diff --git a/code/e2e-tests/preview-web.spec.ts b/code/e2e-tests/preview-web.spec.ts
new file mode 100644
index 000000000000..3dec28a68910
--- /dev/null
+++ b/code/e2e-tests/preview-web.spec.ts
@@ -0,0 +1,32 @@
+import { test, expect } from '@playwright/test';
+import process from 'process';
+import { SbPage } from './util';
+
+const storybookUrl = process.env.STORYBOOK_URL || 'http://localhost:8001';
+
+test.describe('preview-web', () => {
+  test.beforeEach(async ({ page }) => {
+    await page.goto(storybookUrl);
+    await new SbPage(page).waitUntilLoaded();
+  });
+
+  test('should pass over shortcuts, but not from play functions, story', async ({ page }) => {
+    const sbPage = new SbPage(page);
+    await sbPage.navigateToStory('lib/store/shortcuts', 'keydown-during-play');
+
+    await expect(sbPage.page.locator('.sidebar-container')).toBeVisible();
+
+    await sbPage.previewRoot().locator('button').press('s');
+    await expect(sbPage.page.locator('.sidebar-container')).not.toBeVisible();
+  });
+
+  test('should pass over shortcuts, but not from play functions, docs', async ({ page }) => {
+    const sbPage = new SbPage(page);
+    await sbPage.navigateToStory('lib/store/shortcuts', 'docs');
+
+    await expect(sbPage.page.locator('.sidebar-container')).toBeVisible();
+
+    await sbPage.previewRoot().getByRole('button').getByText('Submit').press('s');
+    await expect(sbPage.page.locator('.sidebar-container')).not.toBeVisible();
+  });
+});
diff --git a/code/lib/preview-web/src/PreviewWeb.test.ts b/code/lib/preview-web/src/PreviewWeb.test.ts
index 356d22018c37..36cf0b17ce72 100644
--- a/code/lib/preview-web/src/PreviewWeb.test.ts
+++ b/code/lib/preview-web/src/PreviewWeb.test.ts
@@ -3302,6 +3302,51 @@ describe('PreviewWeb', () => {
         expect.objectContaining({})
       );
     });
+
+    it('does not emit PREVIEW_KEYDOWN during story play functions', async () => {
+      document.location.search = '?id=component-one--a';
+
+      const [gate, openGate] = createGate();
+      componentOneExports.a.play.mockImplementationOnce(async () => gate);
+      const preview = new PreviewWeb();
+      await preview.initialize({ importFn, getProjectAnnotations });
+      await waitForRenderPhase('playing');
+
+      await preview.onKeydown({
+        target: { tagName: 'div', getAttribute: jest.fn().mockReturnValue(null) },
+      } as any);
+
+      expect(mockChannel.emit).not.toHaveBeenCalledWith(
+        PREVIEW_KEYDOWN,
+        expect.objectContaining({})
+      );
+      openGate();
+    });
+
+    it('does not emit PREVIEW_KEYDOWN during docs play functions', async () => {
+      document.location.search = '?id=component-one--a';
+
+      const preview = await createAndRenderPreview();
+
+      mockChannel.emit.mockClear();
+      const [gate, openGate] = createGate();
+      componentOneExports.b.play.mockImplementationOnce(async () => gate);
+      preview.renderStoryToElement(
+        await preview.storyStore.loadStory({ storyId: 'component-one--b' }),
+        {} as any
+      );
+      await waitForRenderPhase('playing');
+
+      await preview.onKeydown({
+        target: { tagName: 'div', getAttribute: jest.fn().mockReturnValue(null) },
+      } as any);
+
+      expect(mockChannel.emit).not.toHaveBeenCalledWith(
+        PREVIEW_KEYDOWN,
+        expect.objectContaining({})
+      );
+      openGate();
+    });
   });
 
   describe('extract', () => {
diff --git a/code/lib/preview-web/src/PreviewWeb.tsx b/code/lib/preview-web/src/PreviewWeb.tsx
index f8d5e15e5733..eba66fadac81 100644
--- a/code/lib/preview-web/src/PreviewWeb.tsx
+++ b/code/lib/preview-web/src/PreviewWeb.tsx
@@ -199,7 +199,7 @@ export class PreviewWeb<TFramework extends AnyFramework> extends Preview<TFramew
   }
 
   onKeydown(event: KeyboardEvent) {
-    if (!this.currentRender?.disableKeyListeners && !focusInInput(event)) {
+    if (!this.storyRenders.find((r) => r.disableKeyListeners) && !focusInInput(event)) {
       // We have to pick off the keys of the event that we need on the other side
       const { altKey, ctrlKey, metaKey, shiftKey, key, code, keyCode } = event;
       this.channel.emit(PREVIEW_KEYDOWN, {
diff --git a/code/lib/store/template/stories/shortcuts.stories.ts b/code/lib/store/template/stories/shortcuts.stories.ts
new file mode 100644
index 000000000000..6a0ffec340bc
--- /dev/null
+++ b/code/lib/store/template/stories/shortcuts.stories.ts
@@ -0,0 +1,23 @@
+import globalThis from 'global';
+import { userEvent, within } from '@storybook/testing-library';
+import { PREVIEW_KEYDOWN } from '@storybook/core-events';
+import { jest, expect } from '@storybook/jest';
+import type { PlayFunctionContext } from '@storybook/csf';
+
+export default {
+  component: globalThis.Components.Form,
+  tags: ['docsPage'],
+};
+
+export const KeydownDuringPlay = {
+  play: async ({ canvasElement }: PlayFunctionContext) => {
+    const channel = globalThis.__STORYBOOK_ADDONS_CHANNEL__;
+
+    const previewKeydown = jest.fn();
+    channel.on(PREVIEW_KEYDOWN, previewKeydown);
+    const button = await within(canvasElement).findByText('Submit');
+    await userEvent.type(button, 's');
+
+    expect(previewKeydown).not.toBeCalled();
+  },
+};