBeenCalledExactlyOnceWith(3)
+    }).toThrow(/^expected "spy" to be called once with arguments: \[ 3 \][^e]/)
+  })
+
+  it('passes if called exactly once with args', () => {
+    const mock = vi.fn()
+    mock(3)
+
+    expect(mock).toHaveBeenCalledExactlyOnceWith(3)
+  })
+})
+
 describe('async expect', () => {
   it('resolves', async () => {
     await expect((async () => 'true')()).resolves.toBe('true')