diff --git a/docs/tutorials/seurat-go/seurat-go.md b/docs/tutorials/seurat-go/seurat-go.md index 50eec127..82aec39f 100644 --- a/docs/tutorials/seurat-go/seurat-go.md +++ b/docs/tutorials/seurat-go/seurat-go.md @@ -22,16 +22,27 @@ * [x] Intermediate * [ ] Advanced -**Data:** Placeholder. +**Data:** 10k Human PBMCs, Multiome v1.0, Chromium X. -**Tools:** Placeholder. +**Tools:** R >= 4.4.0 and associated packages: + +- Seurat +- SeuratData +- clusterProfiler +- Signac +- ggplot2 **Pipeline:** *Section 1:* Setup, Introduction, and Preprocessing. *Section 2:* Gene Ontology analysis. *Section 3:* Integration of ATAC-seq data. -**Learning objectives:** Placeholder. +**Learning objectives:** + +- Apply GO enrichment analysis to identify biological processes, molecular functions, and cellular components represented in single-cell RNA-seq data. +- Interpret GO results and identify key pathways and functional modules relevant to specific biological questions. +- Design a workflow for integrating various omics layers, considering the biological context and research goals. +- Take advantage of the Seurat, Signac and clusterProfiler R packages to perform the analysis, generate plots and interpret the results.