diff --git a/packages/vite/src/node/fsUtils.ts b/packages/vite/src/node/fsUtils.ts
index ae1892e989677c..9536218c8f8ce4 100644
--- a/packages/vite/src/node/fsUtils.ts
+++ b/packages/vite/src/node/fsUtils.ts
@@ -189,6 +189,10 @@ export function createCachedFsUtils(config: ResolvedConfig): FsUtils {
   function getDirentCacheFromPath(
     normalizedFile: string,
   ): DirentCache | false | undefined {
+    // path.posix.normalize may return a path either with / or without /
+    if (normalizedFile[normalizedFile.length - 1] === '/') {
+      normalizedFile = normalizedFile.slice(0, -1)
+    }
     if (normalizedFile === root) {
       return rootCache
     }
diff --git a/playground/resolve/__tests__/resolve.spec.ts b/playground/resolve/__tests__/resolve.spec.ts
index 45b87c23e4ab10..d930ac1e49a2df 100644
--- a/playground/resolve/__tests__/resolve.spec.ts
+++ b/playground/resolve/__tests__/resolve.spec.ts
@@ -215,6 +215,10 @@ test('Resolve doesnt interrupt page request with trailing query and .css', async
   expect(await page.textContent('h1')).toBe('Resolve')
 })
 
+test('resolve non-normalized absolute path', async () => {
+  expect(await page.textContent('.non-normalized')).toMatch('[success]')
+})
+
 test.runIf(!isWindows)(
   'Resolve doesnt interrupt page request that clashes with local project package.json',
   async () => {
diff --git a/playground/resolve/index.html b/playground/resolve/index.html
index 89eced2e7149e9..fa6b5afbb84cd6 100644
--- a/playground/resolve/index.html
+++ b/playground/resolve/index.html
@@ -167,6 +167,9 @@ <h2>resolve.conditions</h2>
 <h2>resolve package that contains # in path</h2>
 <p class="path-contains-sharp-symbol"></p>
 
+<h2>resolve non normalized absolute path</h2>
+<p class="non-normalized"></p>
+
 <script type="module">
   import '@generated-content-virtual-file'
   function text(selector, text) {
@@ -384,6 +387,9 @@ <h2>resolve package that contains # in path</h2>
     '.path-contains-sharp-symbol',
     `[success] ${contains.call('#', '#')} ${last.call('#')}`,
   )
+
+  import nonNormalizedAbsolute from '@non-normalized'
+  text('.non-normalized', nonNormalizedAbsolute)
 </script>
 
 <style>
diff --git a/playground/resolve/non-normalized.js b/playground/resolve/non-normalized.js
new file mode 100644
index 00000000000000..eee257d6c8e33c
--- /dev/null
+++ b/playground/resolve/non-normalized.js
@@ -0,0 +1 @@
+export default '[success] non normalized absolute path'
diff --git a/playground/resolve/vite.config.js b/playground/resolve/vite.config.js
index c016401a85ffb2..a840ec103298d1 100644
--- a/playground/resolve/vite.config.js
+++ b/playground/resolve/vite.config.js
@@ -99,6 +99,13 @@ export default defineConfig({
         }
       },
     },
+    {
+      name: 'resolve to non normalized absolute',
+      async resolveId(id) {
+        if (id !== '@non-normalized') return
+        return this.resolve(__dirname + '//non-normalized')
+      },
+    },
   ],
   optimizeDeps: {
     include: [