apptainer exec Demuxafy.sif Assign_Indiv_by_Geno.R no output

[ElenaSLuis]

Hey! I'm trying to use Assign_Indiv_by_Geno.R function of Demuxafy.sif from data of demuxlet code. It seems the problem is with the FORMAT, but I don't undestand very well...

apptainer exec Demuxafy.sif Assign_Indiv_by_Geno.R -r /home/elenasl/scRNAseq/freemuxlet/vcf_chr/GRCh38_1000G_MAF0.01_GeneFiltered_ChrEncoding_Sorted_polyIC.vcf \

-c /home/elenasl/scRNAseq/freemuxlet/output_polyIC.clust1.vcf.gz -o /home/elenasl/scRNAseq/freemuxlet/
System has not been booted with systemd as init system (PID 1). Can't operate.
Failed to connect to bus: Host is down
Scanning file to determine attributes.
File attributes:
meta lines: 242
header_line: 243
variant count: 7139809
column count: 8
Meta line 242 read in.
All meta lines processed.
gt matrix initialized.
Character matrix gt created.
Character matrix gt rows: 7139809
Character matrix gt cols: 8
skip: 0
nrows: 7139809
row_num: 0
Processed variant: 7139809
All variants processed
Scanning file to determine attributes.
File attributes:
meta lines: 32
header_line: 33
variant count: 2204998
column count: 11
Meta line 32 read in.
All meta lines processed.
gt matrix initialized.
Character matrix gt created.
Character matrix gt rows: 2204998
Character matrix gt cols: 11
skip: 0
nrows: 2204998
row_num: 0
Processed variant: 2204998
All variants processed
Found GT genotype format in cluster vcf. Will use that metric for cluster correlation.
Detected / separator for GT genotype format in cluster vcf

Error in if (colnames(x@gt)[1] != "FORMAT") { :
argument is of length zero
Calls: as_tibble -> extract.gt
Execution halted

thank you in advance,

Elena