diff --git a/packages/vitest/src/utils/test-helpers.ts b/packages/vitest/src/utils/test-helpers.ts
index b7aacae339c4..1b37c5155887 100644
--- a/packages/vitest/src/utils/test-helpers.ts
+++ b/packages/vitest/src/utils/test-helpers.ts
@@ -57,10 +57,13 @@ export async function groupFilesByEnv(
         file,
       )
 
-      const envOptions = JSON.parse(
-        code.match(/@(?:vitest|jest)-environment-options\s+?(.+)/)?.[1]
-        || 'null',
-      )
+      let envOptionsJson = code.match(/@(?:vitest|jest)-environment-options\s+(.+)/)?.[1]
+      if (envOptionsJson?.endsWith('*/')) {
+        // Trim closing Docblock characters the above regex might have captured
+        envOptionsJson = envOptionsJson.slice(0, -2)
+      }
+
+      const envOptions = JSON.parse(envOptionsJson || 'null')
       const envKey = env === 'happy-dom' ? 'happyDOM' : env
       const environment: ContextTestEnvironment = {
         name: env as VitestEnvironment,
diff --git a/test/core/test/dom-single-line-options.test.ts b/test/core/test/dom-single-line-options.test.ts
new file mode 100644
index 000000000000..d3338fd39026
--- /dev/null
+++ b/test/core/test/dom-single-line-options.test.ts
@@ -0,0 +1,7 @@
+/** @vitest-environment jsdom */
+
+/** @vitest-environment-options { "url": "https://example.com/" } */
+
+import { expect, it } from 'vitest'
+
+it('parse single line environment options', () => expect(location.href).toBe('https://example.com/'))