Run evaluation methods always include a reference?

[KunHHE]

Hi @LouisK92, Can I ask you wen performing evaluation for selected genes, This is a very must step to include a reference? # select reference probesets with basic selection method, selections = sp.se.select_reference_probesets(adata, n=20)? For example, we selected 140 gene, should we use selections = sp.se.select_reference_probesets(adata, n=140) then?

If no reference included for evaluation, then the output like evaluator.plot_summary(), evaluator.plot_confusion_matrix(), evaluator.plot_coexpression() are only show "spapros", no DE, PCA and HVG as a comparison?

Thanks!!!