diff --git a/tests/typecheck/managers/querysets/test_from_queryset.yml b/tests/typecheck/managers/querysets/test_from_queryset.yml
index 59f0cd63c..ec08da3da 100644
--- a/tests/typecheck/managers/querysets/test_from_queryset.yml
+++ b/tests/typecheck/managers/querysets/test_from_queryset.yml
@@ -675,3 +675,28 @@
                   ...
 
               StrCallable = BaseManager.from_queryset(ModelQuerySet, class_name=str(1))
+
+-   case: test_queryset_arg_as_unsupported_expressions
+    main: |
+        from typing import Union, Generic, TypeVar
+        from typing_extensions import TypeAlias
+        from django.db import models
+        from django.db.models.manager import Manager
+
+        class QS1(models.QuerySet[models.Model]):
+            ...
+        class QS2(models.QuerySet[models.Model]):
+            ...
+
+        Alias: TypeAlias = Union[QS1, QS2]
+        reveal_type(Manager[models.Model].from_queryset(Alias))
+
+        T = TypeVar("T")
+        class NonQSGeneric(Generic[T]):
+            ...
+        reveal_type(Manager[models.Model].from_queryset(NonQSGeneric[int]))
+    out: |
+        main:12: note: Revealed type is "Type[django.db.models.manager.Manager[django.db.models.base.Model]]"
+        main:12: error: Argument 1 to "from_queryset" of "BaseManager" has incompatible type "<typing special form>"; expected "Type[_QuerySet[Model, Model]]"  [arg-type]
+        main:17: note: Revealed type is "Type[django.db.models.manager.Manager[django.db.models.base.Model]]"
+        main:17: error: Argument 1 to "from_queryset" of "BaseManager" has incompatible type "Type[NonQSGeneric[Any]]"; expected "Type[_QuerySet[Model, Model]]"  [arg-type]