Pick primers without 'CG' in them

[haseebz]

I am using Primer_TK to design primers that can be used for methylation detection. To achieve this I would like primers that do not have consecutive 'CG' nucleotides in them. Is there a way to do this?

[haseebz]

Adding a suggestion on this.
Since primer_tk uses primer3 parameters to pick primers, is it possible to use EXCLUDED_REGIONS from the primer3 options to exclude CGs from primer design?

[haseebz]

I have figured out one way we can do this. We can use SEQUENCE_EXCLUDED_REGION= in the primer3_input file. But doing this manually for a few sequences is easy. How can we do this for a larger input file?